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Antibacterial and antioxidant activities of hydroalcoholic stem bark extract of Schotia latifolia Jacq.
Asian Pac J Trop Med. 2011 Dec; 4(12):952-8.AP

Abstract

OBJECTIVE

To investigate the antibacterial and antioxidant activities of hydroalcoholic extract of Schotia latifolia (S. latifolia) bark commonly used in South Africa traditional medicine for the treatment of various ailments.

METHODS

The antibacterial test and MIC was determined by using agar well diffusion and dilution methods respectively against eight strains of bacteria. The total phenol, proanthocyanidin and flavonoid contents of S. latifolia were assessed using standard methods. The antioxidant activity of the extract was evaluated using ferric reducing power and the free radical scavenging activity against 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic-acid (ABTS), nitric oxide (NO), hydrogen peroxide (H(2)O(2)) and lipid oxidation (LO).

RESULTS

The antibacterial activity demonstrated an appreciable effect against all the gram positive bacteria at MIC between 0.016 and 10 mg/mL while that of gram negative bacteria was above 10 mg/mL. The plant extract exhibited high concentration of proanthocyanidin [(300.00±0.10) mg CE/g], followed by flavonoid [(12.46±0.04 mg) TE/g] and phenol [(11.06±0.03) mg QE/g] contents. Similarly, the extract at 0.5 mg/mL scavenges DPPH, ABTS, H(2)O(2), LO and NO by 87.55%, 89.47%, 77.15%, 86.48% and 77.75% of the radicals respectively. The reducing power was also found to be concentration dependent.

CONCLUSIONS

Our data suggest that S. latifolia extract has antibacterial and antioxidants activity and thus could be used as alternative therapy against antibiotic resistance bacteria and to prevent many radical related diseases.

Authors+Show Affiliations

School of Biological Sciences, University of Fort Hare, Alice, South Africa. sivanusdemi@gmail.comNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22118030

Citation

Oyedemi, Sunday Oyewole, and Anthony Jide Afolayan. "Antibacterial and Antioxidant Activities of Hydroalcoholic Stem Bark Extract of Schotia Latifolia Jacq." Asian Pacific Journal of Tropical Medicine, vol. 4, no. 12, 2011, pp. 952-8.
Oyedemi SO, Afolayan AJ. Antibacterial and antioxidant activities of hydroalcoholic stem bark extract of Schotia latifolia Jacq. Asian Pac J Trop Med. 2011;4(12):952-8.
Oyedemi, S. O., & Afolayan, A. J. (2011). Antibacterial and antioxidant activities of hydroalcoholic stem bark extract of Schotia latifolia Jacq. Asian Pacific Journal of Tropical Medicine, 4(12), 952-8. https://doi.org/10.1016/S1995-7645(11)60225-3
Oyedemi SO, Afolayan AJ. Antibacterial and Antioxidant Activities of Hydroalcoholic Stem Bark Extract of Schotia Latifolia Jacq. Asian Pac J Trop Med. 2011;4(12):952-8. PubMed PMID: 22118030.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antibacterial and antioxidant activities of hydroalcoholic stem bark extract of Schotia latifolia Jacq. AU - Oyedemi,Sunday Oyewole, AU - Afolayan,Anthony Jide, PY - 2011/07/11/received PY - 2011/08/15/revised PY - 2011/11/15/accepted PY - 2011/11/29/entrez PY - 2011/11/29/pubmed PY - 2012/2/14/medline SP - 952 EP - 8 JF - Asian Pacific journal of tropical medicine JO - Asian Pac J Trop Med VL - 4 IS - 12 N2 - OBJECTIVE: To investigate the antibacterial and antioxidant activities of hydroalcoholic extract of Schotia latifolia (S. latifolia) bark commonly used in South Africa traditional medicine for the treatment of various ailments. METHODS: The antibacterial test and MIC was determined by using agar well diffusion and dilution methods respectively against eight strains of bacteria. The total phenol, proanthocyanidin and flavonoid contents of S. latifolia were assessed using standard methods. The antioxidant activity of the extract was evaluated using ferric reducing power and the free radical scavenging activity against 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic-acid (ABTS), nitric oxide (NO), hydrogen peroxide (H(2)O(2)) and lipid oxidation (LO). RESULTS: The antibacterial activity demonstrated an appreciable effect against all the gram positive bacteria at MIC between 0.016 and 10 mg/mL while that of gram negative bacteria was above 10 mg/mL. The plant extract exhibited high concentration of proanthocyanidin [(300.00±0.10) mg CE/g], followed by flavonoid [(12.46±0.04 mg) TE/g] and phenol [(11.06±0.03) mg QE/g] contents. Similarly, the extract at 0.5 mg/mL scavenges DPPH, ABTS, H(2)O(2), LO and NO by 87.55%, 89.47%, 77.15%, 86.48% and 77.75% of the radicals respectively. The reducing power was also found to be concentration dependent. CONCLUSIONS: Our data suggest that S. latifolia extract has antibacterial and antioxidants activity and thus could be used as alternative therapy against antibiotic resistance bacteria and to prevent many radical related diseases. SN - 2352-4146 UR - https://www.unboundmedicine.com/medline/citation/22118030/Antibacterial_and_antioxidant_activities_of_hydroalcoholic_stem_bark_extract_of_Schotia_latifolia_Jacq_ DB - PRIME DP - Unbound Medicine ER -