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Bee venom protects SH-SY5Y human neuroblastoma cells from 1-methyl-4-phenylpyridinium-induced apoptotic cell death.
Brain Res. 2012 Jan 06; 1429:106-15.BR

Abstract

Parkinson's disease (PD) is a progressive neurodegenerative disorder characterized by progressive selective loss of dopaminergic neurons in the substantia nigra. Recently, bee venom was reported to protect dopaminergic neurons in the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine induced mice PD model, however, the underlying mechanism is not fully understood. The objective of the present study is to investigate the neuroprotective mechanism of bee venom against Parkinsonian toxin, 1-methyl-4-phenylpyridine (MPP(+)), in SH-SY5Y human neuroblastoma cells. Our results revealed that bee venom pretreatment (1-100 ng/ml) increased the cell viability and decreased apoptosis assessed by DNA fragmentation and caspase-3 activity assays in MPP(+)-induced cytotoxicity in SH-SY5Y cells. Bee venom increased the anti-apoptotic Bcl-2 expression and decreased the pro-apoptotic Bax, cleaved PARP expressions. In addition, bee venom prevented the MPP(+)-induced suppression of Akt phosphorylation, and the neuroprotective effect of bee venom against MPP(+)-induced cytotoxicity was inhibited by a phosphatidylinositol 3-kinase (PI3K) inhibitor, LY294002. These results suggest that the anti-apoptotic effect of bee venom is mediated by the cell survival signaling, the PI3K/Akt pathway. These results provide new evidence for elucidating the mechanism of neuroprotection of bee venom against PD.

Authors+Show Affiliations

Studies of Translational Acupuncture Research (STAR), Acupuncture & Meridian Science Research Center (AMSRC), Kyung Hee University, 1 Hoegi-dong, Dongdaemun-gu, Seoul 130-701, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22078207

Citation

Doo, Ah-Reum, et al. "Bee Venom Protects SH-SY5Y Human Neuroblastoma Cells From 1-methyl-4-phenylpyridinium-induced Apoptotic Cell Death." Brain Research, vol. 1429, 2012, pp. 106-15.
Doo AR, Kim SN, Kim ST, et al. Bee venom protects SH-SY5Y human neuroblastoma cells from 1-methyl-4-phenylpyridinium-induced apoptotic cell death. Brain Res. 2012;1429:106-15.
Doo, A. R., Kim, S. N., Kim, S. T., Park, J. Y., Chung, S. H., Choe, B. Y., Chae, Y., Lee, H., Yin, C. S., & Park, H. J. (2012). Bee venom protects SH-SY5Y human neuroblastoma cells from 1-methyl-4-phenylpyridinium-induced apoptotic cell death. Brain Research, 1429, 106-15. https://doi.org/10.1016/j.brainres.2011.10.003
Doo AR, et al. Bee Venom Protects SH-SY5Y Human Neuroblastoma Cells From 1-methyl-4-phenylpyridinium-induced Apoptotic Cell Death. Brain Res. 2012 Jan 6;1429:106-15. PubMed PMID: 22078207.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Bee venom protects SH-SY5Y human neuroblastoma cells from 1-methyl-4-phenylpyridinium-induced apoptotic cell death. AU - Doo,Ah-Reum, AU - Kim,Seung-Nam, AU - Kim,Seung-Tae, AU - Park,Ji-Yeun, AU - Chung,Sung-Hyun, AU - Choe,Bo-Young, AU - Chae,Younbyoung, AU - Lee,Hyejung, AU - Yin,Chang-Shik, AU - Park,Hi-Joon, Y1 - 2011/10/06/ PY - 2011/03/29/received PY - 2011/09/23/revised PY - 2011/10/01/accepted PY - 2011/11/15/entrez PY - 2011/11/15/pubmed PY - 2012/3/21/medline SP - 106 EP - 15 JF - Brain research JO - Brain Res VL - 1429 N2 - Parkinson's disease (PD) is a progressive neurodegenerative disorder characterized by progressive selective loss of dopaminergic neurons in the substantia nigra. Recently, bee venom was reported to protect dopaminergic neurons in the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine induced mice PD model, however, the underlying mechanism is not fully understood. The objective of the present study is to investigate the neuroprotective mechanism of bee venom against Parkinsonian toxin, 1-methyl-4-phenylpyridine (MPP(+)), in SH-SY5Y human neuroblastoma cells. Our results revealed that bee venom pretreatment (1-100 ng/ml) increased the cell viability and decreased apoptosis assessed by DNA fragmentation and caspase-3 activity assays in MPP(+)-induced cytotoxicity in SH-SY5Y cells. Bee venom increased the anti-apoptotic Bcl-2 expression and decreased the pro-apoptotic Bax, cleaved PARP expressions. In addition, bee venom prevented the MPP(+)-induced suppression of Akt phosphorylation, and the neuroprotective effect of bee venom against MPP(+)-induced cytotoxicity was inhibited by a phosphatidylinositol 3-kinase (PI3K) inhibitor, LY294002. These results suggest that the anti-apoptotic effect of bee venom is mediated by the cell survival signaling, the PI3K/Akt pathway. These results provide new evidence for elucidating the mechanism of neuroprotection of bee venom against PD. SN - 1872-6240 UR - https://www.unboundmedicine.com/medline/citation/22078207/Bee_venom_protects_SH_SY5Y_human_neuroblastoma_cells_from_1_methyl_4_phenylpyridinium_induced_apoptotic_cell_death_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-8993(11)01839-7 DB - PRIME DP - Unbound Medicine ER -