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[Changes of death receptor regulator expression in the articular cartilage of patients with Kashin-Beck disease].
Nan Fang Yi Ke Da Xue Xue Bao. 2011 Nov; 31(11):1851-4.NF

Abstract

OBJECTIVE

To investigate the changes in the expressions of Fas-associated death domain protein (FADD) and cellular-FLICE inhibitory protein (c-FLIP) in the articular cartilage of patients with Kashin-Beck disease (KBD) and the role of these proteins in the pathogenesis of KBD.

METHODS

The cartilage samples were collected from patients with established diagnosis of KBD and osteoarthritis and from healthy control subjects undergoing amputation due to traffic accidents. The expressions of Fas-associated death domain protein (FADD) and cellular-FLICE inhibitory protein (c-FLIP) in the cartilage were detected by immunohistochemistry, and the positive chondrocytes were counted in different layers of the articular cartilage under microscope.

RESULTS

The positivity rates of FADD in the middle layer of articular cartilage from patients with KBD [(28.68∓2.19)%] and osteoarthritis [(35.40∓2.34)%] were significantly higher than that in normal cartilage [(10.51∓5.02)%, F=16.245, P=0.000], but the rates in the upper and deeper layers were comparable among the 3 groups (P=0.206-0.761). In KBD cartilage, FADD expression was the highest in the middle layer [(28.68∓5.38)%] followed by the deeper layer [(17.94∓8.38)%]. Compared with the healthy controls, KBD and osteoarthritis patients showed significantly higher FLIP expression in the upper layer of the cartilage (F=5.929, P=0.018) but similar expressions in middle and deeper layers.

CONCLUSIONS

KBD patients have significant increased FADD expression in the middle layer but decreased FLIP expression in the upper layer of the cartilage, suggesting that the death receptor pathway and its regulators play important roles in the pathogenesis of KBD.

Authors+Show Affiliations

Faculty of Public Health, College of Medicine, Key Laboratory of Environment and Gene Related Diseases of Ministry Education, Key Laboratory of Trace Elements and Endemic Diseases of Ministry of Health, First Affiliated Hospital, Xi an Jiaotong University College of Medicine, Xi an 710061, China. wushixun313@stu.xjtu.edu.cnNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

22126763

Citation

Wu, Shi-xun, et al. "[Changes of Death Receptor Regulator Expression in the Articular Cartilage of Patients With Kashin-Beck Disease]." Nan Fang Yi Ke Da Xue Xue Bao = Journal of Southern Medical University, vol. 31, no. 11, 2011, pp. 1851-4.
Wu SX, Guo X, Liu JT, et al. [Changes of death receptor regulator expression in the articular cartilage of patients with Kashin-Beck disease]. Nan Fang Yi Ke Da Xue Xue Bao. 2011;31(11):1851-4.
Wu, S. X., Guo, X., Liu, J. T., Zhang, Z. T., Dennis, B. S., & Zhang, Y. G. (2011). [Changes of death receptor regulator expression in the articular cartilage of patients with Kashin-Beck disease]. Nan Fang Yi Ke Da Xue Xue Bao = Journal of Southern Medical University, 31(11), 1851-4.
Wu SX, et al. [Changes of Death Receptor Regulator Expression in the Articular Cartilage of Patients With Kashin-Beck Disease]. Nan Fang Yi Ke Da Xue Xue Bao. 2011;31(11):1851-4. PubMed PMID: 22126763.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Changes of death receptor regulator expression in the articular cartilage of patients with Kashin-Beck disease]. AU - Wu,Shi-xun, AU - Guo,Xiong, AU - Liu,Jiang-tao, AU - Zhang,Zeng-tie, AU - Dennis,Bannel S, AU - Zhang,Yin-gang, PY - 2011/12/1/entrez PY - 2011/12/1/pubmed PY - 2013/5/7/medline SP - 1851 EP - 4 JF - Nan fang yi ke da xue xue bao = Journal of Southern Medical University JO - Nan Fang Yi Ke Da Xue Xue Bao VL - 31 IS - 11 N2 - OBJECTIVE: To investigate the changes in the expressions of Fas-associated death domain protein (FADD) and cellular-FLICE inhibitory protein (c-FLIP) in the articular cartilage of patients with Kashin-Beck disease (KBD) and the role of these proteins in the pathogenesis of KBD. METHODS: The cartilage samples were collected from patients with established diagnosis of KBD and osteoarthritis and from healthy control subjects undergoing amputation due to traffic accidents. The expressions of Fas-associated death domain protein (FADD) and cellular-FLICE inhibitory protein (c-FLIP) in the cartilage were detected by immunohistochemistry, and the positive chondrocytes were counted in different layers of the articular cartilage under microscope. RESULTS: The positivity rates of FADD in the middle layer of articular cartilage from patients with KBD [(28.68∓2.19)%] and osteoarthritis [(35.40∓2.34)%] were significantly higher than that in normal cartilage [(10.51∓5.02)%, F=16.245, P=0.000], but the rates in the upper and deeper layers were comparable among the 3 groups (P=0.206-0.761). In KBD cartilage, FADD expression was the highest in the middle layer [(28.68∓5.38)%] followed by the deeper layer [(17.94∓8.38)%]. Compared with the healthy controls, KBD and osteoarthritis patients showed significantly higher FLIP expression in the upper layer of the cartilage (F=5.929, P=0.018) but similar expressions in middle and deeper layers. CONCLUSIONS: KBD patients have significant increased FADD expression in the middle layer but decreased FLIP expression in the upper layer of the cartilage, suggesting that the death receptor pathway and its regulators play important roles in the pathogenesis of KBD. SN - 1673-4254 UR - https://www.unboundmedicine.com/medline/citation/22126763/[Changes_of_death_receptor_regulator_expression_in_the_articular_cartilage_of_patients_with_Kashin_Beck_disease]_ DB - PRIME DP - Unbound Medicine ER -