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Molecularly imprinted solid-phase extraction for the selective HPLC determination of ractopamine in pig urine.
J Sep Sci. 2011 Dec; 34(23):3399-409.JS

Abstract

A method was developed for the determination of ractopamine in pig urine using molecularly imprinted solid-phase extraction (MISPE) as the sample clean-up technique combined with high-performance liquid chromatography. The molecularly imprinted polymer (MIP) was synthesized in acetonitrile-triethylamine system using ractopamine (RAC) as the template and acrylamide as the monomer. The binding capacity of the polymer toward RAC was found to be about 2.57 mg of ractopamine/g of polymer. The optimal procedures for MISPE consisted of conditioning with 3 mL methanol, equilibrating with 3 mL of water, loading volume of <10 mL of aqueous sample (pH 7), washing with 3 mL water and 3 mL methanol, and eluting with 5 mL of 5% ammonia in methanol. In the four spiked samples with the levels of 0.01, 0.1, 1.0 and 5.0 μg/mL, the mean recoveries of analyte on the MIP were higher than 90% with relative standard deviation <10%, and significant differences between imprinted and non-imprinted materials were observed. The MIP selectivity was evaluated by checking 11 drugs with similar and different molecular structures to that of RAC. The characteristics of three-dimensional cavities and hydrogen bond interaction were regarded as the main factors that dominated the retention of RAC on the MISPE cartridge.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Zhang Q
Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong, PC, P. R. China.
Su Y
No affiliation info available
He Q
No affiliation info available
Shen X
No affiliation info available
He L
No affiliation info available
Zhang N
No affiliation info available
Zeng Z
No affiliation info available

MeSH

Adrenergic beta-AgonistsAdsorptionAnimalsChromatography, High Pressure LiquidMolecular ImprintingPhenethylaminesPolymersSolid Phase ExtractionSwine

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22058121

Citation

Zhang, Qingjie, et al. "Molecularly Imprinted Solid-phase Extraction for the Selective HPLC Determination of Ractopamine in Pig Urine." Journal of Separation Science, vol. 34, no. 23, 2011, pp. 3399-409.
Zhang Q, Su Y, He Q, et al. Molecularly imprinted solid-phase extraction for the selective HPLC determination of ractopamine in pig urine. J Sep Sci. 2011;34(23):3399-409.
Zhang, Q., Su, Y., He, Q., Shen, X., He, L., Zhang, N., & Zeng, Z. (2011). Molecularly imprinted solid-phase extraction for the selective HPLC determination of ractopamine in pig urine. Journal of Separation Science, 34(23), 3399-409. https://doi.org/10.1002/jssc.201100655
Zhang Q, et al. Molecularly Imprinted Solid-phase Extraction for the Selective HPLC Determination of Ractopamine in Pig Urine. J Sep Sci. 2011;34(23):3399-409. PubMed PMID: 22058121.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecularly imprinted solid-phase extraction for the selective HPLC determination of ractopamine in pig urine. AU - Zhang,Qingjie, AU - Su,Yijuan, AU - He,Qianqian, AU - Shen,Xianguang, AU - He,Limin, AU - Zhang,Nan, AU - Zeng,Zhenling, Y1 - 2011/11/07/ PY - 2011/07/27/received PY - 2011/09/08/revised PY - 2011/09/09/accepted PY - 2011/11/8/entrez PY - 2011/11/8/pubmed PY - 2012/3/3/medline SP - 3399 EP - 409 JF - Journal of separation science JO - J Sep Sci VL - 34 IS - 23 N2 - A method was developed for the determination of ractopamine in pig urine using molecularly imprinted solid-phase extraction (MISPE) as the sample clean-up technique combined with high-performance liquid chromatography. The molecularly imprinted polymer (MIP) was synthesized in acetonitrile-triethylamine system using ractopamine (RAC) as the template and acrylamide as the monomer. The binding capacity of the polymer toward RAC was found to be about 2.57 mg of ractopamine/g of polymer. The optimal procedures for MISPE consisted of conditioning with 3 mL methanol, equilibrating with 3 mL of water, loading volume of <10 mL of aqueous sample (pH 7), washing with 3 mL water and 3 mL methanol, and eluting with 5 mL of 5% ammonia in methanol. In the four spiked samples with the levels of 0.01, 0.1, 1.0 and 5.0 μg/mL, the mean recoveries of analyte on the MIP were higher than 90% with relative standard deviation <10%, and significant differences between imprinted and non-imprinted materials were observed. The MIP selectivity was evaluated by checking 11 drugs with similar and different molecular structures to that of RAC. The characteristics of three-dimensional cavities and hydrogen bond interaction were regarded as the main factors that dominated the retention of RAC on the MISPE cartridge. SN - 1615-9314 UR - https://www.unboundmedicine.com/medline/citation/22058121/Molecularly_imprinted_solid_phase_extraction_for_the_selective_HPLC_determination_of_ractopamine_in_pig_urine_ L2 - https://doi.org/10.1002/jssc.201100655 DB - PRIME DP - Unbound Medicine ER -