Tags

Type your tag names separated by a space and hit enter

Prevalence and sequence variation of panton-valentine leukocidin in methicillin-resistant and methicillin-susceptible staphylococcus aureus strains in the United States.
J Clin Microbiol. 2012 Jan; 50(1):86-90.JC

Abstract

Panton-Valentine leukocidin (PVL), encoded by the lukSF-PV genes, is a putative virulence factor and marker for community-associated methicillin-resistant Staphylococcus aureus. Here we report the prevalence of PVL among a representative sample of 1,055 S. aureus infection isolates from the United States and describe the sequence variation of the lukSF-PV genes. We performed multilocus sequence typing (MLST) on all isolates and sequenced fragments of the lukSF-PV genes from a sample of 86 isolates. We assigned isolates to a PVL R or H sequence type based on a polymorphism that results in an amino acid change from arginine (R) to histidine (H). Overall, we found that 36% of S. aureus isolates were positive for lukSF-PV. Among the 86 we typed, we identified 72 R variants and 14 H variants. Among the 47 methicillin-resistance S. aureus (MRSA) isolates, 43 harbored the R variant, and among the 39 methicillin-susceptible S. aureus (MSSA) isolates, 29 harbored the R variant. Almost all (97%) of the R variants were found in MLST clonal complex 8 (CC8), while the H variant was broadly distributed among 6 CCs. Within CC8, all 38 MRSA (USA300) and all 28 MSSA isolates harbored the R variant. Of the 20 isolates from blood and the lower respiratory tract, 19 (95%) harbored the R variant. While the R variant had been linked primarily to USA300 MRSA, we found that all CC8 MSSA isolates also contained the R variant, suggesting that some strains of USA300 may have lost methicillin resistance as an adaptation in the community.

Authors+Show Affiliations

Department of Computational Biology, GlaxoSmithKline, Collegeville, Pennsylvania, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22090402

Citation

Brown, Megan L., et al. "Prevalence and Sequence Variation of Panton-valentine Leukocidin in Methicillin-resistant and Methicillin-susceptible Staphylococcus Aureus Strains in the United States." Journal of Clinical Microbiology, vol. 50, no. 1, 2012, pp. 86-90.
Brown ML, O'Hara FP, Close NM, et al. Prevalence and sequence variation of panton-valentine leukocidin in methicillin-resistant and methicillin-susceptible staphylococcus aureus strains in the United States. J Clin Microbiol. 2012;50(1):86-90.
Brown, M. L., O'Hara, F. P., Close, N. M., Mera, R. M., Miller, L. A., Suaya, J. A., & Amrine-Madsen, H. (2012). Prevalence and sequence variation of panton-valentine leukocidin in methicillin-resistant and methicillin-susceptible staphylococcus aureus strains in the United States. Journal of Clinical Microbiology, 50(1), 86-90. https://doi.org/10.1128/JCM.05564-11
Brown ML, et al. Prevalence and Sequence Variation of Panton-valentine Leukocidin in Methicillin-resistant and Methicillin-susceptible Staphylococcus Aureus Strains in the United States. J Clin Microbiol. 2012;50(1):86-90. PubMed PMID: 22090402.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Prevalence and sequence variation of panton-valentine leukocidin in methicillin-resistant and methicillin-susceptible staphylococcus aureus strains in the United States. AU - Brown,Megan L, AU - O'Hara,F Patrick, AU - Close,Nicole M, AU - Mera,Robertino M, AU - Miller,Linda A, AU - Suaya,Jose A, AU - Amrine-Madsen,Heather, Y1 - 2011/11/16/ PY - 2011/11/18/entrez PY - 2011/11/18/pubmed PY - 2012/4/12/medline SP - 86 EP - 90 JF - Journal of clinical microbiology JO - J Clin Microbiol VL - 50 IS - 1 N2 - Panton-Valentine leukocidin (PVL), encoded by the lukSF-PV genes, is a putative virulence factor and marker for community-associated methicillin-resistant Staphylococcus aureus. Here we report the prevalence of PVL among a representative sample of 1,055 S. aureus infection isolates from the United States and describe the sequence variation of the lukSF-PV genes. We performed multilocus sequence typing (MLST) on all isolates and sequenced fragments of the lukSF-PV genes from a sample of 86 isolates. We assigned isolates to a PVL R or H sequence type based on a polymorphism that results in an amino acid change from arginine (R) to histidine (H). Overall, we found that 36% of S. aureus isolates were positive for lukSF-PV. Among the 86 we typed, we identified 72 R variants and 14 H variants. Among the 47 methicillin-resistance S. aureus (MRSA) isolates, 43 harbored the R variant, and among the 39 methicillin-susceptible S. aureus (MSSA) isolates, 29 harbored the R variant. Almost all (97%) of the R variants were found in MLST clonal complex 8 (CC8), while the H variant was broadly distributed among 6 CCs. Within CC8, all 38 MRSA (USA300) and all 28 MSSA isolates harbored the R variant. Of the 20 isolates from blood and the lower respiratory tract, 19 (95%) harbored the R variant. While the R variant had been linked primarily to USA300 MRSA, we found that all CC8 MSSA isolates also contained the R variant, suggesting that some strains of USA300 may have lost methicillin resistance as an adaptation in the community. SN - 1098-660X UR - https://www.unboundmedicine.com/medline/citation/22090402/Prevalence_and_sequence_variation_of_panton_valentine_leukocidin_in_methicillin_resistant_and_methicillin_susceptible_staphylococcus_aureus_strains_in_the_United_States_ L2 - http://jcm.asm.org/cgi/pmidlookup?view=long&pmid=22090402 DB - PRIME DP - Unbound Medicine ER -