Tags

Type your tag names separated by a space and hit enter

Molecular survey and genetic identification of Anaplasma species in goats from central and southern China.
Appl Environ Microbiol. 2012 Jan; 78(2):464-70.AE

Abstract

Anaplasma species are obligate intracellular rickettsial pathogens that impact the health of humans and animals. Few studies have been carried out on Anaplasma infections in central and southern China. This study was conducted to determine the coinfection rates of Anaplasma ovis, A. bovis, and A. phagocytophilum from 262 field blood samples of goats in these regions. The average prevalences of single infection of A. ovis, A. bovis, and A. phagocytophilum were 15.3, 16.0, and 6.1%, respectively. Coinfection of A. ovis and A. bovis was dominant, with an infection rate of 27.1%. Coinfection of A. ovis and A. phagocytophilum was 1.9% and that of A. bovis and A. phagocytophilum was 4.2%. Three-pathogen coinfection was found in three of four investigated provinces with a prevalence between 0 and 5.3%. The accuracy of the PCR results was corroborated by sequencing. Analysis of the 16S rRNA gene sequences of A. bovis and A. phagocytophilum confirmed the presence of these pathogens at the investigated sites and indicated the possible genetic diversity of A. phagocytophilum. Field blood inoculation of experimental animals led to successful identification and observation of the morphological shapes of A. bovis in the infected monocytes of sheep. Phylogenetic study with msp4 sequences of A. ovis indicated that the A. ovis genotypes from sheep in the north differed from the genotypes of goats in the investigated sites.

Authors+Show Affiliations

State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22057867

Citation

Liu, Zhijie, et al. "Molecular Survey and Genetic Identification of Anaplasma Species in Goats From Central and Southern China." Applied and Environmental Microbiology, vol. 78, no. 2, 2012, pp. 464-70.
Liu Z, Ma M, Wang Z, et al. Molecular survey and genetic identification of Anaplasma species in goats from central and southern China. Appl Environ Microbiol. 2012;78(2):464-70.
Liu, Z., Ma, M., Wang, Z., Wang, J., Peng, Y., Li, Y., Guan, G., Luo, J., & Yin, H. (2012). Molecular survey and genetic identification of Anaplasma species in goats from central and southern China. Applied and Environmental Microbiology, 78(2), 464-70. https://doi.org/10.1128/AEM.06848-11
Liu Z, et al. Molecular Survey and Genetic Identification of Anaplasma Species in Goats From Central and Southern China. Appl Environ Microbiol. 2012;78(2):464-70. PubMed PMID: 22057867.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular survey and genetic identification of Anaplasma species in goats from central and southern China. AU - Liu,Zhijie, AU - Ma,Miling, AU - Wang,Zhaowen, AU - Wang,Jing, AU - Peng,Yulv, AU - Li,Youquan, AU - Guan,Guiquan, AU - Luo,Jianxun, AU - Yin,Hong, Y1 - 2011/11/04/ PY - 2011/11/8/entrez PY - 2011/11/8/pubmed PY - 2012/4/20/medline SP - 464 EP - 70 JF - Applied and environmental microbiology JO - Appl. Environ. Microbiol. VL - 78 IS - 2 N2 - Anaplasma species are obligate intracellular rickettsial pathogens that impact the health of humans and animals. Few studies have been carried out on Anaplasma infections in central and southern China. This study was conducted to determine the coinfection rates of Anaplasma ovis, A. bovis, and A. phagocytophilum from 262 field blood samples of goats in these regions. The average prevalences of single infection of A. ovis, A. bovis, and A. phagocytophilum were 15.3, 16.0, and 6.1%, respectively. Coinfection of A. ovis and A. bovis was dominant, with an infection rate of 27.1%. Coinfection of A. ovis and A. phagocytophilum was 1.9% and that of A. bovis and A. phagocytophilum was 4.2%. Three-pathogen coinfection was found in three of four investigated provinces with a prevalence between 0 and 5.3%. The accuracy of the PCR results was corroborated by sequencing. Analysis of the 16S rRNA gene sequences of A. bovis and A. phagocytophilum confirmed the presence of these pathogens at the investigated sites and indicated the possible genetic diversity of A. phagocytophilum. Field blood inoculation of experimental animals led to successful identification and observation of the morphological shapes of A. bovis in the infected monocytes of sheep. Phylogenetic study with msp4 sequences of A. ovis indicated that the A. ovis genotypes from sheep in the north differed from the genotypes of goats in the investigated sites. SN - 1098-5336 UR - https://www.unboundmedicine.com/medline/citation/22057867/Molecular_survey_and_genetic_identification_of_Anaplasma_species_in_goats_from_central_and_southern_China_ L2 - http://aem.asm.org/cgi/pmidlookup?view=long&pmid=22057867 DB - PRIME DP - Unbound Medicine ER -