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Apoptosis-mediated inhibition of human breast cancer cell proliferation by lemon citrus extract.
Asian Pac J Cancer Prev. 2011; 12(6):1555-9.AP

Abstract

Dietary phytochemicals have a variety of antitumor properties. In the present study, the antitumor activity of methanolic extract of lemon fruit (lemon extract; LE) (LE) on the MCF-7 breast cancer cell line was investigated in vitro. Apoptotic cell death was analyzed using the TUNEL assay. In addition, the apoptosis mediated by LE extract in the MCF-7 cells was associated with the increased expression of the tumor suppressor p53 and caspase-3. Additionally, the expression of a pro-apoptotic gene, bax, was increased, and the expression of an anti-apoptotic gene, bcl-2, was decreased by LE extract treatment, resulting in a shift in the Bax:Bcl-2 ratio to one that favored apoptosis. The expression of a major apoptotic gene, caspase-3, was increased by LE extract treatment. In light of the above results, we concluded that LE extract can induce the apoptosis of MCF-7 breast cancer cells via Bax-related caspase-3 activation. This study provides experimental data that are relevant to the possible future clinical use of LE to treat breast cancer.

Authors+Show Affiliations

Molecular Cancer Biology Research Lab, Department of Food Sciences and Nutrition, College of Food and Agricultural Sciences, King Saud University, Riyadh, Saudi Arabia. alialshatwi@gmail.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22126498

Citation

Alshatwi, Ali A., et al. "Apoptosis-mediated Inhibition of Human Breast Cancer Cell Proliferation By Lemon Citrus Extract." Asian Pacific Journal of Cancer Prevention : APJCP, vol. 12, no. 6, 2011, pp. 1555-9.
Alshatwi AA, Shafi G, Hasan TN, et al. Apoptosis-mediated inhibition of human breast cancer cell proliferation by lemon citrus extract. Asian Pac J Cancer Prev. 2011;12(6):1555-9.
Alshatwi, A. A., Shafi, G., Hasan, T. N., Al-Hazzani, A. A., Alsaif, M. A., Alfawaz, M. A., Lei, K. Y., & Munshi, A. (2011). Apoptosis-mediated inhibition of human breast cancer cell proliferation by lemon citrus extract. Asian Pacific Journal of Cancer Prevention : APJCP, 12(6), 1555-9.
Alshatwi AA, et al. Apoptosis-mediated Inhibition of Human Breast Cancer Cell Proliferation By Lemon Citrus Extract. Asian Pac J Cancer Prev. 2011;12(6):1555-9. PubMed PMID: 22126498.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Apoptosis-mediated inhibition of human breast cancer cell proliferation by lemon citrus extract. AU - Alshatwi,Ali A, AU - Shafi,Gowhar, AU - Hasan,Tarique N, AU - Al-Hazzani,Amal A, AU - Alsaif,Mohammed A, AU - Alfawaz,Mohammed A, AU - Lei,K Y, AU - Munshi,Anjana, PY - 2011/12/1/entrez PY - 2011/12/1/pubmed PY - 2012/7/24/medline SP - 1555 EP - 9 JF - Asian Pacific journal of cancer prevention : APJCP JO - Asian Pac J Cancer Prev VL - 12 IS - 6 N2 - Dietary phytochemicals have a variety of antitumor properties. In the present study, the antitumor activity of methanolic extract of lemon fruit (lemon extract; LE) (LE) on the MCF-7 breast cancer cell line was investigated in vitro. Apoptotic cell death was analyzed using the TUNEL assay. In addition, the apoptosis mediated by LE extract in the MCF-7 cells was associated with the increased expression of the tumor suppressor p53 and caspase-3. Additionally, the expression of a pro-apoptotic gene, bax, was increased, and the expression of an anti-apoptotic gene, bcl-2, was decreased by LE extract treatment, resulting in a shift in the Bax:Bcl-2 ratio to one that favored apoptosis. The expression of a major apoptotic gene, caspase-3, was increased by LE extract treatment. In light of the above results, we concluded that LE extract can induce the apoptosis of MCF-7 breast cancer cells via Bax-related caspase-3 activation. This study provides experimental data that are relevant to the possible future clinical use of LE to treat breast cancer. SN - 2476-762X UR - https://www.unboundmedicine.com/medline/citation/22126498/full_citation/Apoptosis_mediated_inhibition_of_human_breast_cancer_cell_proliferation_by_lemon_citrus_extract_ L2 - http://journal.waocp.org/?sid=Entrez:PubMed&id=pmid:22126498&key=2011.12.6.1555 DB - PRIME DP - Unbound Medicine ER -