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Anaerobic oxidation of methane at different temperature regimes in Guaymas Basin hydrothermal sediments.
ISME J. 2012 May; 6(5):1018-31.IJ

Abstract

Anaerobic oxidation of methane (AOM) was investigated in hydrothermal sediments of Guaymas Basin based on δ(13)C signatures of CH(4), dissolved inorganic carbon and porewater concentration profiles of CH(4) and sulfate. Cool, warm and hot in-situ temperature regimes (15-20 °C, 30-35 °C and 70-95 °C) were selected from hydrothermal locations in Guaymas Basin to compare AOM geochemistry and 16S ribosomal RNA (rRNA), mcrA and dsrAB genes of the microbial communities. 16S rRNA gene clone libraries from the cool and hot AOM cores yielded similar archaeal types such as Miscellaneous Crenarchaeotal Group, Thermoproteales and anaerobic methane-oxidizing archaea (ANME)-1; some of the ANME-1 archaea formed a separate 16S rRNA lineage that at present seems to be limited to Guaymas Basin. Congruent results were obtained by mcrA gene analysis. The warm AOM core, chemically distinct by lower porewater sulfide concentrations, hosted a different archaeal community dominated by the two deep subsurface archaeal lineages Marine Benthic Group D and Marine Benthic Group B, and by members of the Methanosarcinales including ANME-2 archaea. This distinct composition of the methane-cycling archaeal community in the warm AOM core was confirmed by mcrA gene analysis. Functional genes of sulfate-reducing bacteria and archaea, dsrAB, showed more overlap between all cores, regardless of the core temperature. 16S rRNA gene clone libraries with Euryarchaeota-specific primers detected members of the Archaeoglobus clade in the cool and hot cores. A V6-tag high-throughput sequencing survey generally supported the clone library results while providing high-resolution detail on archaeal and bacterial community structure. These results indicate that AOM and the responsible archaeal communities persist over a wide temperature range.

Authors+Show Affiliations

School of Marine Science and Policy, University of Delaware, Lewes, DE, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

22094346

Citation

Biddle, Jennifer F., et al. "Anaerobic Oxidation of Methane at Different Temperature Regimes in Guaymas Basin Hydrothermal Sediments." The ISME Journal, vol. 6, no. 5, 2012, pp. 1018-31.
Biddle JF, Cardman Z, Mendlovitz H, et al. Anaerobic oxidation of methane at different temperature regimes in Guaymas Basin hydrothermal sediments. ISME J. 2012;6(5):1018-31.
Biddle, J. F., Cardman, Z., Mendlovitz, H., Albert, D. B., Lloyd, K. G., Boetius, A., & Teske, A. (2012). Anaerobic oxidation of methane at different temperature regimes in Guaymas Basin hydrothermal sediments. The ISME Journal, 6(5), 1018-31. https://doi.org/10.1038/ismej.2011.164
Biddle JF, et al. Anaerobic Oxidation of Methane at Different Temperature Regimes in Guaymas Basin Hydrothermal Sediments. ISME J. 2012;6(5):1018-31. PubMed PMID: 22094346.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Anaerobic oxidation of methane at different temperature regimes in Guaymas Basin hydrothermal sediments. AU - Biddle,Jennifer F, AU - Cardman,Zena, AU - Mendlovitz,Howard, AU - Albert,Daniel B, AU - Lloyd,Karen G, AU - Boetius,Antje, AU - Teske,Andreas, Y1 - 2011/11/17/ PY - 2011/11/19/entrez PY - 2011/11/19/pubmed PY - 2012/11/14/medline SP - 1018 EP - 31 JF - The ISME journal JO - ISME J VL - 6 IS - 5 N2 - Anaerobic oxidation of methane (AOM) was investigated in hydrothermal sediments of Guaymas Basin based on δ(13)C signatures of CH(4), dissolved inorganic carbon and porewater concentration profiles of CH(4) and sulfate. Cool, warm and hot in-situ temperature regimes (15-20 °C, 30-35 °C and 70-95 °C) were selected from hydrothermal locations in Guaymas Basin to compare AOM geochemistry and 16S ribosomal RNA (rRNA), mcrA and dsrAB genes of the microbial communities. 16S rRNA gene clone libraries from the cool and hot AOM cores yielded similar archaeal types such as Miscellaneous Crenarchaeotal Group, Thermoproteales and anaerobic methane-oxidizing archaea (ANME)-1; some of the ANME-1 archaea formed a separate 16S rRNA lineage that at present seems to be limited to Guaymas Basin. Congruent results were obtained by mcrA gene analysis. The warm AOM core, chemically distinct by lower porewater sulfide concentrations, hosted a different archaeal community dominated by the two deep subsurface archaeal lineages Marine Benthic Group D and Marine Benthic Group B, and by members of the Methanosarcinales including ANME-2 archaea. This distinct composition of the methane-cycling archaeal community in the warm AOM core was confirmed by mcrA gene analysis. Functional genes of sulfate-reducing bacteria and archaea, dsrAB, showed more overlap between all cores, regardless of the core temperature. 16S rRNA gene clone libraries with Euryarchaeota-specific primers detected members of the Archaeoglobus clade in the cool and hot cores. A V6-tag high-throughput sequencing survey generally supported the clone library results while providing high-resolution detail on archaeal and bacterial community structure. These results indicate that AOM and the responsible archaeal communities persist over a wide temperature range. SN - 1751-7370 UR - https://www.unboundmedicine.com/medline/citation/22094346/Anaerobic_oxidation_of_methane_at_different_temperature_regimes_in_Guaymas_Basin_hydrothermal_sediments_ L2 - http://dx.doi.org/10.1038/ismej.2011.164 DB - PRIME DP - Unbound Medicine ER -