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High prevalence of spa types associated with the clonal lineage CC398 among tetracycline-resistant methicillin-resistant Staphylococcus aureus strains in a Spanish hospital.
J Antimicrob Chemother. 2012 Feb; 67(2):330-4.JA

Abstract

OBJECTIVES

The clonal lineages, resistance mechanisms and virulence traits of tetracycline-resistant (Tet(R)) methicillin-resistant Staphylococcus aureus (MRSA) strains isolated in a Spanish hospital during 2009 and 2010 were investigated.

METHODS

Fifty-two Tet(R) MRSA strains from unrelated patients were included in this study. Susceptibility to 26 antimicrobial agents was determined and 24 resistance genes were tested for by PCR. The sequences of the genes grlA and gyrA were analysed in all ciprofloxacin-resistant MRSA isolates. For all strains, spa, SCCmec and agr typing was implemented. Multilocus sequence typing was performed for 16 representative strains of the different spa types. The presence of the genes tst, lukF/lukS-PV, eta, etb, etd and cna was investigated by PCR.

RESULTS

Fifteen different spa types, four of them new ones, were detected among the 52 strains, being associated with the following clonal complexes (CCs): CC398 (67.3%), CC1 (11.5%), CC5 (11.5%) and CC8 (9.6%). A novel sequence type (ST), ST2077, belonging to CC398 was identified. Most MRSA CC398 strains were typed as SCCmecV-agrI. In addition to β-lactam resistance, isolates showed resistance to (gene/number of strains): tetracycline [tet(K)/36, tet(L)/8 and tet(M)/48], macrolides and lincosamides [erm(B)/6, erm(C)/25, erm(T)/2, msr(A)/msr(B)/4 and mph(C)/4], aminoglycosides [aac(6')-Ie-aph(2')-Ia/8, ant(4')-Ia/13 and aph(3')-IIIa/15], trimethoprim [dfrS1/2 and dfrK/3] and mupirocin (mupA/3). Strains investigated for mutations mediating quinolone resistance revealed an S80F exchange in GrlA and different changes in GyrA. Three strains were Panton-Valentine leucocidin-positive (ST8 and ST94) and 41 strains were cna-positive. All MRSA isolates were negative for the genes tst, eta, etb and etd.

CONCLUSIONS

Tetracycline resistance could be a good marker for MRSA CC398, although this resistance can also be found in other lineages.

Authors+Show Affiliations

Área Bioquímica y Biología Molecular, Universidad de La Rioja, Logroño, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22127589

Citation

Lozano, Carmen, et al. "High Prevalence of Spa Types Associated With the Clonal Lineage CC398 Among Tetracycline-resistant Methicillin-resistant Staphylococcus Aureus Strains in a Spanish Hospital." The Journal of Antimicrobial Chemotherapy, vol. 67, no. 2, 2012, pp. 330-4.
Lozano C, Rezusta A, Gómez P, et al. High prevalence of spa types associated with the clonal lineage CC398 among tetracycline-resistant methicillin-resistant Staphylococcus aureus strains in a Spanish hospital. J Antimicrob Chemother. 2012;67(2):330-4.
Lozano, C., Rezusta, A., Gómez, P., Gómez-Sanz, E., Báez, N., Martin-Saco, G., Zarazaga, M., & Torres, C. (2012). High prevalence of spa types associated with the clonal lineage CC398 among tetracycline-resistant methicillin-resistant Staphylococcus aureus strains in a Spanish hospital. The Journal of Antimicrobial Chemotherapy, 67(2), 330-4. https://doi.org/10.1093/jac/dkr497
Lozano C, et al. High Prevalence of Spa Types Associated With the Clonal Lineage CC398 Among Tetracycline-resistant Methicillin-resistant Staphylococcus Aureus Strains in a Spanish Hospital. J Antimicrob Chemother. 2012;67(2):330-4. PubMed PMID: 22127589.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - High prevalence of spa types associated with the clonal lineage CC398 among tetracycline-resistant methicillin-resistant Staphylococcus aureus strains in a Spanish hospital. AU - Lozano,Carmen, AU - Rezusta,Antonio, AU - Gómez,Paula, AU - Gómez-Sanz,Elena, AU - Báez,Natalia, AU - Martin-Saco,Gloria, AU - Zarazaga,Myriam, AU - Torres,Carmen, Y1 - 2011/11/28/ PY - 2011/12/1/entrez PY - 2011/12/1/pubmed PY - 2012/5/2/medline SP - 330 EP - 4 JF - The Journal of antimicrobial chemotherapy JO - J Antimicrob Chemother VL - 67 IS - 2 N2 - OBJECTIVES: The clonal lineages, resistance mechanisms and virulence traits of tetracycline-resistant (Tet(R)) methicillin-resistant Staphylococcus aureus (MRSA) strains isolated in a Spanish hospital during 2009 and 2010 were investigated. METHODS: Fifty-two Tet(R) MRSA strains from unrelated patients were included in this study. Susceptibility to 26 antimicrobial agents was determined and 24 resistance genes were tested for by PCR. The sequences of the genes grlA and gyrA were analysed in all ciprofloxacin-resistant MRSA isolates. For all strains, spa, SCCmec and agr typing was implemented. Multilocus sequence typing was performed for 16 representative strains of the different spa types. The presence of the genes tst, lukF/lukS-PV, eta, etb, etd and cna was investigated by PCR. RESULTS: Fifteen different spa types, four of them new ones, were detected among the 52 strains, being associated with the following clonal complexes (CCs): CC398 (67.3%), CC1 (11.5%), CC5 (11.5%) and CC8 (9.6%). A novel sequence type (ST), ST2077, belonging to CC398 was identified. Most MRSA CC398 strains were typed as SCCmecV-agrI. In addition to β-lactam resistance, isolates showed resistance to (gene/number of strains): tetracycline [tet(K)/36, tet(L)/8 and tet(M)/48], macrolides and lincosamides [erm(B)/6, erm(C)/25, erm(T)/2, msr(A)/msr(B)/4 and mph(C)/4], aminoglycosides [aac(6')-Ie-aph(2')-Ia/8, ant(4')-Ia/13 and aph(3')-IIIa/15], trimethoprim [dfrS1/2 and dfrK/3] and mupirocin (mupA/3). Strains investigated for mutations mediating quinolone resistance revealed an S80F exchange in GrlA and different changes in GyrA. Three strains were Panton-Valentine leucocidin-positive (ST8 and ST94) and 41 strains were cna-positive. All MRSA isolates were negative for the genes tst, eta, etb and etd. CONCLUSIONS: Tetracycline resistance could be a good marker for MRSA CC398, although this resistance can also be found in other lineages. SN - 1460-2091 UR - https://www.unboundmedicine.com/medline/citation/22127589/High_prevalence_of_spa_types_associated_with_the_clonal_lineage_CC398_among_tetracycline_resistant_methicillin_resistant_Staphylococcus_aureus_strains_in_a_Spanish_hospital_ L2 - https://academic.oup.com/jac/article-lookup/doi/10.1093/jac/dkr497 DB - PRIME DP - Unbound Medicine ER -