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A rapid, sensitive and reliable diagnostic test for scrub typhus in China.
Indian J Med Microbiol. 2011 Oct-Dec; 29(4):368-71.IJ

Abstract

PURPOSE

To evaluate the performances for detection of IgM and IgG antibodies to Orientia. tsutsugamushi (Ot) using a gold conjugate-based rapid diagnostic test (RDT).

MATERIALS AND METHODS

The RDT employing mixture recombinant 56-kDa proteins of O. tsutsugamushi and the mIFA assay was performed on 33 patients from Fujian and Yunnan province respectively and 94 positive sera (36 from Hainan province and 58 from Jiangsu province) from convalescent stages of the patients with scrub typhus respectively and 82 negative sera from healthy farmers from Anhui province and Beijing City respectively in 2009. A comparison of the RDT and mIFA assay was performed by using the χ(2) test and the P level of ≤ 0.05 was considered to be significant.

RESULTS

Among these 94 positive sera from convalescent stages of the illness and 82 sera from control farmers, the specificity of RDT was 100% for both IgM and IgG tests. In 33 cases with scrub typhus, 5 cases were positively detected earlier by RDT than by mIFA for the IgM test, and 2 cases were positive for the IgG test. The sensitivities of RDT were 93.9% and 90.9% for IgM and IgG, respectively. Considering IgM and IgG together, the sensitivity was 100%. The geometric mean titre (GMT) of IFA and the RDT assay in diluted sera from confirmed cases were 1:37 versus 1:113 respectively (P<0.001) for IgM test and 1:99 versus 1:279 respectively (P<0.016) for IgG.

CONCLUSIONS

The RDT was more sensitive than the traditional IFA for the early diagnosis of scrub typhus and was particularly suitable for use in rural areas.

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Authors+Show Affiliations

Lijuan Z
Department of Rickettsiology, National Institute of Communicable Disease Control and Prevention, China CDC, Changping PO Box 5, Beijing 102206, China. zhanglijuan@icdc.cn
Si H
No affiliation info available
Yuming J
No affiliation info available
Liang L
No affiliation info available
Xuemei L
No affiliation info available
Lianying L
No affiliation info available
Huilan Y
No affiliation info available
Qiang Y
No affiliation info available
Chuangfu C
No affiliation info available
Shiwen W
No affiliation info available

MeSH

AdolescentAdultAgedAntibodies, BacterialChildChinaDiagnostic Tests, RoutineFemaleHumansImmunoassayImmunoglobulin GImmunoglobulin MMaleMiddle AgedOrientia tsutsugamushiScrub TyphusSensitivity and SpecificityYoung Adult

Pub Type(s)

Comparative Study
Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22120796

Citation

Lijuan, Zhang, et al. "A Rapid, Sensitive and Reliable Diagnostic Test for Scrub Typhus in China." Indian Journal of Medical Microbiology, vol. 29, no. 4, 2011, pp. 368-71.
Lijuan Z, Si H, Yuming J, et al. A rapid, sensitive and reliable diagnostic test for scrub typhus in China. Indian J Med Microbiol. 2011;29(4):368-71.
Lijuan, Z., Si, H., Yuming, J., Liang, L., Xuemei, L., Lianying, L., Huilan, Y., Qiang, Y., Chuangfu, C., & Shiwen, W. (2011). A rapid, sensitive and reliable diagnostic test for scrub typhus in China. Indian Journal of Medical Microbiology, 29(4), 368-71. https://doi.org/10.4103/0255-0857.90166
Lijuan Z, et al. A Rapid, Sensitive and Reliable Diagnostic Test for Scrub Typhus in China. Indian J Med Microbiol. 2011 Oct-Dec;29(4):368-71. PubMed PMID: 22120796.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A rapid, sensitive and reliable diagnostic test for scrub typhus in China. AU - Lijuan,Zhang, AU - Si,He, AU - Yuming,Jin, AU - Liang,Li, AU - Xuemei,Li, AU - Lianying,Liu, AU - Huilan,Yu, AU - Qiang,Yu, AU - Chuangfu,Chen, AU - Shiwen,Wang, PY - 2011/11/29/entrez PY - 2011/11/29/pubmed PY - 2012/3/16/medline SP - 368 EP - 71 JF - Indian journal of medical microbiology JO - Indian J Med Microbiol VL - 29 IS - 4 N2 - PURPOSE: To evaluate the performances for detection of IgM and IgG antibodies to Orientia. tsutsugamushi (Ot) using a gold conjugate-based rapid diagnostic test (RDT). MATERIALS AND METHODS: The RDT employing mixture recombinant 56-kDa proteins of O. tsutsugamushi and the mIFA assay was performed on 33 patients from Fujian and Yunnan province respectively and 94 positive sera (36 from Hainan province and 58 from Jiangsu province) from convalescent stages of the patients with scrub typhus respectively and 82 negative sera from healthy farmers from Anhui province and Beijing City respectively in 2009. A comparison of the RDT and mIFA assay was performed by using the χ(2) test and the P level of ≤ 0.05 was considered to be significant. RESULTS: Among these 94 positive sera from convalescent stages of the illness and 82 sera from control farmers, the specificity of RDT was 100% for both IgM and IgG tests. In 33 cases with scrub typhus, 5 cases were positively detected earlier by RDT than by mIFA for the IgM test, and 2 cases were positive for the IgG test. The sensitivities of RDT were 93.9% and 90.9% for IgM and IgG, respectively. Considering IgM and IgG together, the sensitivity was 100%. The geometric mean titre (GMT) of IFA and the RDT assay in diluted sera from confirmed cases were 1:37 versus 1:113 respectively (P<0.001) for IgM test and 1:99 versus 1:279 respectively (P<0.016) for IgG. CONCLUSIONS: The RDT was more sensitive than the traditional IFA for the early diagnosis of scrub typhus and was particularly suitable for use in rural areas. SN - 1998-3646 UR - https://www.unboundmedicine.com/medline/citation/22120796/A_rapid_sensitive_and_reliable_diagnostic_test_for_scrub_typhus_in_China_ L2 - http://www.ijmm.org/article.asp?issn=0255-0857;year=2011;volume=29;issue=4;spage=368;epage=371;aulast=Lijuan DB - PRIME DP - Unbound Medicine ER -