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Adaptive stress response to menadione-induced oxidative stress in Saccharomyces cerevisiae KNU5377.
J Microbiol. 2011 Oct; 49(5):816-23.JM

Abstract

The molecular mechanisms involved in the ability of yeast cells to adapt and respond to oxidative stress are of great interest to the pharmaceutical, medical, food, and fermentation industries. In this study, we investigated the time-dependent, cellular redox homeostasis ability to adapt to menadione-induced oxidative stress, using biochemical and proteomic approaches in Saccharomyces cerevisiae KNU5377. Time-dependent cell viability was inversely proportional to endogenous amounts of ROS measured by a fluorescence assay with 2',7'-dichlorofluorescin diacetate (DCFHDA), and was hypersensitive when cells were exposed to the compound for 60 min. Morphological changes, protein oxidation and lipid peroxidation were also observed. To overcome the unfavorable conditions due to the presence of menadione, yeast cells activated a variety of cell rescue proteins including antioxidant enzymes, molecular chaperones, energy-generating metabolic enzymes, and antioxidant molecules such as trehalose. Thus, these results show that menadione causes ROS generation and high accumulation of cellular ROS levels, which affects cell viability and cell morphology and there is a correlation between resistance to menadione and the high induction of cell rescue proteins after cells enter into this physiological state, which provides a clue about the complex and dynamic stress response in yeast cells.

Authors+Show Affiliations

Department of Microbiology, Kyungpook National University, Daegu, 702-701, Republic of Korea. 92kis@hanmail.netNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22068500

Citation

Kim, Il-Sup, et al. "Adaptive Stress Response to Menadione-induced Oxidative Stress in Saccharomyces Cerevisiae KNU5377." Journal of Microbiology (Seoul, Korea), vol. 49, no. 5, 2011, pp. 816-23.
Kim IS, Sohn HY, Jin I. Adaptive stress response to menadione-induced oxidative stress in Saccharomyces cerevisiae KNU5377. J Microbiol. 2011;49(5):816-23.
Kim, I. S., Sohn, H. Y., & Jin, I. (2011). Adaptive stress response to menadione-induced oxidative stress in Saccharomyces cerevisiae KNU5377. Journal of Microbiology (Seoul, Korea), 49(5), 816-23. https://doi.org/10.1007/s12275-011-1154-6
Kim IS, Sohn HY, Jin I. Adaptive Stress Response to Menadione-induced Oxidative Stress in Saccharomyces Cerevisiae KNU5377. J Microbiol. 2011;49(5):816-23. PubMed PMID: 22068500.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Adaptive stress response to menadione-induced oxidative stress in Saccharomyces cerevisiae KNU5377. AU - Kim,Il-Sup, AU - Sohn,Ho-Yong, AU - Jin,Ingnyol, Y1 - 2011/11/09/ PY - 2011/03/28/received PY - 2011/05/17/accepted PY - 2011/11/10/entrez PY - 2011/11/10/pubmed PY - 2012/3/6/medline SP - 816 EP - 23 JF - Journal of microbiology (Seoul, Korea) JO - J Microbiol VL - 49 IS - 5 N2 - The molecular mechanisms involved in the ability of yeast cells to adapt and respond to oxidative stress are of great interest to the pharmaceutical, medical, food, and fermentation industries. In this study, we investigated the time-dependent, cellular redox homeostasis ability to adapt to menadione-induced oxidative stress, using biochemical and proteomic approaches in Saccharomyces cerevisiae KNU5377. Time-dependent cell viability was inversely proportional to endogenous amounts of ROS measured by a fluorescence assay with 2',7'-dichlorofluorescin diacetate (DCFHDA), and was hypersensitive when cells were exposed to the compound for 60 min. Morphological changes, protein oxidation and lipid peroxidation were also observed. To overcome the unfavorable conditions due to the presence of menadione, yeast cells activated a variety of cell rescue proteins including antioxidant enzymes, molecular chaperones, energy-generating metabolic enzymes, and antioxidant molecules such as trehalose. Thus, these results show that menadione causes ROS generation and high accumulation of cellular ROS levels, which affects cell viability and cell morphology and there is a correlation between resistance to menadione and the high induction of cell rescue proteins after cells enter into this physiological state, which provides a clue about the complex and dynamic stress response in yeast cells. SN - 1976-3794 UR - https://www.unboundmedicine.com/medline/citation/22068500/Adaptive_stress_response_to_menadione_induced_oxidative_stress_in_Saccharomyces_cerevisiae_KNU5377_ L2 - https://dx.doi.org/10.1007/s12275-011-1154-6 DB - PRIME DP - Unbound Medicine ER -