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Simultaneous quantification of polymethoxylated flavones and coumarins in Fructus aurantii and Fructus aurantii immaturus using HPLC-ESI-MS/MS.
J Pharm Biomed Anal. 2012 Feb 05; 59:90-5.JP

Abstract

The major lipid-soluble constituents in Fructus aurantii (zhiqiao) and Fructus aurantii immaturus (zhishi) are polymethoxylated flavones (PMFs) and coumarins. In the present study, a high-performance liquid chromatography with electrospray ionization tandem mass spectrometry method was developed to quantify PMFs (nobiletin, tangeretin, 5-hydroxy-6,7,8,4'-tetramethoxyflavone, and natsudaidai) and coumarins (marmin, meranzin hydrate, and auraptene) simultaneously. PMFs and coumarins were detected by electrospray ionization tandem mass spectrometry in positive ion mode and quantified with multiple reaction monitor. Samples were separated on a Diamonsil C₁₈ (150 mm × 4.6 mm, 5 μm) column using acetonitrile and formic acid-water solution as a mobile phase in gradient mode with a flow rate at 0.5 mL/min. All calibration curves showed good linearity (r² > 0.9977) within the test ranges. Variations of the intraday and interday precisions were less than 4.07%. The recoveries of the components were within the range of 95.79%-105.04% and the relative standard deviations were less than 3.82%. The method developed was validated with acceptable accuracy, precision, and extraction recoveries and can be applied for the identification and quantification of four PMFs and three coumarins in citrus herbs.

Authors+Show Affiliations

The Key Laboratory of Modern Preparation of TCM of Ministry of Education, Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi Province 330004, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22071443

Citation

Chen, Hai-Fang, et al. "Simultaneous Quantification of Polymethoxylated Flavones and Coumarins in Fructus Aurantii and Fructus Aurantii Immaturus Using HPLC-ESI-MS/MS." Journal of Pharmaceutical and Biomedical Analysis, vol. 59, 2012, pp. 90-5.
Chen HF, Zhang WG, Yuan JB, et al. Simultaneous quantification of polymethoxylated flavones and coumarins in Fructus aurantii and Fructus aurantii immaturus using HPLC-ESI-MS/MS. J Pharm Biomed Anal. 2012;59:90-5.
Chen, H. F., Zhang, W. G., Yuan, J. B., Li, Y. G., Yang, S. L., & Yang, W. L. (2012). Simultaneous quantification of polymethoxylated flavones and coumarins in Fructus aurantii and Fructus aurantii immaturus using HPLC-ESI-MS/MS. Journal of Pharmaceutical and Biomedical Analysis, 59, 90-5. https://doi.org/10.1016/j.jpba.2011.10.013
Chen HF, et al. Simultaneous Quantification of Polymethoxylated Flavones and Coumarins in Fructus Aurantii and Fructus Aurantii Immaturus Using HPLC-ESI-MS/MS. J Pharm Biomed Anal. 2012 Feb 5;59:90-5. PubMed PMID: 22071443.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous quantification of polymethoxylated flavones and coumarins in Fructus aurantii and Fructus aurantii immaturus using HPLC-ESI-MS/MS. AU - Chen,Hai-Fang, AU - Zhang,Wu-Gang, AU - Yuan,Jin-Bin, AU - Li,Yan-Gang, AU - Yang,Shi-Lin, AU - Yang,Wu-Liang, Y1 - 2011/10/20/ PY - 2011/05/10/received PY - 2011/10/13/revised PY - 2011/10/14/accepted PY - 2011/11/11/entrez PY - 2011/11/11/pubmed PY - 2012/5/30/medline SP - 90 EP - 5 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 59 N2 - The major lipid-soluble constituents in Fructus aurantii (zhiqiao) and Fructus aurantii immaturus (zhishi) are polymethoxylated flavones (PMFs) and coumarins. In the present study, a high-performance liquid chromatography with electrospray ionization tandem mass spectrometry method was developed to quantify PMFs (nobiletin, tangeretin, 5-hydroxy-6,7,8,4'-tetramethoxyflavone, and natsudaidai) and coumarins (marmin, meranzin hydrate, and auraptene) simultaneously. PMFs and coumarins were detected by electrospray ionization tandem mass spectrometry in positive ion mode and quantified with multiple reaction monitor. Samples were separated on a Diamonsil C₁₈ (150 mm × 4.6 mm, 5 μm) column using acetonitrile and formic acid-water solution as a mobile phase in gradient mode with a flow rate at 0.5 mL/min. All calibration curves showed good linearity (r² > 0.9977) within the test ranges. Variations of the intraday and interday precisions were less than 4.07%. The recoveries of the components were within the range of 95.79%-105.04% and the relative standard deviations were less than 3.82%. The method developed was validated with acceptable accuracy, precision, and extraction recoveries and can be applied for the identification and quantification of four PMFs and three coumarins in citrus herbs. SN - 1873-264X UR - https://www.unboundmedicine.com/medline/citation/22071443/Simultaneous_quantification_of_polymethoxylated_flavones_and_coumarins_in_Fructus_aurantii_and_Fructus_aurantii_immaturus_using_HPLC_ESI_MS/MS_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0731-7085(11)00589-9 DB - PRIME DP - Unbound Medicine ER -