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Genotoxicity of diuron and glyphosate in oyster spermatozoa and embryos.
Aquat Toxicol. 2012 Jan 15; 106-107:104-13.AT

Abstract

We investigated the effects of genotoxicant exposure in gametes and embryos to find a possible link between genotoxicity and reproduction/developmental impairment, and explore the impact of chemical genotoxicity on population dynamics. Our study focused on the genotoxic effects of two herbicides on oyster gametes and embryos: glyphosate (both as an active substance and in the Roundup formulation) and diuron. France is Europe's leading consumer of agrochemical substances and as such, contamination of France's coastal waters by pesticides is a major concern. Glyphosate and diuron are among the most frequently detected herbicides in oyster production areas; as oyster is a specie with external reproduction, its gametes and embryos are in direct contact with the surrounding waters and are hence particularly exposed to these potentially dangerous substances. In the course of this study, differences in genotoxic and embryotoxic responses were observed in the various experiments, possibly due to differences in pollutant sensitivity between the tested genitor lots. Glyphosate and Roundup had no effect on oyster development at the concentrations tested, whereas diuron significantly affected embryo-larval development from the lowest tested concentration of 0.05 μg L⁻¹, i.e. an environmentally realistic concentration. Diuron may therefore have a significant impact on oyster recruitment rates in the natural environment. Our spermiotoxicity study revealed none of the tested herbicides to be cytotoxic for oyster spermatozoa. However, the alkaline comet assay showed diuron to have a significant genotoxic effect on oyster spermatozoa at concentrations of 0.05 μg L⁻¹ upwards. Conversely, no effects due to diuron exposure were observed on sperm mitochondrial function or acrosomal membrane integrity. Although our initial results showed no negative effect on sperm function, the possible impact on fertilization rate and the consequences of the transmission of damaged DNA for oyster development and physiological performances, requires further investigation. A likely hypothesis to explain the embryotoxic and genotoxic effects of diuron is that it may act via causing oxidative stress.

Authors+Show Affiliations

Ifremer, Department of Biogeochemistry and Ecotoxicology, Laboratory of Ecotoxicology, Rue de l'Ile d'Yeu, BP 21105, 44311 Nantes Cedex 03, France. fakcha@ifremer.frNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22115909

Citation

Akcha, F, et al. "Genotoxicity of Diuron and Glyphosate in Oyster Spermatozoa and Embryos." Aquatic Toxicology (Amsterdam, Netherlands), vol. 106-107, 2012, pp. 104-13.
Akcha F, Spagnol C, Rouxel J. Genotoxicity of diuron and glyphosate in oyster spermatozoa and embryos. Aquat Toxicol. 2012;106-107:104-13.
Akcha, F., Spagnol, C., & Rouxel, J. (2012). Genotoxicity of diuron and glyphosate in oyster spermatozoa and embryos. Aquatic Toxicology (Amsterdam, Netherlands), 106-107, 104-13. https://doi.org/10.1016/j.aquatox.2011.10.018
Akcha F, Spagnol C, Rouxel J. Genotoxicity of Diuron and Glyphosate in Oyster Spermatozoa and Embryos. Aquat Toxicol. 2012 Jan 15;106-107:104-13. PubMed PMID: 22115909.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genotoxicity of diuron and glyphosate in oyster spermatozoa and embryos. AU - Akcha,F, AU - Spagnol,C, AU - Rouxel,J, Y1 - 2011/11/03/ PY - 2011/07/22/received PY - 2011/10/21/revised PY - 2011/10/25/accepted PY - 2011/11/26/entrez PY - 2011/11/26/pubmed PY - 2012/3/16/medline SP - 104 EP - 13 JF - Aquatic toxicology (Amsterdam, Netherlands) JO - Aquat Toxicol VL - 106-107 N2 - We investigated the effects of genotoxicant exposure in gametes and embryos to find a possible link between genotoxicity and reproduction/developmental impairment, and explore the impact of chemical genotoxicity on population dynamics. Our study focused on the genotoxic effects of two herbicides on oyster gametes and embryos: glyphosate (both as an active substance and in the Roundup formulation) and diuron. France is Europe's leading consumer of agrochemical substances and as such, contamination of France's coastal waters by pesticides is a major concern. Glyphosate and diuron are among the most frequently detected herbicides in oyster production areas; as oyster is a specie with external reproduction, its gametes and embryos are in direct contact with the surrounding waters and are hence particularly exposed to these potentially dangerous substances. In the course of this study, differences in genotoxic and embryotoxic responses were observed in the various experiments, possibly due to differences in pollutant sensitivity between the tested genitor lots. Glyphosate and Roundup had no effect on oyster development at the concentrations tested, whereas diuron significantly affected embryo-larval development from the lowest tested concentration of 0.05 μg L⁻¹, i.e. an environmentally realistic concentration. Diuron may therefore have a significant impact on oyster recruitment rates in the natural environment. Our spermiotoxicity study revealed none of the tested herbicides to be cytotoxic for oyster spermatozoa. However, the alkaline comet assay showed diuron to have a significant genotoxic effect on oyster spermatozoa at concentrations of 0.05 μg L⁻¹ upwards. Conversely, no effects due to diuron exposure were observed on sperm mitochondrial function or acrosomal membrane integrity. Although our initial results showed no negative effect on sperm function, the possible impact on fertilization rate and the consequences of the transmission of damaged DNA for oyster development and physiological performances, requires further investigation. A likely hypothesis to explain the embryotoxic and genotoxic effects of diuron is that it may act via causing oxidative stress. SN - 1879-1514 UR - https://www.unboundmedicine.com/medline/citation/22115909/Genotoxicity_of_diuron_and_glyphosate_in_oyster_spermatozoa_and_embryos_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0166-445X(11)00302-X DB - PRIME DP - Unbound Medicine ER -