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Liquid chromatography-tandem mass spectrometry screening method for the simultaneous detection of stimulants and diuretics in urine.
J Anal Toxicol. 2011 Nov; 35(9):665-74.JA

Abstract

This study established a simultaneous screening method based on solid-phase extraction and liquid chromatography-tandem mass spectrometry (LC-MS-MS) for the detection of 23 stimulants and 23 diuretics in human urine. An electrospray ionization source and multiple reaction monitoring were used for data acquisition. All stimulants and diuretics were separated in less than 12.52 min. The limits of detection were in the range of 25-500 ng/mL for stimulants and 25-125 ng/mL for diuretics. To evaluate the performance of this method, urine samples were collected from 1627 athletes in Taiwan, and 7 positive samples were found. This LC-MS-MS method not only meets the minimum required performance limits set by the World Anti-Doping Agency but also provides a fast way to analyze the authentic urine samples in doping control laboratories.

Authors+Show Affiliations

Graduate Institute of Physical Education, National Taiwan Sport University, Kweishan, Taoyuan County.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22080905

Citation

Hsu, Ku Fu, et al. "Liquid Chromatography-tandem Mass Spectrometry Screening Method for the Simultaneous Detection of Stimulants and Diuretics in Urine." Journal of Analytical Toxicology, vol. 35, no. 9, 2011, pp. 665-74.
Hsu KF, Chien KY, Chang-Chien GP, et al. Liquid chromatography-tandem mass spectrometry screening method for the simultaneous detection of stimulants and diuretics in urine. J Anal Toxicol. 2011;35(9):665-74.
Hsu, K. F., Chien, K. Y., Chang-Chien, G. P., Lin, S. F., Hsu, P. H., & Hsu, M. C. (2011). Liquid chromatography-tandem mass spectrometry screening method for the simultaneous detection of stimulants and diuretics in urine. Journal of Analytical Toxicology, 35(9), 665-74.
Hsu KF, et al. Liquid Chromatography-tandem Mass Spectrometry Screening Method for the Simultaneous Detection of Stimulants and Diuretics in Urine. J Anal Toxicol. 2011;35(9):665-74. PubMed PMID: 22080905.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Liquid chromatography-tandem mass spectrometry screening method for the simultaneous detection of stimulants and diuretics in urine. AU - Hsu,Ku Fu, AU - Chien,Kuei-Yu, AU - Chang-Chien,Guo Ping, AU - Lin,Su Fan, AU - Hsu,Pei Hsuan, AU - Hsu,Mei-Chich, PY - 2011/11/15/entrez PY - 2011/11/15/pubmed PY - 2012/3/8/medline SP - 665 EP - 74 JF - Journal of analytical toxicology JO - J Anal Toxicol VL - 35 IS - 9 N2 - This study established a simultaneous screening method based on solid-phase extraction and liquid chromatography-tandem mass spectrometry (LC-MS-MS) for the detection of 23 stimulants and 23 diuretics in human urine. An electrospray ionization source and multiple reaction monitoring were used for data acquisition. All stimulants and diuretics were separated in less than 12.52 min. The limits of detection were in the range of 25-500 ng/mL for stimulants and 25-125 ng/mL for diuretics. To evaluate the performance of this method, urine samples were collected from 1627 athletes in Taiwan, and 7 positive samples were found. This LC-MS-MS method not only meets the minimum required performance limits set by the World Anti-Doping Agency but also provides a fast way to analyze the authentic urine samples in doping control laboratories. SN - 1945-2403 UR - https://www.unboundmedicine.com/medline/citation/22080905/Liquid_chromatography_tandem_mass_spectrometry_screening_method_for_the_simultaneous_detection_of_stimulants_and_diuretics_in_urine_ L2 - https://academic.oup.com/jat/article-lookup/doi/10.1093/anatox/35.9.665 DB - PRIME DP - Unbound Medicine ER -