Tags

Type your tag names separated by a space and hit enter

Application of chitosan/Fe₃O₄ microsphere-graphene composite modified carbon ionic liquid electrode for the electrochemical detection of the PCR product of soybean Lectin gene sequence.
Talanta. 2011 Dec 15; 87:106-12.T

Abstract

In this paper a Fe(3)O(4) microsphere, graphene (GR) and chitosan (CTS) nanocomposite material modified carbon ionic liquid electrode (CILE) was used as the platform for the construction of a new electrochemical DNA biosensor. The single-stranded DNA (ssDNA) probe was immobilized directly on the surface of the CTS/Fe(3)O(4)-GR/CILE, which could hybridize with the target ssDNA sequence at the selected conditions. By using methylene blue (MB) as the electrochemical indicator the hybridization reaction was investigated with the reduction peak current measured. By combining the specific properties such as the biocompatibility and big surface area of Fe(3)O(4) microspheres, the excellent electron transfer ability of GR, the good film-forming ability of CTS and the high conductivity of CILE, the synergistic effects of nanocomposite increased the amounts of ssDNA adsorbed on the electrode surface and then resulted in the greatly increase of the electrochemical responses. Under the optimal conditions differential pulse voltammetric responses of MB were proportional to the specific ssDNA sequences concentration in the range from 1.0×10(-12) to 1.0×10(-6)mol/L with the detection limit as 3.59×10(-13)mol/L (3σ). This DNA biosensor showed good stability and discrimination ability to one-base and three-base mismatched ssDNA sequences. The polymerase chain reaction (PCR) product of soybean Lectin gene sequence was detected by the proposed method with satisfactory result, suggesting that the CTS/Fe(3)O(4)-GR/CILE was a suitable sensing platform for the sensitive detection of specific gene sequence.

Authors+Show Affiliations

Key Lab of Eco-chemical Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China. sunwei@qust.edu.cnNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22099656

Citation

Sun, Wei, et al. "Application of chitosan/Fe₃O₄ Microsphere-graphene Composite Modified Carbon Ionic Liquid Electrode for the Electrochemical Detection of the PCR Product of Soybean Lectin Gene Sequence." Talanta, vol. 87, 2011, pp. 106-12.
Sun W, Qi X, Chen Y, et al. Application of chitosan/Fe₃O₄ microsphere-graphene composite modified carbon ionic liquid electrode for the electrochemical detection of the PCR product of soybean Lectin gene sequence. Talanta. 2011;87:106-12.
Sun, W., Qi, X., Chen, Y., Liu, S., & Gao, H. (2011). Application of chitosan/Fe₃O₄ microsphere-graphene composite modified carbon ionic liquid electrode for the electrochemical detection of the PCR product of soybean Lectin gene sequence. Talanta, 87, 106-12. https://doi.org/10.1016/j.talanta.2011.09.047
Sun W, et al. Application of chitosan/Fe₃O₄ Microsphere-graphene Composite Modified Carbon Ionic Liquid Electrode for the Electrochemical Detection of the PCR Product of Soybean Lectin Gene Sequence. Talanta. 2011 Dec 15;87:106-12. PubMed PMID: 22099656.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Application of chitosan/Fe₃O₄ microsphere-graphene composite modified carbon ionic liquid electrode for the electrochemical detection of the PCR product of soybean Lectin gene sequence. AU - Sun,Wei, AU - Qi,Xiaowei, AU - Chen,Ying, AU - Liu,Shengyun, AU - Gao,Hongwei, Y1 - 2011/10/01/ PY - 2011/06/09/received PY - 2011/09/12/revised PY - 2011/09/15/accepted PY - 2011/11/22/entrez PY - 2011/11/22/pubmed PY - 2012/3/20/medline SP - 106 EP - 12 JF - Talanta JO - Talanta VL - 87 N2 - In this paper a Fe(3)O(4) microsphere, graphene (GR) and chitosan (CTS) nanocomposite material modified carbon ionic liquid electrode (CILE) was used as the platform for the construction of a new electrochemical DNA biosensor. The single-stranded DNA (ssDNA) probe was immobilized directly on the surface of the CTS/Fe(3)O(4)-GR/CILE, which could hybridize with the target ssDNA sequence at the selected conditions. By using methylene blue (MB) as the electrochemical indicator the hybridization reaction was investigated with the reduction peak current measured. By combining the specific properties such as the biocompatibility and big surface area of Fe(3)O(4) microspheres, the excellent electron transfer ability of GR, the good film-forming ability of CTS and the high conductivity of CILE, the synergistic effects of nanocomposite increased the amounts of ssDNA adsorbed on the electrode surface and then resulted in the greatly increase of the electrochemical responses. Under the optimal conditions differential pulse voltammetric responses of MB were proportional to the specific ssDNA sequences concentration in the range from 1.0×10(-12) to 1.0×10(-6)mol/L with the detection limit as 3.59×10(-13)mol/L (3σ). This DNA biosensor showed good stability and discrimination ability to one-base and three-base mismatched ssDNA sequences. The polymerase chain reaction (PCR) product of soybean Lectin gene sequence was detected by the proposed method with satisfactory result, suggesting that the CTS/Fe(3)O(4)-GR/CILE was a suitable sensing platform for the sensitive detection of specific gene sequence. SN - 1873-3573 UR - https://www.unboundmedicine.com/medline/citation/22099656/Application_of_chitosan/Fe₃O₄_microsphere_graphene_composite_modified_carbon_ionic_liquid_electrode_for_the_electrochemical_detection_of_the_PCR_product_of_soybean_Lectin_gene_sequence_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0039-9140(11)00855-1 DB - PRIME DP - Unbound Medicine ER -