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Distinct microbial populations exist in the mucosa-associated microbiota of sub-groups of irritable bowel syndrome.
Neurogastroenterol Motil 2012; 24(1):31-9NM

Abstract

BACKGROUND

There is increasing evidence to support a role for the gastrointestinal microbiota in the etiology of irritable bowel syndrome (IBS). Given the evidence of an inflammatory component to IBS, the mucosa-associated microbiota potentially play a key role in its pathogenesis. The objectives were to compare the mucosa-associated microbiota between patients with diarrhea predominant IBS (IBS-D), constipation predominant IBS (IBS-C) and controls using fluorescent in situ hybridization and to correlate specific bacteria groups with individual IBS symptoms.

METHODS

Forty-seven patients with IBS (27 IBS-D and 20 IBS-C) and 26 healthy controls were recruited to the study. Snap-frozen rectal biopsies were taken at colonoscopy and bacterial quantification performed by hybridizing frozen sections with bacterial-group specific oligonucleotide probes.

KEY RESULTS

Patients with IBS had significantly greater numbers of total mucosa-associated bacteria per mm of rectal epithelium than controls [median 218 (IQR - 209) vs 128 (121) P = 0.007], and this was chiefly comprised of bacteroides IBS [69 (67) vs 14 (41) P = 0.001] and Eubacterium rectale-Clostridium coccoides [52 (58) vs 25 (35) P = 0.03]. Analysis of IBS sub-groups demonstrated that bifidobacteria were lower in the IBS-D group than in the IBS-C group and controls [24 (32) vs 54 (88) vs 32 (35) P = 0.011]. Finally, amongst patients with IBS, the maximum number of stools per day negatively correlated with the number of mucosa-associated bifidobacteria (P < 0.001) and lactobacilli (P = 0.002).

CONCLUSIONS & INFERENCES

The mucosa-associated microbiota in patients with IBS is significantly different from healthy controls with increases in bacteroides and clostridia and a reduction in bifidobacteria in patients with IBS-D.

Authors+Show Affiliations

Diet and Gastrointestinal Health, Nutritional Sciences Division, King's College London, London, UK. gareth.parkes@kcl.ac.ukNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22070725

Citation

Parkes, G C., et al. "Distinct Microbial Populations Exist in the Mucosa-associated Microbiota of Sub-groups of Irritable Bowel Syndrome." Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society, vol. 24, no. 1, 2012, pp. 31-9.
Parkes GC, Rayment NB, Hudspith BN, et al. Distinct microbial populations exist in the mucosa-associated microbiota of sub-groups of irritable bowel syndrome. Neurogastroenterol Motil. 2012;24(1):31-9.
Parkes, G. C., Rayment, N. B., Hudspith, B. N., Petrovska, L., Lomer, M. C., Brostoff, J., ... Sanderson, J. D. (2012). Distinct microbial populations exist in the mucosa-associated microbiota of sub-groups of irritable bowel syndrome. Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society, 24(1), pp. 31-9. doi:10.1111/j.1365-2982.2011.01803.x.
Parkes GC, et al. Distinct Microbial Populations Exist in the Mucosa-associated Microbiota of Sub-groups of Irritable Bowel Syndrome. Neurogastroenterol Motil. 2012;24(1):31-9. PubMed PMID: 22070725.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Distinct microbial populations exist in the mucosa-associated microbiota of sub-groups of irritable bowel syndrome. AU - Parkes,G C, AU - Rayment,N B, AU - Hudspith,B N, AU - Petrovska,L, AU - Lomer,M C, AU - Brostoff,J, AU - Whelan,K, AU - Sanderson,J D, Y1 - 2011/11/09/ PY - 2011/11/11/entrez PY - 2011/11/11/pubmed PY - 2012/4/12/medline SP - 31 EP - 9 JF - Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society JO - Neurogastroenterol. Motil. VL - 24 IS - 1 N2 - BACKGROUND: There is increasing evidence to support a role for the gastrointestinal microbiota in the etiology of irritable bowel syndrome (IBS). Given the evidence of an inflammatory component to IBS, the mucosa-associated microbiota potentially play a key role in its pathogenesis. The objectives were to compare the mucosa-associated microbiota between patients with diarrhea predominant IBS (IBS-D), constipation predominant IBS (IBS-C) and controls using fluorescent in situ hybridization and to correlate specific bacteria groups with individual IBS symptoms. METHODS: Forty-seven patients with IBS (27 IBS-D and 20 IBS-C) and 26 healthy controls were recruited to the study. Snap-frozen rectal biopsies were taken at colonoscopy and bacterial quantification performed by hybridizing frozen sections with bacterial-group specific oligonucleotide probes. KEY RESULTS: Patients with IBS had significantly greater numbers of total mucosa-associated bacteria per mm of rectal epithelium than controls [median 218 (IQR - 209) vs 128 (121) P = 0.007], and this was chiefly comprised of bacteroides IBS [69 (67) vs 14 (41) P = 0.001] and Eubacterium rectale-Clostridium coccoides [52 (58) vs 25 (35) P = 0.03]. Analysis of IBS sub-groups demonstrated that bifidobacteria were lower in the IBS-D group than in the IBS-C group and controls [24 (32) vs 54 (88) vs 32 (35) P = 0.011]. Finally, amongst patients with IBS, the maximum number of stools per day negatively correlated with the number of mucosa-associated bifidobacteria (P < 0.001) and lactobacilli (P = 0.002). CONCLUSIONS & INFERENCES: The mucosa-associated microbiota in patients with IBS is significantly different from healthy controls with increases in bacteroides and clostridia and a reduction in bifidobacteria in patients with IBS-D. SN - 1365-2982 UR - https://www.unboundmedicine.com/medline/citation/22070725/Distinct_microbial_populations_exist_in_the_mucosa_associated_microbiota_of_sub_groups_of_irritable_bowel_syndrome_ L2 - https://doi.org/10.1111/j.1365-2982.2011.01803.x DB - PRIME DP - Unbound Medicine ER -