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A fast method using a new hydrophilic-lipophilic balanced sorbent in combination with ultra-high performance liquid chromatography for quantification of significant bioactive metabolites in wines.
Talanta. 2011 Oct 30; 86:82-90.T

Abstract

This manuscript describes the development and validation of an ultra-fast, efficient, and high throughput analytical method based on ultra-high performance liquid chromatography (UHPLC) equipped with a photodiode array (PDA) detection system, for the simultaneous analysis of fifteen bioactive metabolites: gallic acid, protocatechuic acid, (-)-catechin, gentisic acid, (-)-epicatechin, syringic acid, p-coumaric acid, ferulic acid, m-coumaric acid, rutin, trans-resveratrol, myricetin, quercetin, cinnamic acid and kaempferol, in wines. A 50-mm column packed with 1.7-μm particles operating at elevated pressure (UHPLC strategy) was selected to attain ultra-fast analysis and highly efficient separations. In order to reduce the complexity of wine extract and improve the recovery efficiency, a reverse-phase solid-phase extraction (SPE) procedure using as sorbent a new macroporous copolymer made from a balanced ratio of two monomers, the lipophilic divinylbenzene and the hydrophilic N-vinylpyrrolidone (Oasis™ HLB), was performed prior to UHPLC-PDA analysis. The calibration curves of bioactive metabolites showed good linearity within the established range. Limits of detection (LOD) and quantification (LOQ) ranged from 0.006 μg mL(-1) to 0.58 μg mL(-1), and from 0.019 μg mL(-1) to 1.94 μg mL(-1), for gallic and gentisic acids, respectively. The average recoveries ± SD for the three levels of concentration tested (n=9) in red and white wines were, respectively, 89 ± 3% and 90 ± 2%. The repeatability expressed as relative standard deviation (RSD) was below 10% for all the metabolites assayed. The validated method was then applied to red and white wines from different geographical origins (Azores, Canary and Madeira Islands). The most abundant component in the analysed red wines was (-)-epicatechin followed by (-)-catechin and rutin, whereas in white wines syringic and p-coumaric acids were found the major phenolic metabolites. The method was completely validated, providing a sensitive analysis for bioactive phenolic metabolites detection and showing satisfactory data for all the parameters tested. Moreover, was revealed as an ultra-fast approach allowing the separation of the fifteen bioactive metabolites investigated with high resolution power within 5 min.

Authors+Show Affiliations

CQM - Centro de Química da Madeira, Centro de Ciências Exactas e da Engenharia da Universidade da Madeira, Campus Universitário da Penteada, 9000-390 Funchal, Portugal.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22063514

Citation

Silva, Catarina L., et al. "A Fast Method Using a New Hydrophilic-lipophilic Balanced Sorbent in Combination With Ultra-high Performance Liquid Chromatography for Quantification of Significant Bioactive Metabolites in Wines." Talanta, vol. 86, 2011, pp. 82-90.
Silva CL, Pereira J, Wouter VG, et al. A fast method using a new hydrophilic-lipophilic balanced sorbent in combination with ultra-high performance liquid chromatography for quantification of significant bioactive metabolites in wines. Talanta. 2011;86:82-90.
Silva, C. L., Pereira, J., Wouter, V. G., Giró, C., & Câmara, J. S. (2011). A fast method using a new hydrophilic-lipophilic balanced sorbent in combination with ultra-high performance liquid chromatography for quantification of significant bioactive metabolites in wines. Talanta, 86, 82-90. https://doi.org/10.1016/j.talanta.2011.08.007
Silva CL, et al. A Fast Method Using a New Hydrophilic-lipophilic Balanced Sorbent in Combination With Ultra-high Performance Liquid Chromatography for Quantification of Significant Bioactive Metabolites in Wines. Talanta. 2011 Oct 30;86:82-90. PubMed PMID: 22063514.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A fast method using a new hydrophilic-lipophilic balanced sorbent in combination with ultra-high performance liquid chromatography for quantification of significant bioactive metabolites in wines. AU - Silva,Catarina L, AU - Pereira,Jorge, AU - Wouter,Van G, AU - Giró,Carme, AU - Câmara,José S, Y1 - 2011/09/01/ PY - 2011/06/08/received PY - 2011/08/02/revised PY - 2011/08/06/accepted PY - 2011/11/9/entrez PY - 2011/11/9/pubmed PY - 2012/8/17/medline SP - 82 EP - 90 JF - Talanta JO - Talanta VL - 86 N2 - This manuscript describes the development and validation of an ultra-fast, efficient, and high throughput analytical method based on ultra-high performance liquid chromatography (UHPLC) equipped with a photodiode array (PDA) detection system, for the simultaneous analysis of fifteen bioactive metabolites: gallic acid, protocatechuic acid, (-)-catechin, gentisic acid, (-)-epicatechin, syringic acid, p-coumaric acid, ferulic acid, m-coumaric acid, rutin, trans-resveratrol, myricetin, quercetin, cinnamic acid and kaempferol, in wines. A 50-mm column packed with 1.7-μm particles operating at elevated pressure (UHPLC strategy) was selected to attain ultra-fast analysis and highly efficient separations. In order to reduce the complexity of wine extract and improve the recovery efficiency, a reverse-phase solid-phase extraction (SPE) procedure using as sorbent a new macroporous copolymer made from a balanced ratio of two monomers, the lipophilic divinylbenzene and the hydrophilic N-vinylpyrrolidone (Oasis™ HLB), was performed prior to UHPLC-PDA analysis. The calibration curves of bioactive metabolites showed good linearity within the established range. Limits of detection (LOD) and quantification (LOQ) ranged from 0.006 μg mL(-1) to 0.58 μg mL(-1), and from 0.019 μg mL(-1) to 1.94 μg mL(-1), for gallic and gentisic acids, respectively. The average recoveries ± SD for the three levels of concentration tested (n=9) in red and white wines were, respectively, 89 ± 3% and 90 ± 2%. The repeatability expressed as relative standard deviation (RSD) was below 10% for all the metabolites assayed. The validated method was then applied to red and white wines from different geographical origins (Azores, Canary and Madeira Islands). The most abundant component in the analysed red wines was (-)-epicatechin followed by (-)-catechin and rutin, whereas in white wines syringic and p-coumaric acids were found the major phenolic metabolites. The method was completely validated, providing a sensitive analysis for bioactive phenolic metabolites detection and showing satisfactory data for all the parameters tested. Moreover, was revealed as an ultra-fast approach allowing the separation of the fifteen bioactive metabolites investigated with high resolution power within 5 min. SN - 1873-3573 UR - https://www.unboundmedicine.com/medline/citation/22063514/A_fast_method_using_a_new_hydrophilic_lipophilic_balanced_sorbent_in_combination_with_ultra_high_performance_liquid_chromatography_for_quantification_of_significant_bioactive_metabolites_in_wines_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0039-9140(11)00707-7 DB - PRIME DP - Unbound Medicine ER -