Tags

Type your tag names separated by a space and hit enter

Trueness in the measurement of haemoglobin: consensus or reference method?
Clin Chem Lab Med. 2011 Nov 10; 50(3):511-4.CC

Abstract

BACKGROUND

For the measurement of haemoglobin a reference method exists: the haemiglobincyanide method. However, a Dutch external quality assessment organization does not use this method in the evaluation of trueness of results. The aim of this work was to assess whether trueness was compromised by the use of a consensus value.

METHODS

Five Cell Dyn Sapphires (Abbott) in three independent locations were used to measure haemoglobin concentration. Results were compared to the reference method (haemiglobincyanide). Patient samples with a distribution over clinically relevant concentrations (Hb 2.5-10.2 mmol/L) were used next to samples from external quality assessment rounds. Passing and Bablok regression analysis and Bland-Altman plots were used to evaluate any systematic deviation.

RESULTS

Results measured on the Cell Dyn Sapphires deviated significantly from the results obtained with the reference method. Remarkably, consensus results from external quality control samples also deviated significantly from the reference method.

CONCLUSIONS

A significant negative bias exists in the measurement of haemoglobin on Cell Dyn Sapphires. Additionally, the consensus value as reported in external quality control assessment also shows an even greater significant negative bias compared to the reference method. As a reference method is available, external quality assessment would benefit from using this method instead of a consensus value to evaluate trueness.

Authors+Show Affiliations

Catharina Hospital Eindhoven, Clinical Laboratory, Eindhoven, The Netherlands. kristel.boonen@cze.nlNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22070349

Citation

Boonen, Kristel J M., et al. "Trueness in the Measurement of Haemoglobin: Consensus or Reference Method?" Clinical Chemistry and Laboratory Medicine, vol. 50, no. 3, 2011, pp. 511-4.
Boonen KJ, Curvers J, Timmerman AA, et al. Trueness in the measurement of haemoglobin: consensus or reference method? Clin Chem Lab Med. 2011;50(3):511-4.
Boonen, K. J., Curvers, J., Timmerman, A. A., Steurs, D., & van de Kerkhof, D. (2011). Trueness in the measurement of haemoglobin: consensus or reference method? Clinical Chemistry and Laboratory Medicine, 50(3), 511-4. https://doi.org/10.1515/CCLM.2011.784
Boonen KJ, et al. Trueness in the Measurement of Haemoglobin: Consensus or Reference Method. Clin Chem Lab Med. 2011 Nov 10;50(3):511-4. PubMed PMID: 22070349.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Trueness in the measurement of haemoglobin: consensus or reference method? AU - Boonen,Kristel J M, AU - Curvers,Joyce, AU - Timmerman,Alex A, AU - Steurs,Diane, AU - van de Kerkhof,Daan, Y1 - 2011/11/10/ PY - 2011/10/07/received PY - 2011/10/18/accepted PY - 2011/11/11/entrez PY - 2011/11/11/pubmed PY - 2012/9/8/medline SP - 511 EP - 4 JF - Clinical chemistry and laboratory medicine JO - Clin. Chem. Lab. Med. VL - 50 IS - 3 N2 - BACKGROUND: For the measurement of haemoglobin a reference method exists: the haemiglobincyanide method. However, a Dutch external quality assessment organization does not use this method in the evaluation of trueness of results. The aim of this work was to assess whether trueness was compromised by the use of a consensus value. METHODS: Five Cell Dyn Sapphires (Abbott) in three independent locations were used to measure haemoglobin concentration. Results were compared to the reference method (haemiglobincyanide). Patient samples with a distribution over clinically relevant concentrations (Hb 2.5-10.2 mmol/L) were used next to samples from external quality assessment rounds. Passing and Bablok regression analysis and Bland-Altman plots were used to evaluate any systematic deviation. RESULTS: Results measured on the Cell Dyn Sapphires deviated significantly from the results obtained with the reference method. Remarkably, consensus results from external quality control samples also deviated significantly from the reference method. CONCLUSIONS: A significant negative bias exists in the measurement of haemoglobin on Cell Dyn Sapphires. Additionally, the consensus value as reported in external quality control assessment also shows an even greater significant negative bias compared to the reference method. As a reference method is available, external quality assessment would benefit from using this method instead of a consensus value to evaluate trueness. SN - 1437-4331 UR - https://www.unboundmedicine.com/medline/citation/22070349/Trueness_in_the_measurement_of_haemoglobin:_consensus_or_reference_method L2 - https://www.degruyter.com/doi/10.1515/CCLM.2011.784 DB - PRIME DP - Unbound Medicine ER -