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Flow cytometry counting of bronchoalveolar lavage leukocytes with a new profile of monoclonal antibodies combination.
Cytometry B Clin Cytom. 2012 Mar; 82(2):61-6.CB

Abstract

BACKGROUND

Differential cell count in bronchoalveolar lavage (BAL) has an important role in the diagnosis of pulmonary diseases. Optical microscopy method is usually chosen to identify BAL leukocyte populations despite its technical limitations. As there are no guidelines to make this analysis by flow cytometry (FCM), we propose a new monoclonal antibodies combination for this analysis.

METHODS

Thirty-four BAL samples were stained with the monoclonal antibodies combination CD15/CD16/CD45/HLA-DR and analyzed in a 2-laser cytometer (FACSCalibur). The results were compared with those obtained by optical microscopy.

RESULTS

Both methods showed a good correlation, but FCM overestimates lymphocyte population and conversely underestimates alveolar macrophage population.

CONCLUSIONS

The proposed monoclonal antibodies combination is effective and reliable to identify leukocyte populations in BAL.

Authors+Show Affiliations

Immunology Department, Hospital Universitario Central de Asturias, Oviedo, Spain. lourdes.tricas@sespa.princast.esNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22076931

Citation

Tricas, Lourdes, et al. "Flow Cytometry Counting of Bronchoalveolar Lavage Leukocytes With a New Profile of Monoclonal Antibodies Combination." Cytometry. Part B, Clinical Cytometry, vol. 82, no. 2, 2012, pp. 61-6.
Tricas L, Echeverría A, Blanco MA, et al. Flow cytometry counting of bronchoalveolar lavage leukocytes with a new profile of monoclonal antibodies combination. Cytometry B Clin Cytom. 2012;82(2):61-6.
Tricas, L., Echeverría, A., Blanco, M. A., Menéndez, M., & Belda, J. (2012). Flow cytometry counting of bronchoalveolar lavage leukocytes with a new profile of monoclonal antibodies combination. Cytometry. Part B, Clinical Cytometry, 82(2), 61-6. https://doi.org/10.1002/cyto.b.20630
Tricas L, et al. Flow Cytometry Counting of Bronchoalveolar Lavage Leukocytes With a New Profile of Monoclonal Antibodies Combination. Cytometry B Clin Cytom. 2012;82(2):61-6. PubMed PMID: 22076931.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Flow cytometry counting of bronchoalveolar lavage leukocytes with a new profile of monoclonal antibodies combination. AU - Tricas,Lourdes, AU - Echeverría,Ainara, AU - Blanco,Miguel A, AU - Menéndez,Manuel, AU - Belda,José, Y1 - 2011/11/10/ PY - 2011/01/26/received PY - 2011/08/25/revised PY - 2011/09/28/accepted PY - 2011/11/15/entrez PY - 2011/11/15/pubmed PY - 2012/8/31/medline SP - 61 EP - 6 JF - Cytometry. Part B, Clinical cytometry JO - Cytometry B Clin Cytom VL - 82 IS - 2 N2 - BACKGROUND: Differential cell count in bronchoalveolar lavage (BAL) has an important role in the diagnosis of pulmonary diseases. Optical microscopy method is usually chosen to identify BAL leukocyte populations despite its technical limitations. As there are no guidelines to make this analysis by flow cytometry (FCM), we propose a new monoclonal antibodies combination for this analysis. METHODS: Thirty-four BAL samples were stained with the monoclonal antibodies combination CD15/CD16/CD45/HLA-DR and analyzed in a 2-laser cytometer (FACSCalibur). The results were compared with those obtained by optical microscopy. RESULTS: Both methods showed a good correlation, but FCM overestimates lymphocyte population and conversely underestimates alveolar macrophage population. CONCLUSIONS: The proposed monoclonal antibodies combination is effective and reliable to identify leukocyte populations in BAL. SN - 1552-4957 UR - https://www.unboundmedicine.com/medline/citation/22076931/Flow_cytometry_counting_of_bronchoalveolar_lavage_leukocytes_with_a_new_profile_of_monoclonal_antibodies_combination_ L2 - https://doi.org/10.1002/cyto.b.20630 DB - PRIME DP - Unbound Medicine ER -