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Rapid characterization and identification of steroidal alkaloids in Sarcococca coriacea using liquid chromatography coupled with electrospray ionization quadropole time-of-flight mass spectrometry.
Steroids. 2012 Jan; 77(1-2):138-48.S

Abstract

Rapid characterization of 23 pregnane-type steroidal alkaloids was studied using a positive ion electrospray ionization quadropole time-of-flight mass spectrometry (ESI-QqTOF-MS/MS) hybrid instrument. ESI-QqTOF-MS (positive ion mode) showed the presence of the protonated molecules [M+H](+) which through low-energy collision-induced dissociation tandem mass spectrometric (CID-MS/MS) analysis showed the characteristic loss of dimethylamine moiety [M+H-45](+) followed by the sequential lossess of attached substituents. Steroidal alkaloids having tigloyl or senecioyl group at C-3 produced diagnostic fragment ions at m/z 100 and 83. Our study also demonstrates the influence of unsaturation, and number and nature of substitutents on product ion abundance and fragment ions. Moreover, the generalization of the fragmentation pattern was linked with the structural features in steroidal skeleton. This strategy was successfully applied in LC-ESI-QqTOF-MS/MS analysis of Sarcococca coriacea extract to investigate and characterize pregnane-type steroidal alkaloids in complex mixture.

Authors+Show Affiliations

HEJ Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi, Pakistan. musharraf1977@yahoo.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22093483

Citation

Musharraf, Syed Ghulam, et al. "Rapid Characterization and Identification of Steroidal Alkaloids in Sarcococca Coriacea Using Liquid Chromatography Coupled With Electrospray Ionization Quadropole Time-of-flight Mass Spectrometry." Steroids, vol. 77, no. 1-2, 2012, pp. 138-48.
Musharraf SG, Goher M, Ali A, et al. Rapid characterization and identification of steroidal alkaloids in Sarcococca coriacea using liquid chromatography coupled with electrospray ionization quadropole time-of-flight mass spectrometry. Steroids. 2012;77(1-2):138-48.
Musharraf, S. G., Goher, M., Ali, A., Adhikari, A., Choudhary, M. I., & Atta-ur-Rahman, . (2012). Rapid characterization and identification of steroidal alkaloids in Sarcococca coriacea using liquid chromatography coupled with electrospray ionization quadropole time-of-flight mass spectrometry. Steroids, 77(1-2), 138-48. https://doi.org/10.1016/j.steroids.2011.11.001
Musharraf SG, et al. Rapid Characterization and Identification of Steroidal Alkaloids in Sarcococca Coriacea Using Liquid Chromatography Coupled With Electrospray Ionization Quadropole Time-of-flight Mass Spectrometry. Steroids. 2012;77(1-2):138-48. PubMed PMID: 22093483.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rapid characterization and identification of steroidal alkaloids in Sarcococca coriacea using liquid chromatography coupled with electrospray ionization quadropole time-of-flight mass spectrometry. AU - Musharraf,Syed Ghulam, AU - Goher,Madiha, AU - Ali,Arslan, AU - Adhikari,Achyut, AU - Choudhary,M Iqbal, AU - Atta-ur-Rahman,, Y1 - 2011/11/09/ PY - 2011/08/30/received PY - 2011/10/30/revised PY - 2011/11/01/accepted PY - 2011/11/19/entrez PY - 2011/11/19/pubmed PY - 2012/6/23/medline SP - 138 EP - 48 JF - Steroids JO - Steroids VL - 77 IS - 1-2 N2 - Rapid characterization of 23 pregnane-type steroidal alkaloids was studied using a positive ion electrospray ionization quadropole time-of-flight mass spectrometry (ESI-QqTOF-MS/MS) hybrid instrument. ESI-QqTOF-MS (positive ion mode) showed the presence of the protonated molecules [M+H](+) which through low-energy collision-induced dissociation tandem mass spectrometric (CID-MS/MS) analysis showed the characteristic loss of dimethylamine moiety [M+H-45](+) followed by the sequential lossess of attached substituents. Steroidal alkaloids having tigloyl or senecioyl group at C-3 produced diagnostic fragment ions at m/z 100 and 83. Our study also demonstrates the influence of unsaturation, and number and nature of substitutents on product ion abundance and fragment ions. Moreover, the generalization of the fragmentation pattern was linked with the structural features in steroidal skeleton. This strategy was successfully applied in LC-ESI-QqTOF-MS/MS analysis of Sarcococca coriacea extract to investigate and characterize pregnane-type steroidal alkaloids in complex mixture. SN - 1878-5867 UR - https://www.unboundmedicine.com/medline/citation/22093483/Rapid_characterization_and_identification_of_steroidal_alkaloids_in_Sarcococca_coriacea_using_liquid_chromatography_coupled_with_electrospray_ionization_quadropole_time_of_flight_mass_spectrometry_ DB - PRIME DP - Unbound Medicine ER -