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Confocal laser scanning microscopy, optical coherence tomography and transonychial water loss for in vivo investigation of nails.
Br J Dermatol. 2012 Apr; 166(4):740-6.BJ

Abstract

BACKGROUND

Nail disorders can be diagnostically challenging to the dermatologist. Noninvasive methods might help to avoid nail biopsies. More knowledge of the typical features of healthy nails with these techniques is needed for comparison with nail diseases.

OBJECTIVES

To describe the typical morphology of healthy nails in optical coherence tomography (OCT) and confocal laser scanning microscopy (CLSM) and to examine the influence of exposure to water on OCT features, nail thickness as well as on transonychial water loss (TOWL) before and after a hand bath. Material and methods In the first part healthy nail plates were investigated with OCT and CLSM. The thickness of layers as well as structural details were defined. Secondly, in a prospective study 30 healthy volunteers conducted 10 hand baths with water within a 2-week period. Measurements of nail thickness and signal intensity by OCT as well as of the TOWL were performed over time.

RESULTS

In OCT the unaffected nail plate appears as a band-like, layered structure, yet with some individual differences. In addition, CLSM is able to display single corneocytes and the integrity of their borders. Exposure to water led to a transient rise of TOWL and in nail thickness.

CONCLUSIONS

OCT, CLSM and TOWL offer valuable noninvasive diagnostic tools for the examination of nails. Using CLSM, microscopic features like integrity of single corneocytes can be investigated. OCT allows determination of nail plate thickness and both OCT and TOWL measurements are able to detect short-term effects after exposure to water.

Authors+Show Affiliations

Department of Dermatology and Allergology, Ludwig-Maximilians-University of Munich, Germany.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22059869

Citation

Sattler, E, et al. "Confocal Laser Scanning Microscopy, Optical Coherence Tomography and Transonychial Water Loss for in Vivo Investigation of Nails." The British Journal of Dermatology, vol. 166, no. 4, 2012, pp. 740-6.
Sattler E, Kaestle R, Rothmund G, et al. Confocal laser scanning microscopy, optical coherence tomography and transonychial water loss for in vivo investigation of nails. Br J Dermatol. 2012;166(4):740-6.
Sattler, E., Kaestle, R., Rothmund, G., & Welzel, J. (2012). Confocal laser scanning microscopy, optical coherence tomography and transonychial water loss for in vivo investigation of nails. The British Journal of Dermatology, 166(4), 740-6. https://doi.org/10.1111/j.1365-2133.2011.10730.x
Sattler E, et al. Confocal Laser Scanning Microscopy, Optical Coherence Tomography and Transonychial Water Loss for in Vivo Investigation of Nails. Br J Dermatol. 2012;166(4):740-6. PubMed PMID: 22059869.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Confocal laser scanning microscopy, optical coherence tomography and transonychial water loss for in vivo investigation of nails. AU - Sattler,E, AU - Kaestle,R, AU - Rothmund,G, AU - Welzel,J, Y1 - 2012/03/14/ PY - 2011/11/9/entrez PY - 2011/11/9/pubmed PY - 2012/9/21/medline SP - 740 EP - 6 JF - The British journal of dermatology JO - Br. J. Dermatol. VL - 166 IS - 4 N2 - BACKGROUND: Nail disorders can be diagnostically challenging to the dermatologist. Noninvasive methods might help to avoid nail biopsies. More knowledge of the typical features of healthy nails with these techniques is needed for comparison with nail diseases. OBJECTIVES: To describe the typical morphology of healthy nails in optical coherence tomography (OCT) and confocal laser scanning microscopy (CLSM) and to examine the influence of exposure to water on OCT features, nail thickness as well as on transonychial water loss (TOWL) before and after a hand bath. Material and methods In the first part healthy nail plates were investigated with OCT and CLSM. The thickness of layers as well as structural details were defined. Secondly, in a prospective study 30 healthy volunteers conducted 10 hand baths with water within a 2-week period. Measurements of nail thickness and signal intensity by OCT as well as of the TOWL were performed over time. RESULTS: In OCT the unaffected nail plate appears as a band-like, layered structure, yet with some individual differences. In addition, CLSM is able to display single corneocytes and the integrity of their borders. Exposure to water led to a transient rise of TOWL and in nail thickness. CONCLUSIONS: OCT, CLSM and TOWL offer valuable noninvasive diagnostic tools for the examination of nails. Using CLSM, microscopic features like integrity of single corneocytes can be investigated. OCT allows determination of nail plate thickness and both OCT and TOWL measurements are able to detect short-term effects after exposure to water. SN - 1365-2133 UR - https://www.unboundmedicine.com/medline/citation/22059869/Confocal_laser_scanning_microscopy_optical_coherence_tomography_and_transonychial_water_loss_for_in_vivo_investigation_of_nails_ L2 - https://doi.org/10.1111/j.1365-2133.2011.10730.x DB - PRIME DP - Unbound Medicine ER -