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Alkamides activate jasmonic acid biosynthesis and signaling pathways and confer resistance to Botrytis cinerea in Arabidopsis thaliana.
PLoS One. 2011; 6(11):e27251.Plos

Abstract

Alkamides are fatty acid amides of wide distribution in plants, structurally related to N-acyl-L-homoserine lactones (AHLs) from Gram-negative bacteria and to N- acylethanolamines (NAEs) from plants and mammals. Global analysis of gene expression changes in Arabidopsis thaliana in response to N-isobutyl decanamide, the most highly active alkamide identified to date, revealed an overrepresentation of defense-responsive transcriptional networks. In particular, genes encoding enzymes for jasmonic acid (JA) biosynthesis increased their expression, which occurred in parallel with JA, nitric oxide (NO) and H₂O₂ accumulation. The activity of the alkamide to confer resistance against the necrotizing fungus Botrytis cinerea was tested by inoculating Arabidopsis detached leaves with conidiospores and evaluating disease symptoms and fungal proliferation. N-isobutyl decanamide application significantly reduced necrosis caused by the pathogen and inhibited fungal proliferation. Arabidopsis mutants jar1 and coi1 altered in JA signaling and a MAP kinase mutant (mpk6), unlike salicylic acid- (SA) related mutant eds16/sid2-1, were unable to defend from fungal attack even when N-isobutyl decanamide was supplied, indicating that alkamides could modulate some necrotrophic-associated defense responses through JA-dependent and MPK6-regulated signaling pathways. Our results suggest a role of alkamides in plant immunity induction.

Authors+Show Affiliations

Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Morelia, Michoacán, México.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22076141

Citation

Méndez-Bravo, Alfonso, et al. "Alkamides Activate Jasmonic Acid Biosynthesis and Signaling Pathways and Confer Resistance to Botrytis Cinerea in Arabidopsis Thaliana." PloS One, vol. 6, no. 11, 2011, pp. e27251.
Méndez-Bravo A, Calderón-Vázquez C, Ibarra-Laclette E, et al. Alkamides activate jasmonic acid biosynthesis and signaling pathways and confer resistance to Botrytis cinerea in Arabidopsis thaliana. PLoS One. 2011;6(11):e27251.
Méndez-Bravo, A., Calderón-Vázquez, C., Ibarra-Laclette, E., Raya-González, J., Ramírez-Chávez, E., Molina-Torres, J., Guevara-García, A. A., López-Bucio, J., & Herrera-Estrella, L. (2011). Alkamides activate jasmonic acid biosynthesis and signaling pathways and confer resistance to Botrytis cinerea in Arabidopsis thaliana. PloS One, 6(11), e27251. https://doi.org/10.1371/journal.pone.0027251
Méndez-Bravo A, et al. Alkamides Activate Jasmonic Acid Biosynthesis and Signaling Pathways and Confer Resistance to Botrytis Cinerea in Arabidopsis Thaliana. PLoS One. 2011;6(11):e27251. PubMed PMID: 22076141.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Alkamides activate jasmonic acid biosynthesis and signaling pathways and confer resistance to Botrytis cinerea in Arabidopsis thaliana. AU - Méndez-Bravo,Alfonso, AU - Calderón-Vázquez,Carlos, AU - Ibarra-Laclette,Enrique, AU - Raya-González,Javier, AU - Ramírez-Chávez,Enrique, AU - Molina-Torres,Jorge, AU - Guevara-García,Angel A, AU - López-Bucio,José, AU - Herrera-Estrella,Luis, Y1 - 2011/11/04/ PY - 2011/04/13/received PY - 2011/10/12/accepted PY - 2011/11/15/entrez PY - 2011/11/15/pubmed PY - 2012/3/16/medline SP - e27251 EP - e27251 JF - PloS one JO - PLoS One VL - 6 IS - 11 N2 - Alkamides are fatty acid amides of wide distribution in plants, structurally related to N-acyl-L-homoserine lactones (AHLs) from Gram-negative bacteria and to N- acylethanolamines (NAEs) from plants and mammals. Global analysis of gene expression changes in Arabidopsis thaliana in response to N-isobutyl decanamide, the most highly active alkamide identified to date, revealed an overrepresentation of defense-responsive transcriptional networks. In particular, genes encoding enzymes for jasmonic acid (JA) biosynthesis increased their expression, which occurred in parallel with JA, nitric oxide (NO) and H₂O₂ accumulation. The activity of the alkamide to confer resistance against the necrotizing fungus Botrytis cinerea was tested by inoculating Arabidopsis detached leaves with conidiospores and evaluating disease symptoms and fungal proliferation. N-isobutyl decanamide application significantly reduced necrosis caused by the pathogen and inhibited fungal proliferation. Arabidopsis mutants jar1 and coi1 altered in JA signaling and a MAP kinase mutant (mpk6), unlike salicylic acid- (SA) related mutant eds16/sid2-1, were unable to defend from fungal attack even when N-isobutyl decanamide was supplied, indicating that alkamides could modulate some necrotrophic-associated defense responses through JA-dependent and MPK6-regulated signaling pathways. Our results suggest a role of alkamides in plant immunity induction. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/22076141/Alkamides_activate_jasmonic_acid_biosynthesis_and_signaling_pathways_and_confer_resistance_to_Botrytis_cinerea_in_Arabidopsis_thaliana_ L2 - https://dx.plos.org/10.1371/journal.pone.0027251 DB - PRIME DP - Unbound Medicine ER -