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Monoclonal antibody 12D5 inhibits eosinophil infiltration in the brain of Angiostrongylus cantonensis-infected BALB/c mice.
Acta Trop. 2012 Feb; 121(2):118-24.AT

Abstract

Each of BALB/c mice was infected with 50 Angiostrongylus cantonensis larvae. One group of mice received an intraperitoneal injection of 50 μg 12D5 monoclonal antibody (mAb) against a 98 kDa antigen of adult worms at 10 days post-infection (dpi), with a booster injection of 25 μg at 12 dpi. Five mice from each group were sacrificed at 14 dpi for pathological examination and RNA extraction. The infiltration of eosinophils and severity of eosinophilic meningitis were reduced in 12D5 mAb-treated mice compared with the infected mice without 12D5 treatment. The levels of eotaxin mRNA expression in spleen significantly increased and the expression of the Th2-type cytokine IL-5 significantly decreased. However, the expression of IL-4 was not changed. 12D5 mAb can observably enhance the survival rate of infected mice and reduce symptoms of angiostrongyliasis. A. cantonensis infection is a major cause of eosinophilic meningoencephalitis. The results of this study could be helpful for the development of treatment of human angiostrongylosis.

Authors+Show Affiliations

Shenzhen Center for Disease Control and Prevention, Shenzhen, Guangdong Province, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22074686

Citation

Li, Jun-Jian, et al. "Monoclonal Antibody 12D5 Inhibits Eosinophil Infiltration in the Brain of Angiostrongylus Cantonensis-infected BALB/c Mice." Acta Tropica, vol. 121, no. 2, 2012, pp. 118-24.
Li JJ, Zhang RL, Fu YC, et al. Monoclonal antibody 12D5 inhibits eosinophil infiltration in the brain of Angiostrongylus cantonensis-infected BALB/c mice. Acta Trop. 2012;121(2):118-24.
Li, J. J., Zhang, R. L., Fu, Y. C., Wu, W. P., Chen, M. X., Geng, Y. J., Huang, D. N., Ai, L., Yang, F., & Hu, Z. (2012). Monoclonal antibody 12D5 inhibits eosinophil infiltration in the brain of Angiostrongylus cantonensis-infected BALB/c mice. Acta Tropica, 121(2), 118-24. https://doi.org/10.1016/j.actatropica.2011.10.013
Li JJ, et al. Monoclonal Antibody 12D5 Inhibits Eosinophil Infiltration in the Brain of Angiostrongylus Cantonensis-infected BALB/c Mice. Acta Trop. 2012;121(2):118-24. PubMed PMID: 22074686.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Monoclonal antibody 12D5 inhibits eosinophil infiltration in the brain of Angiostrongylus cantonensis-infected BALB/c mice. AU - Li,Jun-Jian, AU - Zhang,Ren-Li, AU - Fu,Yu-Cai, AU - Wu,Wei-ping, AU - Chen,Mu-Xin, AU - Geng,Yi-Jie, AU - Huang,Da-Na, AU - Ai,Lin, AU - Yang,Fan, AU - Hu,Zhong, Y1 - 2011/11/02/ PY - 2011/04/25/received PY - 2011/10/08/revised PY - 2011/10/23/accepted PY - 2011/11/15/entrez PY - 2011/11/15/pubmed PY - 2014/1/22/medline SP - 118 EP - 24 JF - Acta tropica JO - Acta Trop VL - 121 IS - 2 N2 - Each of BALB/c mice was infected with 50 Angiostrongylus cantonensis larvae. One group of mice received an intraperitoneal injection of 50 μg 12D5 monoclonal antibody (mAb) against a 98 kDa antigen of adult worms at 10 days post-infection (dpi), with a booster injection of 25 μg at 12 dpi. Five mice from each group were sacrificed at 14 dpi for pathological examination and RNA extraction. The infiltration of eosinophils and severity of eosinophilic meningitis were reduced in 12D5 mAb-treated mice compared with the infected mice without 12D5 treatment. The levels of eotaxin mRNA expression in spleen significantly increased and the expression of the Th2-type cytokine IL-5 significantly decreased. However, the expression of IL-4 was not changed. 12D5 mAb can observably enhance the survival rate of infected mice and reduce symptoms of angiostrongyliasis. A. cantonensis infection is a major cause of eosinophilic meningoencephalitis. The results of this study could be helpful for the development of treatment of human angiostrongylosis. SN - 1873-6254 UR - https://www.unboundmedicine.com/medline/citation/22074686/Monoclonal_antibody_12D5_inhibits_eosinophil_infiltration_in_the_brain_of_Angiostrongylus_cantonensis_infected_BALB/c_mice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0001-706X(11)00309-3 DB - PRIME DP - Unbound Medicine ER -