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Immunoglobulin G antibodies against deamidated-gliadin-peptides outperform anti-endomysium and tissue transglutaminase antibodies in children <2 years age.
APMIS 2011; 119(12):894-900A

Abstract

To investigate the usefulness of deamidated-gliadin-peptides-antibodies in the diagnosis of celiac disease, serology was tested in 212 children suspected with celiac disease who had undergone a small-intestinal-biopsy. For deamidated-gliadin-peptides-antibodies, two kits were tested. Positive and negative predictive values for IgA deamidated-gliadin-peptides-antibodies using the Bindazyme-kit were 89% and 74%, while the Quanta-Lite-kit had values of 89% and 85%, respectively. For the IgG subtype using the Bindazyme-kit, these values were 85% and 89%, while they were 85% and 91% for the Quanta-Lite-kit. The positive predictive values for endomysium and tissue-transglutaminase antibodies were disappointing (77% and 87%), although the negative predictive values were better (97% and 96%). When the analysis was restricted to the 41 children aged <2 years, no misclassifications occurred with IgG deamidated-gliadin-peptides-antibodies giving 100% accuracy in both kits. The positive predictive value reached 100% for tissue-transglutaminase antibodies and both kits for IgA deamidated-gliadin-peptides-antibodies, while the negative predictive value was 94% in these assays. Positive and negative predictive values for endomysium antibodies were 96% and 93%, respectively. In conclusion, although deamidated-gliadin-peptides-antibodies do not outperform anti-endomysium antibodies in the total study population, the IgG subtype seems to be the best test in children aged <2 years, reaching 100% accuracy.

Authors+Show Affiliations

Department of Pediatric Gastroenterology, University Medical Center Utrecht, Utrecht, The Netherlands. A.Mubarak@umcutrecht.nlNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22085366

Citation

Mubarak, A, et al. "Immunoglobulin G Antibodies Against Deamidated-gliadin-peptides Outperform Anti-endomysium and Tissue Transglutaminase Antibodies in Children <2 Years Age." APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica, vol. 119, no. 12, 2011, pp. 894-900.
Mubarak A, Gmelig-Meyling FH, Wolters VM, et al. Immunoglobulin G antibodies against deamidated-gliadin-peptides outperform anti-endomysium and tissue transglutaminase antibodies in children <2 years age. APMIS. 2011;119(12):894-900.
Mubarak, A., Gmelig-Meyling, F. H., Wolters, V. M., Ten Kate, F. J., & Houwen, R. H. (2011). Immunoglobulin G antibodies against deamidated-gliadin-peptides outperform anti-endomysium and tissue transglutaminase antibodies in children <2 years age. APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica, 119(12), pp. 894-900. doi:10.1111/j.1600-0463.2011.02817.x.
Mubarak A, et al. Immunoglobulin G Antibodies Against Deamidated-gliadin-peptides Outperform Anti-endomysium and Tissue Transglutaminase Antibodies in Children <2 Years Age. APMIS. 2011;119(12):894-900. PubMed PMID: 22085366.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Immunoglobulin G antibodies against deamidated-gliadin-peptides outperform anti-endomysium and tissue transglutaminase antibodies in children <2 years age. AU - Mubarak,A, AU - Gmelig-Meyling,F H J, AU - Wolters,V M, AU - Ten Kate,F J W, AU - Houwen,R H J, Y1 - 2011/09/22/ PY - 2011/11/17/entrez PY - 2011/11/17/pubmed PY - 2011/12/28/medline SP - 894 EP - 900 JF - APMIS : acta pathologica, microbiologica, et immunologica Scandinavica JO - APMIS VL - 119 IS - 12 N2 - To investigate the usefulness of deamidated-gliadin-peptides-antibodies in the diagnosis of celiac disease, serology was tested in 212 children suspected with celiac disease who had undergone a small-intestinal-biopsy. For deamidated-gliadin-peptides-antibodies, two kits were tested. Positive and negative predictive values for IgA deamidated-gliadin-peptides-antibodies using the Bindazyme-kit were 89% and 74%, while the Quanta-Lite-kit had values of 89% and 85%, respectively. For the IgG subtype using the Bindazyme-kit, these values were 85% and 89%, while they were 85% and 91% for the Quanta-Lite-kit. The positive predictive values for endomysium and tissue-transglutaminase antibodies were disappointing (77% and 87%), although the negative predictive values were better (97% and 96%). When the analysis was restricted to the 41 children aged <2 years, no misclassifications occurred with IgG deamidated-gliadin-peptides-antibodies giving 100% accuracy in both kits. The positive predictive value reached 100% for tissue-transglutaminase antibodies and both kits for IgA deamidated-gliadin-peptides-antibodies, while the negative predictive value was 94% in these assays. Positive and negative predictive values for endomysium antibodies were 96% and 93%, respectively. In conclusion, although deamidated-gliadin-peptides-antibodies do not outperform anti-endomysium antibodies in the total study population, the IgG subtype seems to be the best test in children aged <2 years, reaching 100% accuracy. SN - 1600-0463 UR - https://www.unboundmedicine.com/medline/citation/22085366/Immunoglobulin_G_antibodies_against_deamidated_gliadin_peptides_outperform_anti_endomysium_and_tissue_transglutaminase_antibodies_in_children_<2_years_age_ L2 - https://doi.org/10.1111/j.1600-0463.2011.02817.x DB - PRIME DP - Unbound Medicine ER -