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Interaction of the main components from the traditional Chinese drug pair Chaihu-Shaoyao based on rat intestinal absorption.
Molecules 2011; 16(11):9600-10M

Abstract

The Chaihu-Shaoyao drug pair (Bupleuri Radix and Paeoniae Radix Alba) which is a traditional Chinese drug pair, has been widely used for anti-inflammatory purposes. Saikosaponin a (SSA), saikosaponin d (SSD) and paeoniflorin are identified as the main components in the pair. The present study focused on the interaction of the main components based on investigating their intestinal absorption using a four-site perfused rat intestinal model in order to clarify the mechanism of the compatibility of Chaihu-Shaoyao. The concentrations of SSA, SSD and paeoniflorin in the intestinal perfusate were determined by LC/MS or UPLC (Ultra Performance Liquid Chromatography) methods, followed by P*(eff) (effective permeability) and 10% ABS (the percent absorption of 10 cm of intestine) calculations. The results showed that all of the three main components displayed very low permeabilities (P*(eff) < 0.4), which implied their poor absorption in the rat intestine. The absorption levels of SSA and SSD were similar in intestine and higher in ileum than those in other intestinal regions in the decreasing order: colon, jejunum and duodenum. However, there is no significant difference in the absorption of paeoniflorin in the four segments (P < 0.05). The P*(eff) values of paeoniflorin exhibited an almost 2.11-fold or 1.90-fold increase in ileum when it was co-administrated with SSA and SSD, as well as 2.42-, 2.18-fold increase in colon, respectively, whereas the absorptions of SSA and SSD were not influenced by paeoniflorin. In conclusion, SSA and SSD could promote the absorption of paeoniflorin. To some extent this might explain the nature of the compatibility mechanisms of composite formulae in TCMs.

Authors+Show Affiliations

Key Laboratory of New Drug Delivery System of Chinese Material Medica, Jiangsu Province, Academy of Chinese Medicine, 100 Shizi Road, Nanjing 210028, China. ychen202@yahoo.com.cnNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22095024

Citation

Chen, Yan, et al. "Interaction of the Main Components From the Traditional Chinese Drug Pair Chaihu-Shaoyao Based On Rat Intestinal Absorption." Molecules (Basel, Switzerland), vol. 16, no. 11, 2011, pp. 9600-10.
Chen Y, Wang J, Yuan L, et al. Interaction of the main components from the traditional Chinese drug pair Chaihu-Shaoyao based on rat intestinal absorption. Molecules. 2011;16(11):9600-10.
Chen, Y., Wang, J., Yuan, L., Zhou, L., Jia, X., & Tan, X. (2011). Interaction of the main components from the traditional Chinese drug pair Chaihu-Shaoyao based on rat intestinal absorption. Molecules (Basel, Switzerland), 16(11), pp. 9600-10. doi:10.3390/molecules16119600.
Chen Y, et al. Interaction of the Main Components From the Traditional Chinese Drug Pair Chaihu-Shaoyao Based On Rat Intestinal Absorption. Molecules. 2011 Nov 17;16(11):9600-10. PubMed PMID: 22095024.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Interaction of the main components from the traditional Chinese drug pair Chaihu-Shaoyao based on rat intestinal absorption. AU - Chen,Yan, AU - Wang,Jinyan, AU - Yuan,Ling, AU - Zhou,Lei, AU - Jia,Xiaobin, AU - Tan,Xiaobin, Y1 - 2011/11/17/ PY - 2011/10/18/received PY - 2011/11/08/revised PY - 2011/11/10/accepted PY - 2011/11/19/entrez PY - 2011/11/19/pubmed PY - 2012/4/14/medline SP - 9600 EP - 10 JF - Molecules (Basel, Switzerland) JO - Molecules VL - 16 IS - 11 N2 - The Chaihu-Shaoyao drug pair (Bupleuri Radix and Paeoniae Radix Alba) which is a traditional Chinese drug pair, has been widely used for anti-inflammatory purposes. Saikosaponin a (SSA), saikosaponin d (SSD) and paeoniflorin are identified as the main components in the pair. The present study focused on the interaction of the main components based on investigating their intestinal absorption using a four-site perfused rat intestinal model in order to clarify the mechanism of the compatibility of Chaihu-Shaoyao. The concentrations of SSA, SSD and paeoniflorin in the intestinal perfusate were determined by LC/MS or UPLC (Ultra Performance Liquid Chromatography) methods, followed by P*(eff) (effective permeability) and 10% ABS (the percent absorption of 10 cm of intestine) calculations. The results showed that all of the three main components displayed very low permeabilities (P*(eff) < 0.4), which implied their poor absorption in the rat intestine. The absorption levels of SSA and SSD were similar in intestine and higher in ileum than those in other intestinal regions in the decreasing order: colon, jejunum and duodenum. However, there is no significant difference in the absorption of paeoniflorin in the four segments (P < 0.05). The P*(eff) values of paeoniflorin exhibited an almost 2.11-fold or 1.90-fold increase in ileum when it was co-administrated with SSA and SSD, as well as 2.42-, 2.18-fold increase in colon, respectively, whereas the absorptions of SSA and SSD were not influenced by paeoniflorin. In conclusion, SSA and SSD could promote the absorption of paeoniflorin. To some extent this might explain the nature of the compatibility mechanisms of composite formulae in TCMs. SN - 1420-3049 UR - https://www.unboundmedicine.com/medline/citation/22095024/Interaction_of_the_main_components_from_the_traditional_Chinese_drug_pair_Chaihu_Shaoyao_based_on_rat_intestinal_absorption_ L2 - http://www.mdpi.com/resolver?pii=molecules16119600 DB - PRIME DP - Unbound Medicine ER -