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Cryptic introgression of Dasypyrum villosum parental DNA in wheat lines derived from intergeneric hybridization.
Cytogenet Genome Res. 2012; 136(1):75-81.CG

Abstract

Cytogenetic and DNA molecular analyses have been carried out in 3 wheat introgression lines (ILs; CS×V58, CS×V59, and CS×V60) derived from Triticum aestivum cv. 'Chinese Spring' (CS) × Dasypyrum villosum(Dv) intergeneric hybridization. All lines, which showed several phenotypic differences compared to CS, had the same chromosome number (2n = 42) and structure as CS, and neither chromosomes nor chromatin from Dv were apparently added to their complement. However, Feulgen/DNA cytophotometry showed that there was more nuclear DNA in the lines than in the parental wheat (by 1.85%, 2.76%, and 1.26% in CS×V58, CS×V59, and CS×V60, respectively). Molecular investigation indicated the presence of Dv DNA in the ILs. AFLP analysis of genomic DNA from the ILs, CS, and Dv detected a total of 120 polymorphic bands, of which 7 (5.8%) were present in some or all the ILs and Dv but were absent in CS. PCR amplification, sequence analysis of amplicons, and Southern blot hybridization confirmed the presence of Dv-specific sequences in each of the ILs. These results indicate cryptic introgression of Dv DNA sequences into the genome of the ILs. Some implications of this finding are discussed.

Authors+Show Affiliations

Dipartimento di Biologia Cellulare e Ambientale, Sezione di Biologia Cellulare e Molecolare, Università di Perugia, Italia. cionini@unipg.itNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22104134

Citation

Caceres, M E., et al. "Cryptic Introgression of Dasypyrum Villosum Parental DNA in Wheat Lines Derived From Intergeneric Hybridization." Cytogenetic and Genome Research, vol. 136, no. 1, 2012, pp. 75-81.
Caceres ME, Pupilli F, Ceccarelli M, et al. Cryptic introgression of Dasypyrum villosum parental DNA in wheat lines derived from intergeneric hybridization. Cytogenet Genome Res. 2012;136(1):75-81.
Caceres, M. E., Pupilli, F., Ceccarelli, M., Vaccino, P., Sarri, V., De Pace, C., & Cionini, P. G. (2012). Cryptic introgression of Dasypyrum villosum parental DNA in wheat lines derived from intergeneric hybridization. Cytogenetic and Genome Research, 136(1), 75-81. https://doi.org/10.1159/000334275
Caceres ME, et al. Cryptic Introgression of Dasypyrum Villosum Parental DNA in Wheat Lines Derived From Intergeneric Hybridization. Cytogenet Genome Res. 2012;136(1):75-81. PubMed PMID: 22104134.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cryptic introgression of Dasypyrum villosum parental DNA in wheat lines derived from intergeneric hybridization. AU - Caceres,M E, AU - Pupilli,F, AU - Ceccarelli,M, AU - Vaccino,P, AU - Sarri,V, AU - De Pace,C, AU - Cionini,P G, Y1 - 2011/11/18/ PY - 2011/09/07/accepted PY - 2011/11/23/entrez PY - 2011/11/23/pubmed PY - 2012/6/6/medline SP - 75 EP - 81 JF - Cytogenetic and genome research JO - Cytogenet Genome Res VL - 136 IS - 1 N2 - Cytogenetic and DNA molecular analyses have been carried out in 3 wheat introgression lines (ILs; CS×V58, CS×V59, and CS×V60) derived from Triticum aestivum cv. 'Chinese Spring' (CS) × Dasypyrum villosum(Dv) intergeneric hybridization. All lines, which showed several phenotypic differences compared to CS, had the same chromosome number (2n = 42) and structure as CS, and neither chromosomes nor chromatin from Dv were apparently added to their complement. However, Feulgen/DNA cytophotometry showed that there was more nuclear DNA in the lines than in the parental wheat (by 1.85%, 2.76%, and 1.26% in CS×V58, CS×V59, and CS×V60, respectively). Molecular investigation indicated the presence of Dv DNA in the ILs. AFLP analysis of genomic DNA from the ILs, CS, and Dv detected a total of 120 polymorphic bands, of which 7 (5.8%) were present in some or all the ILs and Dv but were absent in CS. PCR amplification, sequence analysis of amplicons, and Southern blot hybridization confirmed the presence of Dv-specific sequences in each of the ILs. These results indicate cryptic introgression of Dv DNA sequences into the genome of the ILs. Some implications of this finding are discussed. SN - 1424-859X UR - https://www.unboundmedicine.com/medline/citation/22104134/Cryptic_introgression_of_Dasypyrum_villosum_parental_DNA_in_wheat_lines_derived_from_intergeneric_hybridization_ L2 - https://www.karger.com?DOI=10.1159/000334275 DB - PRIME DP - Unbound Medicine ER -