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Vascular changes in rat hippocampus following a high saturated fat and cholesterol diet.
J Cereb Blood Flow Metab 2012; 32(4):643-53JC

Abstract

The long-term effects of a diet rich in saturated fat and cholesterol on the hippocampus were evaluated in this study. It has previously been shown that this type of diet is detrimental to health, particularly affecting peripheral organs such as the heart and liver. However, effects on the brain have not been fully evaluated. This study focused on the hippocampus, a brain region instrumental for learning and memory and vulnerable to ischemic damage. Reduced blood-brain barrier (BBB) integrity and increased microgliosis were observed in the hippocampus of rats fed a high-saturated-fat and cholesterol (HFHC) diet for 6 months. Interestingly, an increase in hippocampal protein levels of occludin, a tight junction protein, was found in HFHC-treated rats as well. Further investigation revealed decreased expression of the occludin protein in blood vessels and increased expression in the dentate gyrus hilar neurons and mossy fibers of the hippocampal cornus ammonis 3 in HFHC-treated rats. Our results show alterations in BBB integrity and expression of tight junction proteins after long-term exposure to HFHC diet in rats. These findings may suggest a biologic mechanism for previously observed behavioral deficits occurring in rats fed this diet.

Authors+Show Affiliations

Department of Neurosciences and the Center on Aging, Medical University of South Carolina, Charleston, South Carolina 29425, USA. freemal@musc.eduNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22108721

Citation

Freeman, Linnea R., and Ann-Charlotte E. Granholm. "Vascular Changes in Rat Hippocampus Following a High Saturated Fat and Cholesterol Diet." Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism, vol. 32, no. 4, 2012, pp. 643-53.
Freeman LR, Granholm AC. Vascular changes in rat hippocampus following a high saturated fat and cholesterol diet. J Cereb Blood Flow Metab. 2012;32(4):643-53.
Freeman, L. R., & Granholm, A. C. (2012). Vascular changes in rat hippocampus following a high saturated fat and cholesterol diet. Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism, 32(4), pp. 643-53. doi:10.1038/jcbfm.2011.168.
Freeman LR, Granholm AC. Vascular Changes in Rat Hippocampus Following a High Saturated Fat and Cholesterol Diet. J Cereb Blood Flow Metab. 2012;32(4):643-53. PubMed PMID: 22108721.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Vascular changes in rat hippocampus following a high saturated fat and cholesterol diet. AU - Freeman,Linnea R, AU - Granholm,Ann-Charlotte E, Y1 - 2011/11/23/ PY - 2011/11/24/entrez PY - 2011/11/24/pubmed PY - 2012/5/26/medline SP - 643 EP - 53 JF - Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism JO - J. Cereb. Blood Flow Metab. VL - 32 IS - 4 N2 - The long-term effects of a diet rich in saturated fat and cholesterol on the hippocampus were evaluated in this study. It has previously been shown that this type of diet is detrimental to health, particularly affecting peripheral organs such as the heart and liver. However, effects on the brain have not been fully evaluated. This study focused on the hippocampus, a brain region instrumental for learning and memory and vulnerable to ischemic damage. Reduced blood-brain barrier (BBB) integrity and increased microgliosis were observed in the hippocampus of rats fed a high-saturated-fat and cholesterol (HFHC) diet for 6 months. Interestingly, an increase in hippocampal protein levels of occludin, a tight junction protein, was found in HFHC-treated rats as well. Further investigation revealed decreased expression of the occludin protein in blood vessels and increased expression in the dentate gyrus hilar neurons and mossy fibers of the hippocampal cornus ammonis 3 in HFHC-treated rats. Our results show alterations in BBB integrity and expression of tight junction proteins after long-term exposure to HFHC diet in rats. These findings may suggest a biologic mechanism for previously observed behavioral deficits occurring in rats fed this diet. SN - 1559-7016 UR - https://www.unboundmedicine.com/medline/citation/22108721/Vascular_changes_in_rat_hippocampus_following_a_high_saturated_fat_and_cholesterol_diet_ L2 - http://journals.sagepub.com/doi/full/10.1038/jcbfm.2011.168?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -