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Presence of glucose in dialyzing fluid and synthesis of selected lipoxygenase-derived eicosanoids during hemodialysis.
Int Urol Nephrol. 2012 Dec; 44(6):1799-804.IU

Abstract

PURPOSE

Active metabolites of arachidonic acid (AA), eicosanoids, are exerting a significant influence on renal homeostasis. In our recent paper, we demonstrated that high levels of lipoxygenase (LOX)-derived AA metabolites, hydroxyeicosatetraenoic acids (HETEs), unfavorably influence post-transplant function of kidney allografts. Hence, in this study, we wanted (1) to analyze 5-, 12- and 15-HETE levels in non-diabetic chronic kidney disease (CKD) patients, already undergoing regular hemodialysis treatment, and determine factors that may influence these eicosanoids' generation, as well as, (2) to verify whether application of glucose-containing, instead of glucose-free, dialyzing fluids may be beneficial for the limitation of 5-, 12- and 15-HETE synthesis during a single hemodialysis session.

METHODS

Twenty-four healthy individuals and 50 CKD patients undergoing regular hemodialysis treatment were included in the study. CKD patients were divided into two subgroups depending on presence/absence of glucose in dialyzing fluid. LOX-derived HETEs were measured using liquid chromatography.

RESULTS

Results demonstrated higher levels of examined eicosanoids in CKD patients (P<0.05 for all). Older age and higher C-reactive protein levels were associated with HETEs concentrations. Presence of glucose in dialyzing fluid significantly diminished the increase in 5- and 12-HETE synthesis (411.24% vs. 107.29%, P<0.006; 301.70% vs. 98.21%, P<0.0008, respectively), however, it did not influence 15-HETE generation (156.98% vs. 135.24%, P=0.26).

CONCLUSIONS

Non-diabetic CKD patients have higher levels of LOX-derived HETEs, which are associated mainly with age and intensified inflammatory process. The presence of glucose in the dialysate is associated with a reduced synthesis of selected LOX-derived HETEs in these patients; hence, we speculate that the application of such a simple hemodialysis fluid modification may have a favorable influence on post-transplant outcomes.

Authors+Show Affiliations

Department of Laboratory Diagnostics and Molecular Medicine, Pomeranian Medical University, Al. Powstancow Wlkp. 72, 70-111, Szczecin, Poland.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22127406

Citation

Dołęgowska, Barbara, et al. "Presence of Glucose in Dialyzing Fluid and Synthesis of Selected Lipoxygenase-derived Eicosanoids During Hemodialysis." International Urology and Nephrology, vol. 44, no. 6, 2012, pp. 1799-804.
Dołęgowska B, Błogowski W, Stępniewska J, et al. Presence of glucose in dialyzing fluid and synthesis of selected lipoxygenase-derived eicosanoids during hemodialysis. Int Urol Nephrol. 2012;44(6):1799-804.
Dołęgowska, B., Błogowski, W., Stępniewska, J., Safranow, K., Jakubowska, K., & Olszewska, M. (2012). Presence of glucose in dialyzing fluid and synthesis of selected lipoxygenase-derived eicosanoids during hemodialysis. International Urology and Nephrology, 44(6), 1799-804. https://doi.org/10.1007/s11255-011-0089-5
Dołęgowska B, et al. Presence of Glucose in Dialyzing Fluid and Synthesis of Selected Lipoxygenase-derived Eicosanoids During Hemodialysis. Int Urol Nephrol. 2012;44(6):1799-804. PubMed PMID: 22127406.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Presence of glucose in dialyzing fluid and synthesis of selected lipoxygenase-derived eicosanoids during hemodialysis. AU - Dołęgowska,Barbara, AU - Błogowski,Wojciech, AU - Stępniewska,Joanna, AU - Safranow,Krzysztof, AU - Jakubowska,Katarzyna, AU - Olszewska,Maria, Y1 - 2011/11/30/ PY - 2011/09/08/received PY - 2011/11/04/accepted PY - 2011/12/1/entrez PY - 2011/12/1/pubmed PY - 2013/5/15/medline SP - 1799 EP - 804 JF - International urology and nephrology JO - Int Urol Nephrol VL - 44 IS - 6 N2 - PURPOSE: Active metabolites of arachidonic acid (AA), eicosanoids, are exerting a significant influence on renal homeostasis. In our recent paper, we demonstrated that high levels of lipoxygenase (LOX)-derived AA metabolites, hydroxyeicosatetraenoic acids (HETEs), unfavorably influence post-transplant function of kidney allografts. Hence, in this study, we wanted (1) to analyze 5-, 12- and 15-HETE levels in non-diabetic chronic kidney disease (CKD) patients, already undergoing regular hemodialysis treatment, and determine factors that may influence these eicosanoids' generation, as well as, (2) to verify whether application of glucose-containing, instead of glucose-free, dialyzing fluids may be beneficial for the limitation of 5-, 12- and 15-HETE synthesis during a single hemodialysis session. METHODS: Twenty-four healthy individuals and 50 CKD patients undergoing regular hemodialysis treatment were included in the study. CKD patients were divided into two subgroups depending on presence/absence of glucose in dialyzing fluid. LOX-derived HETEs were measured using liquid chromatography. RESULTS: Results demonstrated higher levels of examined eicosanoids in CKD patients (P<0.05 for all). Older age and higher C-reactive protein levels were associated with HETEs concentrations. Presence of glucose in dialyzing fluid significantly diminished the increase in 5- and 12-HETE synthesis (411.24% vs. 107.29%, P<0.006; 301.70% vs. 98.21%, P<0.0008, respectively), however, it did not influence 15-HETE generation (156.98% vs. 135.24%, P=0.26). CONCLUSIONS: Non-diabetic CKD patients have higher levels of LOX-derived HETEs, which are associated mainly with age and intensified inflammatory process. The presence of glucose in the dialysate is associated with a reduced synthesis of selected LOX-derived HETEs in these patients; hence, we speculate that the application of such a simple hemodialysis fluid modification may have a favorable influence on post-transplant outcomes. SN - 1573-2584 UR - https://www.unboundmedicine.com/medline/citation/22127406/Presence_of_glucose_in_dialyzing_fluid_and_synthesis_of_selected_lipoxygenase_derived_eicosanoids_during_hemodialysis_ L2 - https://doi.org/10.1007/s11255-011-0089-5 DB - PRIME DP - Unbound Medicine ER -