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Quantitative proteomic analysis of serum proteins in patients with Parkinson's disease using an isobaric tag for relative and absolute quantification labeling, two-dimensional liquid chromatography, and tandem mass spectrometry.
Analyst. 2012 Jan 21; 137(2):490-5.A

Abstract

Parkinson's disease (PD) is a common disease which occurs in aged people with chronic, progressive degenerative character of the central nervous system. Until now there is no effective treatment method in PD patients before they show obvious symptoms for prevention and early diagnosis. In order to find out early disease specific biomarkers, two-dimensional liquid chromatography-tandem mass spectrometry coupled with isobaric tags for relative and absolute quantification (iTRAQ) labeling was employed to quantitatively identify the differentially expressed proteins among the different disease progress types of PD. 26 proteins were differentially expressed in a total of 258 identified proteins by proteomic techniques. The expression level of eight proteins which included sero-transferrin and clusterin increased. The expression level of eighteen proteins which include complement component 4B, apolipoprotein A-I, α-2-antiplasmin and coagulation factor V decreased. Those proteins may be associated with oxidative stress, mitochondrial dysfunction, abnormal protein aggregation and inflammation. In this study, the expression level of apolipoprotein A-I decreased, particularly in the early stage of PD patients. This protein regulated not only the lipid metabolism in the central nervous system, but also influenced the deposition process of proteins which are involved in neural degenerative diseases, such as the pathogenesis of PD.

Authors+Show Affiliations

Hua Dong Hospital Affiliated to Fudan University, Shanghai, 200040, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22108571

Citation

Zhang, Xiaping, et al. "Quantitative Proteomic Analysis of Serum Proteins in Patients With Parkinson's Disease Using an Isobaric Tag for Relative and Absolute Quantification Labeling, Two-dimensional Liquid Chromatography, and Tandem Mass Spectrometry." The Analyst, vol. 137, no. 2, 2012, pp. 490-5.
Zhang X, Yin X, Yu H, et al. Quantitative proteomic analysis of serum proteins in patients with Parkinson's disease using an isobaric tag for relative and absolute quantification labeling, two-dimensional liquid chromatography, and tandem mass spectrometry. Analyst. 2012;137(2):490-5.
Zhang, X., Yin, X., Yu, H., Liu, X., Yang, F., Yao, J., Jin, H., & Yang, P. (2012). Quantitative proteomic analysis of serum proteins in patients with Parkinson's disease using an isobaric tag for relative and absolute quantification labeling, two-dimensional liquid chromatography, and tandem mass spectrometry. The Analyst, 137(2), 490-5. https://doi.org/10.1039/c1an15551b
Zhang X, et al. Quantitative Proteomic Analysis of Serum Proteins in Patients With Parkinson's Disease Using an Isobaric Tag for Relative and Absolute Quantification Labeling, Two-dimensional Liquid Chromatography, and Tandem Mass Spectrometry. Analyst. 2012 Jan 21;137(2):490-5. PubMed PMID: 22108571.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quantitative proteomic analysis of serum proteins in patients with Parkinson's disease using an isobaric tag for relative and absolute quantification labeling, two-dimensional liquid chromatography, and tandem mass spectrometry. AU - Zhang,Xiaping, AU - Yin,Xuefei, AU - Yu,Haihong, AU - Liu,Xiaohui, AU - Yang,Fengying, AU - Yao,Jun, AU - Jin,Hong, AU - Yang,Pengyuan, Y1 - 2011/11/23/ PY - 2011/11/24/entrez PY - 2011/11/24/pubmed PY - 2012/4/6/medline SP - 490 EP - 5 JF - The Analyst JO - Analyst VL - 137 IS - 2 N2 - Parkinson's disease (PD) is a common disease which occurs in aged people with chronic, progressive degenerative character of the central nervous system. Until now there is no effective treatment method in PD patients before they show obvious symptoms for prevention and early diagnosis. In order to find out early disease specific biomarkers, two-dimensional liquid chromatography-tandem mass spectrometry coupled with isobaric tags for relative and absolute quantification (iTRAQ) labeling was employed to quantitatively identify the differentially expressed proteins among the different disease progress types of PD. 26 proteins were differentially expressed in a total of 258 identified proteins by proteomic techniques. The expression level of eight proteins which included sero-transferrin and clusterin increased. The expression level of eighteen proteins which include complement component 4B, apolipoprotein A-I, α-2-antiplasmin and coagulation factor V decreased. Those proteins may be associated with oxidative stress, mitochondrial dysfunction, abnormal protein aggregation and inflammation. In this study, the expression level of apolipoprotein A-I decreased, particularly in the early stage of PD patients. This protein regulated not only the lipid metabolism in the central nervous system, but also influenced the deposition process of proteins which are involved in neural degenerative diseases, such as the pathogenesis of PD. SN - 1364-5528 UR - https://www.unboundmedicine.com/medline/citation/22108571/Quantitative_proteomic_analysis_of_serum_proteins_in_patients_with_Parkinson's_disease_using_an_isobaric_tag_for_relative_and_absolute_quantification_labeling_two_dimensional_liquid_chromatography_and_tandem_mass_spectrometry_ L2 - https://doi.org/10.1039/c1an15551b DB - PRIME DP - Unbound Medicine ER -