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Tricin 7-glucoside protects against experimental cerebral ischemia by reduction of NF-κB and HMGB1 expression.
Eur J Pharm Sci. 2012 Jan 23; 45(1-2):50-7.EJ

Abstract

There have been several studies of nuclear factor-κB (NF-κB) and high-mobility group box1 (HMGB1) associated with the pathophysiology of cerebral ischemia. Tricin 7-glucoside, a major bioactive compound extracted from Sedum sarmentosum Bunge. The objectives of this study were to determine the effects of Tricin 7-glucoside on a cultured neuronal cell line, SH-SY5Y in vitro and experimental ischemic stroke in vivo. For oxygen-glucose deprivation (OGD) and tumor necrosis factor-α (TNF-α) stimulated SH-SY5Y cell line in vitro, SH-SY5Y cells were incubated with Tricin 7-glucoside. For in vivo experiment, rats were subjected to middle cerebral artery occlusion (MACO) for 1h, then followed by reperfusion for 23 h. Treatment of SH-SY5Y cells with Tricin 7-glucoside reduced the OGD-induced apoptosis and cytotoxicity, blocked TNF-α-induced NF-κB and IκB-α phosphorylation, and decreased HMGB1 expression. At doses higher than 50mg/kg, Tricin 7-glucoside produced a significant neuroprotective potential in rats with ischemia and reperfusion (I/R). Tricin 7-glucoside (100mg/kg) demonstrated significant neuroprotective activity even after delayed administration at 2h and 4h after I/R. Tricin 7-glucoside 100mg/kg attenuated histopathological damage, decreased brain edema, inhibited NF-κB activation and reduced HMGB1 expression. These data show that Tricin 7-glucoside protects brain against I/R injury with a favorable therapeutic time-window by alleviating cerebral I/R injury and attenuating blood-brain barrier (BBB) breakdown, and its protective effects may involve HMGB1 and NF-κB signaling pathway.

Authors+Show Affiliations

School of Pharmaceutical Sciences and Institute of Material Medica, Binzhou Medical University, Yantai 264003, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22085682

Citation

Jiang, Wang-Lin, et al. "Tricin 7-glucoside Protects Against Experimental Cerebral Ischemia By Reduction of NF-κB and HMGB1 Expression." European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences, vol. 45, no. 1-2, 2012, pp. 50-7.
Jiang WL, Xu Y, Zhang SP, et al. Tricin 7-glucoside protects against experimental cerebral ischemia by reduction of NF-κB and HMGB1 expression. Eur J Pharm Sci. 2012;45(1-2):50-7.
Jiang, W. L., Xu, Y., Zhang, S. P., Zhu, H. B., & Hou, J. (2012). Tricin 7-glucoside protects against experimental cerebral ischemia by reduction of NF-κB and HMGB1 expression. European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences, 45(1-2), 50-7. https://doi.org/10.1016/j.ejps.2011.10.019
Jiang WL, et al. Tricin 7-glucoside Protects Against Experimental Cerebral Ischemia By Reduction of NF-κB and HMGB1 Expression. Eur J Pharm Sci. 2012 Jan 23;45(1-2):50-7. PubMed PMID: 22085682.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Tricin 7-glucoside protects against experimental cerebral ischemia by reduction of NF-κB and HMGB1 expression. AU - Jiang,Wang-Lin, AU - Xu,Yong, AU - Zhang,Shu-Ping, AU - Zhu,Hai-Bo, AU - Hou,Jian, Y1 - 2011/11/07/ PY - 2011/09/16/received PY - 2011/10/11/revised PY - 2011/10/27/accepted PY - 2011/11/17/entrez PY - 2011/11/17/pubmed PY - 2012/5/5/medline SP - 50 EP - 7 JF - European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences JO - Eur J Pharm Sci VL - 45 IS - 1-2 N2 - There have been several studies of nuclear factor-κB (NF-κB) and high-mobility group box1 (HMGB1) associated with the pathophysiology of cerebral ischemia. Tricin 7-glucoside, a major bioactive compound extracted from Sedum sarmentosum Bunge. The objectives of this study were to determine the effects of Tricin 7-glucoside on a cultured neuronal cell line, SH-SY5Y in vitro and experimental ischemic stroke in vivo. For oxygen-glucose deprivation (OGD) and tumor necrosis factor-α (TNF-α) stimulated SH-SY5Y cell line in vitro, SH-SY5Y cells were incubated with Tricin 7-glucoside. For in vivo experiment, rats were subjected to middle cerebral artery occlusion (MACO) for 1h, then followed by reperfusion for 23 h. Treatment of SH-SY5Y cells with Tricin 7-glucoside reduced the OGD-induced apoptosis and cytotoxicity, blocked TNF-α-induced NF-κB and IκB-α phosphorylation, and decreased HMGB1 expression. At doses higher than 50mg/kg, Tricin 7-glucoside produced a significant neuroprotective potential in rats with ischemia and reperfusion (I/R). Tricin 7-glucoside (100mg/kg) demonstrated significant neuroprotective activity even after delayed administration at 2h and 4h after I/R. Tricin 7-glucoside 100mg/kg attenuated histopathological damage, decreased brain edema, inhibited NF-κB activation and reduced HMGB1 expression. These data show that Tricin 7-glucoside protects brain against I/R injury with a favorable therapeutic time-window by alleviating cerebral I/R injury and attenuating blood-brain barrier (BBB) breakdown, and its protective effects may involve HMGB1 and NF-κB signaling pathway. SN - 1879-0720 UR - https://www.unboundmedicine.com/medline/citation/22085682/Tricin_7_glucoside_protects_against_experimental_cerebral_ischemia_by_reduction_of_NF_κB_and_HMGB1_expression_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0928-0987(11)00379-4 DB - PRIME DP - Unbound Medicine ER -