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In mammalian muscle, SIRT3 is present in mitochondria and not in the nucleus; and SIRT3 is upregulated by chronic muscle contraction in an adenosine monophosphate-activated protein kinase-independent manner.
Metabolism. 2012 May; 61(5):733-41.M

Abstract

In selected cell lines, it appears (a) that metabolic stressors induce the translocation of SIRT3 from the nucleus to mitochondria and (b) that SIRT3 may contribute to the regulation of mitochondrial biogenesis and/or fatty acid utilization. We have examined in mammalian muscle (1) the association between SIRT3 protein content and muscle oxidative capacity and mitochondrial fatty acid oxidation, (2) the subcellular location of SIRT3, (3) whether exercise induces the translocation of SIRT3 from the nucleus to the mitochondria, and (4) the response of SIRT3 protein to stressors known to induce mitochondrial biogenesis (chronic muscle stimulation and 5-aminoimidazole-4-carboxamide-1-β-d-ribofuranoside administration). SIRT3 protein displayed hierarchical expression based on oxidative potential of muscle tissues (heart >> red >> white). In contrast to studies in some cell lines, metabolic stress (exercise) did not induce the translocation of SIRT3 from the nucleus to mitochondria, as SIRT3 was only present in subsarcolemmal (SS) and intermyofibrillar (IMF) mitochondria, not in the nucleus. Chronic stimulation increased muscle mitochondrial content and SIRT3 protein in SS (+33%) and IMF (+27%) mitochondria (P < .05). In contrast, chronic 5-aminoimidazole-4-carboxamide-1-β-d-ribofuranoside administration, while inducing mitochondrial biogenesis, did not alter SS or IMF mitochondrial SIRT3 protein content. These studies have shown that, in muscle, SIRT3 (a) scales with muscle oxidative capacity and with enzymes regulating fatty acid oxidation, (b) in resting muscle is localized to SS and IMF mitochondria and not nuclei, (c) in contracting muscle is not acutely translocated to mitochondria, and (d) is upregulated with chronic stimulation in an adenosine monophosphate-activated protein kinase-independent manner.

Authors+Show Affiliations

School of Kinesiology, Queen's University, Kingston, Ontario, Canada K7L 3N6. gurdb@queensu.caNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22078938

Citation

Gurd, Brendon J., et al. "In Mammalian Muscle, SIRT3 Is Present in Mitochondria and Not in the Nucleus; and SIRT3 Is Upregulated By Chronic Muscle Contraction in an Adenosine Monophosphate-activated Protein Kinase-independent Manner." Metabolism: Clinical and Experimental, vol. 61, no. 5, 2012, pp. 733-41.
Gurd BJ, Holloway GP, Yoshida Y, et al. In mammalian muscle, SIRT3 is present in mitochondria and not in the nucleus; and SIRT3 is upregulated by chronic muscle contraction in an adenosine monophosphate-activated protein kinase-independent manner. Metab Clin Exp. 2012;61(5):733-41.
Gurd, B. J., Holloway, G. P., Yoshida, Y., & Bonen, A. (2012). In mammalian muscle, SIRT3 is present in mitochondria and not in the nucleus; and SIRT3 is upregulated by chronic muscle contraction in an adenosine monophosphate-activated protein kinase-independent manner. Metabolism: Clinical and Experimental, 61(5), 733-41. https://doi.org/10.1016/j.metabol.2011.09.016
Gurd BJ, et al. In Mammalian Muscle, SIRT3 Is Present in Mitochondria and Not in the Nucleus; and SIRT3 Is Upregulated By Chronic Muscle Contraction in an Adenosine Monophosphate-activated Protein Kinase-independent Manner. Metab Clin Exp. 2012;61(5):733-41. PubMed PMID: 22078938.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In mammalian muscle, SIRT3 is present in mitochondria and not in the nucleus; and SIRT3 is upregulated by chronic muscle contraction in an adenosine monophosphate-activated protein kinase-independent manner. AU - Gurd,Brendon J, AU - Holloway,Graham P, AU - Yoshida,Yuko, AU - Bonen,Arend, Y1 - 2011/11/09/ PY - 2011/03/11/received PY - 2011/09/19/revised PY - 2011/09/28/accepted PY - 2011/11/15/entrez PY - 2011/11/15/pubmed PY - 2012/6/21/medline SP - 733 EP - 41 JF - Metabolism: clinical and experimental JO - Metab. Clin. Exp. VL - 61 IS - 5 N2 - In selected cell lines, it appears (a) that metabolic stressors induce the translocation of SIRT3 from the nucleus to mitochondria and (b) that SIRT3 may contribute to the regulation of mitochondrial biogenesis and/or fatty acid utilization. We have examined in mammalian muscle (1) the association between SIRT3 protein content and muscle oxidative capacity and mitochondrial fatty acid oxidation, (2) the subcellular location of SIRT3, (3) whether exercise induces the translocation of SIRT3 from the nucleus to the mitochondria, and (4) the response of SIRT3 protein to stressors known to induce mitochondrial biogenesis (chronic muscle stimulation and 5-aminoimidazole-4-carboxamide-1-β-d-ribofuranoside administration). SIRT3 protein displayed hierarchical expression based on oxidative potential of muscle tissues (heart >> red >> white). In contrast to studies in some cell lines, metabolic stress (exercise) did not induce the translocation of SIRT3 from the nucleus to mitochondria, as SIRT3 was only present in subsarcolemmal (SS) and intermyofibrillar (IMF) mitochondria, not in the nucleus. Chronic stimulation increased muscle mitochondrial content and SIRT3 protein in SS (+33%) and IMF (+27%) mitochondria (P < .05). In contrast, chronic 5-aminoimidazole-4-carboxamide-1-β-d-ribofuranoside administration, while inducing mitochondrial biogenesis, did not alter SS or IMF mitochondrial SIRT3 protein content. These studies have shown that, in muscle, SIRT3 (a) scales with muscle oxidative capacity and with enzymes regulating fatty acid oxidation, (b) in resting muscle is localized to SS and IMF mitochondria and not nuclei, (c) in contracting muscle is not acutely translocated to mitochondria, and (d) is upregulated with chronic stimulation in an adenosine monophosphate-activated protein kinase-independent manner. SN - 1532-8600 UR - https://www.unboundmedicine.com/medline/citation/22078938/In_mammalian_muscle_SIRT3_is_present_in_mitochondria_and_not_in_the_nucleus L2 - https://linkinghub.elsevier.com/retrieve/pii/S0026-0495(11)00318-0 DB - PRIME DP - Unbound Medicine ER -