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[Effects of Mycobacterium phlei F.U.36 on regulatory T cells and TLR4 expression in asthmatic mice].
Zhongguo Dang Dai Er Ke Za Zhi. 2011 Nov; 13(11):917-20.ZD

Abstract

OBJECTIVE

To study the effects of early intervention on CD4+CD25+ regulatory T cells and TLR4 expression with Mycobacterium phlei F.U.36 in asthmatic mice.

METHODS

Thirty female BALB/c mice were randomly divided into three groups: control, asthma model and Mycobacterium phlei F.U.36 treated asthma groups. Asthma was induced by sensitization and challenges with ovalbumin (OVA) in the later two groups. Mycobacterium phlei F.U.36 was intraperitoneally injected 2 weeks before the first sensitization (0.57 μg/time, once every other day for three times) in the intervention group. After 24 hrs of the last challenge, the mice were sacrificed and the left lung tissues were obtained for the observation of lung pathological changes. Splenic mononuclear cells were isolated. The percentage of CD4+CD25+ regulatory T cells in CD4+ T cells and the mean fluorescence intensity of TLR4 on CD4+ CD25+ T cells were detected by flow cytometry.

RESULTS

The percentage of CD4+CD25+ regulatory T cells in the asthma model group was significantly lower than that in the control group (P<0.01), but the mean fluorescence intensity of TLR4 on CD4+CD25+ regulatory T cells was not significantly different from the control group. The percentage of CD4+CD25+ regulatory T cells and the mean fluorescence intensity of TLR4 on CD4+CD25+ regulatory T cells increased significantly in asthmatic mice receiving Mycobacterium phlei F.U.36 treatment compared with the asthma group (P<0.01).

CONCLUSIONS

Early intervention with Mycobacterium phlei F.U.36 can increase TLR4 expression on CD4+CD25+ cells and the number of CD4+CD25+ regulatory T cells, and thus provides therapeutic effects in asthmatic mice.

Authors+Show Affiliations

Department of Pediatrics, Sichuan Provincial People's Hospital, Chengdu, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

22099205

Citation

Pang, Ying, et al. "[Effects of Mycobacterium Phlei F.U.36 On Regulatory T Cells and TLR4 Expression in Asthmatic Mice]." Zhongguo Dang Dai Er Ke Za Zhi = Chinese Journal of Contemporary Pediatrics, vol. 13, no. 11, 2011, pp. 917-20.
Pang Y, Li M, Zhang JB, et al. [Effects of Mycobacterium phlei F.U.36 on regulatory T cells and TLR4 expression in asthmatic mice]. Zhongguo Dang Dai Er Ke Za Zhi. 2011;13(11):917-20.
Pang, Y., Li, M., Zhang, J. B., & Yao, B. (2011). [Effects of Mycobacterium phlei F.U.36 on regulatory T cells and TLR4 expression in asthmatic mice]. Zhongguo Dang Dai Er Ke Za Zhi = Chinese Journal of Contemporary Pediatrics, 13(11), 917-20.
Pang Y, et al. [Effects of Mycobacterium Phlei F.U.36 On Regulatory T Cells and TLR4 Expression in Asthmatic Mice]. Zhongguo Dang Dai Er Ke Za Zhi. 2011;13(11):917-20. PubMed PMID: 22099205.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effects of Mycobacterium phlei F.U.36 on regulatory T cells and TLR4 expression in asthmatic mice]. AU - Pang,Ying, AU - Li,Min, AU - Zhang,Jian-Bo, AU - Yao,Bin, PY - 2011/11/22/entrez PY - 2011/11/22/pubmed PY - 2012/2/15/medline SP - 917 EP - 20 JF - Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics JO - Zhongguo Dang Dai Er Ke Za Zhi VL - 13 IS - 11 N2 - OBJECTIVE: To study the effects of early intervention on CD4+CD25+ regulatory T cells and TLR4 expression with Mycobacterium phlei F.U.36 in asthmatic mice. METHODS: Thirty female BALB/c mice were randomly divided into three groups: control, asthma model and Mycobacterium phlei F.U.36 treated asthma groups. Asthma was induced by sensitization and challenges with ovalbumin (OVA) in the later two groups. Mycobacterium phlei F.U.36 was intraperitoneally injected 2 weeks before the first sensitization (0.57 μg/time, once every other day for three times) in the intervention group. After 24 hrs of the last challenge, the mice were sacrificed and the left lung tissues were obtained for the observation of lung pathological changes. Splenic mononuclear cells were isolated. The percentage of CD4+CD25+ regulatory T cells in CD4+ T cells and the mean fluorescence intensity of TLR4 on CD4+ CD25+ T cells were detected by flow cytometry. RESULTS: The percentage of CD4+CD25+ regulatory T cells in the asthma model group was significantly lower than that in the control group (P<0.01), but the mean fluorescence intensity of TLR4 on CD4+CD25+ regulatory T cells was not significantly different from the control group. The percentage of CD4+CD25+ regulatory T cells and the mean fluorescence intensity of TLR4 on CD4+CD25+ regulatory T cells increased significantly in asthmatic mice receiving Mycobacterium phlei F.U.36 treatment compared with the asthma group (P<0.01). CONCLUSIONS: Early intervention with Mycobacterium phlei F.U.36 can increase TLR4 expression on CD4+CD25+ cells and the number of CD4+CD25+ regulatory T cells, and thus provides therapeutic effects in asthmatic mice. SN - 1008-8830 UR - https://www.unboundmedicine.com/medline/citation/22099205/[Effects_of_Mycobacterium_phlei_F_U_36_on_regulatory_T_cells_and_TLR4_expression_in_asthmatic_mice]_ L2 - http://www.zgddek.com/EN/abstract/abstract12702.shtml DB - PRIME DP - Unbound Medicine ER -