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Simultaneous determination of 23 amino acids and 7 biogenic amines in fermented food samples by liquid chromatography/quadrupole time-of-flight mass spectrometry.
J Chromatogr A. 2011 Dec 23; 1218(51):9174-82.JC

Abstract

A novel liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (LC-Q-TOFMS) method was developed for the simultaneous determination of 23 amino acids and 7 biogenic amines in food samples. These analytes were pre-column derivatized with dansyl chloride and then separated in an Acquity column (1.7 μm; 2.1 mm × 100 mm). The separation of 31 compounds including an internal standard was achieved within 25 min at a flow rate of 0.2 mL/min. The method linearity for each amino acid and biogenic amine had a relatively wide range with r(2)>0.99. The intra- and inter-day precision, expressed as relative standard deviation (RSD), ranged from 1.1 to 4.6% and from 2.0 to 11.2%, respectively. The limit of detection was between 0.005 and 0.4 μg/mL. With a simple dilution, recoveries of around 80-120% were obtained for most of the compounds. No significant matrix effect was observed, and the developed method was successfully applied to the analysis of amino acids and biogenic amines in beer, cheese and sausage samples.

Authors+Show Affiliations

College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul 151-742, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22074649

Citation

Jia, Shaodong, et al. "Simultaneous Determination of 23 Amino Acids and 7 Biogenic Amines in Fermented Food Samples By Liquid Chromatography/quadrupole Time-of-flight Mass Spectrometry." Journal of Chromatography. A, vol. 1218, no. 51, 2011, pp. 9174-82.
Jia S, Kang YP, Park JH, et al. Simultaneous determination of 23 amino acids and 7 biogenic amines in fermented food samples by liquid chromatography/quadrupole time-of-flight mass spectrometry. J Chromatogr A. 2011;1218(51):9174-82.
Jia, S., Kang, Y. P., Park, J. H., Lee, J., & Kwon, S. W. (2011). Simultaneous determination of 23 amino acids and 7 biogenic amines in fermented food samples by liquid chromatography/quadrupole time-of-flight mass spectrometry. Journal of Chromatography. A, 1218(51), 9174-82. https://doi.org/10.1016/j.chroma.2011.10.040
Jia S, et al. Simultaneous Determination of 23 Amino Acids and 7 Biogenic Amines in Fermented Food Samples By Liquid Chromatography/quadrupole Time-of-flight Mass Spectrometry. J Chromatogr A. 2011 Dec 23;1218(51):9174-82. PubMed PMID: 22074649.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous determination of 23 amino acids and 7 biogenic amines in fermented food samples by liquid chromatography/quadrupole time-of-flight mass spectrometry. AU - Jia,Shaodong, AU - Kang,Yun Pyo, AU - Park,Jeong Hill, AU - Lee,Jeongmi, AU - Kwon,Sung Won, Y1 - 2011/10/21/ PY - 2011/06/03/received PY - 2011/10/10/revised PY - 2011/10/17/accepted PY - 2011/11/15/entrez PY - 2011/11/15/pubmed PY - 2012/2/9/medline SP - 9174 EP - 82 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1218 IS - 51 N2 - A novel liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (LC-Q-TOFMS) method was developed for the simultaneous determination of 23 amino acids and 7 biogenic amines in food samples. These analytes were pre-column derivatized with dansyl chloride and then separated in an Acquity column (1.7 μm; 2.1 mm × 100 mm). The separation of 31 compounds including an internal standard was achieved within 25 min at a flow rate of 0.2 mL/min. The method linearity for each amino acid and biogenic amine had a relatively wide range with r(2)>0.99. The intra- and inter-day precision, expressed as relative standard deviation (RSD), ranged from 1.1 to 4.6% and from 2.0 to 11.2%, respectively. The limit of detection was between 0.005 and 0.4 μg/mL. With a simple dilution, recoveries of around 80-120% were obtained for most of the compounds. No significant matrix effect was observed, and the developed method was successfully applied to the analysis of amino acids and biogenic amines in beer, cheese and sausage samples. SN - 1873-3778 UR - https://www.unboundmedicine.com/medline/citation/22074649/Simultaneous_determination_of_23_amino_acids_and_7_biogenic_amines_in_fermented_food_samples_by_liquid_chromatography/quadrupole_time_of_flight_mass_spectrometry_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(11)01562-7 DB - PRIME DP - Unbound Medicine ER -