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The high fermentative metabolism of Kluyveromyces marxianus UFV-3 relies on the increased expression of key lactose metabolic enzymes.
Antonie Van Leeuwenhoek. 2012 Mar; 101(3):541-50.AV

Abstract

The aim of this work was to obtain insights about the factors that determine the lactose fermentative metabolism of Kluyveromyces marxianus UFV-3. K. marxianus UFV-3 and Kluyveromyces lactis JA6 were cultured in a minimal medium containing different lactose concentrations (ranging from 0.25 to 64 mmol l(-1)) under aerobic and hypoxic conditions to evaluate their growth kinetics, gene expression and enzymatic activity. The increase in lactose concentration and the decrease in oxygen level favoured ethanol yield for both yeasts but in K. marxianus UFV-3 the effect was more pronounced. Under hypoxic conditions, the activities of β-galactosidase and pyruvate decarboxylase from K. marxianus UFV-3 were significantly higher than those in K. lactis JA6. The expression of the LAC4 (β-galactosidase), RAG6 (pyruvate decarboxylase), GAL7 (galactose-1-phosphate uridylyltransferase) and GAL10 (epimerase) genes in K. marxianus UFV-3 was higher under hypoxic conditions than under aerobic conditions. The high expression of genes of the Leloir pathway, LAC4 and RAG6, associated with the high activity of β-galactosidase and pyruvate decarboxylase contribute to the high fermentative flux in K. marxianus UFV-3. These data on the fermentative metabolism of K. marxianus UFV-3 will be useful for optimising the conversion of cheese whey lactose to ethanol.

Authors+Show Affiliations

Departamento de Microbiologia, BIOAGRO, Universidade Federal de Viçosa, Av. P H Rolfs s/n, Campus Universitário, Viçosa, MG, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22068918

Citation

Diniz, Raphael H S., et al. "The High Fermentative Metabolism of Kluyveromyces Marxianus UFV-3 Relies On the Increased Expression of Key Lactose Metabolic Enzymes." Antonie Van Leeuwenhoek, vol. 101, no. 3, 2012, pp. 541-50.
Diniz RH, Silveira WB, Fietto LG, et al. The high fermentative metabolism of Kluyveromyces marxianus UFV-3 relies on the increased expression of key lactose metabolic enzymes. Antonie Van Leeuwenhoek. 2012;101(3):541-50.
Diniz, R. H., Silveira, W. B., Fietto, L. G., & Passos, F. M. (2012). The high fermentative metabolism of Kluyveromyces marxianus UFV-3 relies on the increased expression of key lactose metabolic enzymes. Antonie Van Leeuwenhoek, 101(3), 541-50. https://doi.org/10.1007/s10482-011-9668-9
Diniz RH, et al. The High Fermentative Metabolism of Kluyveromyces Marxianus UFV-3 Relies On the Increased Expression of Key Lactose Metabolic Enzymes. Antonie Van Leeuwenhoek. 2012;101(3):541-50. PubMed PMID: 22068918.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The high fermentative metabolism of Kluyveromyces marxianus UFV-3 relies on the increased expression of key lactose metabolic enzymes. AU - Diniz,Raphael H S, AU - Silveira,Wendel B, AU - Fietto,Luciano G, AU - Passos,Flávia M L, Y1 - 2011/11/09/ PY - 2011/07/13/received PY - 2011/10/24/accepted PY - 2011/11/10/entrez PY - 2011/11/10/pubmed PY - 2012/8/15/medline SP - 541 EP - 50 JF - Antonie van Leeuwenhoek JO - Antonie Van Leeuwenhoek VL - 101 IS - 3 N2 - The aim of this work was to obtain insights about the factors that determine the lactose fermentative metabolism of Kluyveromyces marxianus UFV-3. K. marxianus UFV-3 and Kluyveromyces lactis JA6 were cultured in a minimal medium containing different lactose concentrations (ranging from 0.25 to 64 mmol l(-1)) under aerobic and hypoxic conditions to evaluate their growth kinetics, gene expression and enzymatic activity. The increase in lactose concentration and the decrease in oxygen level favoured ethanol yield for both yeasts but in K. marxianus UFV-3 the effect was more pronounced. Under hypoxic conditions, the activities of β-galactosidase and pyruvate decarboxylase from K. marxianus UFV-3 were significantly higher than those in K. lactis JA6. The expression of the LAC4 (β-galactosidase), RAG6 (pyruvate decarboxylase), GAL7 (galactose-1-phosphate uridylyltransferase) and GAL10 (epimerase) genes in K. marxianus UFV-3 was higher under hypoxic conditions than under aerobic conditions. The high expression of genes of the Leloir pathway, LAC4 and RAG6, associated with the high activity of β-galactosidase and pyruvate decarboxylase contribute to the high fermentative flux in K. marxianus UFV-3. These data on the fermentative metabolism of K. marxianus UFV-3 will be useful for optimising the conversion of cheese whey lactose to ethanol. SN - 1572-9699 UR - https://www.unboundmedicine.com/medline/citation/22068918/The_high_fermentative_metabolism_of_Kluyveromyces_marxianus_UFV_3_relies_on_the_increased_expression_of_key_lactose_metabolic_enzymes_ L2 - https://doi.org/10.1007/s10482-011-9668-9 DB - PRIME DP - Unbound Medicine ER -