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Translocation of PEGylated quantum dots across rat alveolar epithelial cell monolayers.
Int J Nanomedicine. 2011; 6:2849-57.IJ

Abstract

BACKGROUND

In this study, primary rat alveolar epithelial cell monolayers (RAECM) were used to investigate transalveolar epithelial quantum dot trafficking rates and underlying transport mechanisms.

METHODS

Trafficking rates of quantum dots (PEGylated CdSe/ZnS, core size 5.3 nm, hydrodynamic size 25 nm) in the apical-to-basolateral direction across RAECM were determined. Changes in bioelectric properties (ie, transmonolayer resistance and equivalent active ion transport rate) of RAECM in the presence or absence of quantum dots were measured. Involvement of endocytic pathways in quantum dot trafficking across RAECM was assessed using specific inhibitors (eg, methyl-β-cyclodextrin, chlorpromazine, and dynasore for caveolin-, clathrin-, and dynamin-mediated endocytosis, respectively). The effects of lowering tight junctional resistance on quantum dot trafficking were determined by depleting Ca(2+) in apical and basolateral bathing fluids of RAECM using 2 mM EGTA. Effects of temperature on quantum dot trafficking were studied by lowering temperature from 37°C to 4°C.

RESULTS

Apical exposure of RAECM to quantum dots did not elicit changes in transmonolayer resistance or ion transport rate for up to 24 hours; quantum dot trafficking rates were not surface charge-dependent; methyl-β-cyclodextrin, chlorpromazine, and dynasore did not decrease quantum dot trafficking rates; lowering of temperature decreased transmonolayer resistance by approximately 90% with a concomitant increase in quantum dot trafficking by about 80%; and 24 hours of treatment of RAECM with EGTA decreased transmonolayer resistance by about 95%, with increased quantum dot trafficking of up to approximately 130%.

CONCLUSION

These data indicate that quantum dots do not injure RAECM and that quantum dot trafficking does not appear to take place via endocytic pathways involving caveolin, clathrin, or dynamin. We conclude that quantum dot translocation across RAECM takes place via both transcellular and paracellular pathways and, based on comparison with our prior studies, interactions of nanoparticles with RAECM are strongly dependent on nanoparticle composition and surface properties.

Authors+Show Affiliations

Will Rogers Institute Pulmonary Research Center, University of Southern California, Los Angeles, CA, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22131830

Citation

Fazlollahi, Farnoosh, et al. "Translocation of PEGylated Quantum Dots Across Rat Alveolar Epithelial Cell Monolayers." International Journal of Nanomedicine, vol. 6, 2011, pp. 2849-57.
Fazlollahi F, Sipos A, Kim YH, et al. Translocation of PEGylated quantum dots across rat alveolar epithelial cell monolayers. Int J Nanomedicine. 2011;6:2849-57.
Fazlollahi, F., Sipos, A., Kim, Y. H., Hamm-Alvarez, S. F., Borok, Z., Kim, K. J., & Crandall, E. D. (2011). Translocation of PEGylated quantum dots across rat alveolar epithelial cell monolayers. International Journal of Nanomedicine, 6, 2849-57. https://doi.org/10.2147/IJN.S26051
Fazlollahi F, et al. Translocation of PEGylated Quantum Dots Across Rat Alveolar Epithelial Cell Monolayers. Int J Nanomedicine. 2011;6:2849-57. PubMed PMID: 22131830.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Translocation of PEGylated quantum dots across rat alveolar epithelial cell monolayers. AU - Fazlollahi,Farnoosh, AU - Sipos,Arnold, AU - Kim,Yong Ho, AU - Hamm-Alvarez,Sarah F, AU - Borok,Zea, AU - Kim,Kwang-Jin, AU - Crandall,Edward D, Y1 - 2011/11/10/ PY - 2011/12/2/entrez PY - 2011/12/2/pubmed PY - 2012/6/2/medline KW - alveolar epithelial barrier KW - endocytosis KW - paracellular pathways KW - transport SP - 2849 EP - 57 JF - International journal of nanomedicine JO - Int J Nanomedicine VL - 6 N2 - BACKGROUND: In this study, primary rat alveolar epithelial cell monolayers (RAECM) were used to investigate transalveolar epithelial quantum dot trafficking rates and underlying transport mechanisms. METHODS: Trafficking rates of quantum dots (PEGylated CdSe/ZnS, core size 5.3 nm, hydrodynamic size 25 nm) in the apical-to-basolateral direction across RAECM were determined. Changes in bioelectric properties (ie, transmonolayer resistance and equivalent active ion transport rate) of RAECM in the presence or absence of quantum dots were measured. Involvement of endocytic pathways in quantum dot trafficking across RAECM was assessed using specific inhibitors (eg, methyl-β-cyclodextrin, chlorpromazine, and dynasore for caveolin-, clathrin-, and dynamin-mediated endocytosis, respectively). The effects of lowering tight junctional resistance on quantum dot trafficking were determined by depleting Ca(2+) in apical and basolateral bathing fluids of RAECM using 2 mM EGTA. Effects of temperature on quantum dot trafficking were studied by lowering temperature from 37°C to 4°C. RESULTS: Apical exposure of RAECM to quantum dots did not elicit changes in transmonolayer resistance or ion transport rate for up to 24 hours; quantum dot trafficking rates were not surface charge-dependent; methyl-β-cyclodextrin, chlorpromazine, and dynasore did not decrease quantum dot trafficking rates; lowering of temperature decreased transmonolayer resistance by approximately 90% with a concomitant increase in quantum dot trafficking by about 80%; and 24 hours of treatment of RAECM with EGTA decreased transmonolayer resistance by about 95%, with increased quantum dot trafficking of up to approximately 130%. CONCLUSION: These data indicate that quantum dots do not injure RAECM and that quantum dot trafficking does not appear to take place via endocytic pathways involving caveolin, clathrin, or dynamin. We conclude that quantum dot translocation across RAECM takes place via both transcellular and paracellular pathways and, based on comparison with our prior studies, interactions of nanoparticles with RAECM are strongly dependent on nanoparticle composition and surface properties. SN - 1178-2013 UR - https://www.unboundmedicine.com/medline/citation/22131830/Translocation_of_PEGylated_quantum_dots_across_rat_alveolar_epithelial_cell_monolayers_ L2 - https://dx.doi.org/10.2147/IJN.S26051 DB - PRIME DP - Unbound Medicine ER -