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In vitro antitumor activity and structure characterization of ethanol extracts from wild and cultivated Chaga medicinal mushroom, Inonotus obliquus (Pers.:Fr.) Pilát (Aphyllophoromycetideae).
Int J Med Mushrooms. 2011; 13(2):121-30.IJ

Abstract

Inonotus obliquus (Pers.:Fr.) Pilát has been traditionally used as a folk remedy for treatment of cancers, cardiovascular disease and diabetes in Russia, Poland, and most of the Baltic countries, but natural reserves of this fungus have nearly been exhausted. This study was designed to investigate the artificial cultivation of I. obliquus and the antitumor activity of its tissues. The ethanol extract of cultivated sclerotium had the highest cell growth inhibitory rate (74.6%) as determined by an 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. 78% of the bags produced sclerotia and only 6.17 g/bag of sclerotium was obtained. Extracts of the cultivated fruiting body showed 44.2% inhibitory activity against tumor cells. However, the yield was as high as 18.24 g/bag, and 98% of the bags produced fruiting body. The results of gas chromatography-mass spectroscopy (GC-MS) showed that similar compounds were extracted from the wild and cultivated samples. The principal compounds observed were lanosterol, inotodiol, and ergosterol. Their percentages of the mass fraction were 86.1, 59.9, and 71.8% of the total, for the wild sclerotium, cultivated sclerotium, and cultivated fruiting body, respectively. Ergosterol was found to be much higher (27.32%) in cultivated fruiting body. We conclude that cultivated fruiting body of I. obliquus obtained by inoculation of the substrate with spawn mycelium of the fifth generation could serve as an ideal substitute for the wild I. obliquus.

Authors+Show Affiliations

Laboratory for Biotechnology of Medicinal Plants of Jiangsu Province, Xuzhou Normal University, Xuzhou, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22135888

Citation

Sun, Yong, et al. "In Vitro Antitumor Activity and Structure Characterization of Ethanol Extracts From Wild and Cultivated Chaga Medicinal Mushroom, Inonotus Obliquus (Pers.:Fr.) Pilát (Aphyllophoromycetideae)." International Journal of Medicinal Mushrooms, vol. 13, no. 2, 2011, pp. 121-30.
Sun Y, Yin T, Chen XH, et al. In vitro antitumor activity and structure characterization of ethanol extracts from wild and cultivated Chaga medicinal mushroom, Inonotus obliquus (Pers.:Fr.) Pilát (Aphyllophoromycetideae). Int J Med Mushrooms. 2011;13(2):121-30.
Sun, Y., Yin, T., Chen, X. H., Zhang, G., Curtis, R. B., Lu, Z. H., & Jiang, J. H. (2011). In vitro antitumor activity and structure characterization of ethanol extracts from wild and cultivated Chaga medicinal mushroom, Inonotus obliquus (Pers.:Fr.) Pilát (Aphyllophoromycetideae). International Journal of Medicinal Mushrooms, 13(2), 121-30.
Sun Y, et al. In Vitro Antitumor Activity and Structure Characterization of Ethanol Extracts From Wild and Cultivated Chaga Medicinal Mushroom, Inonotus Obliquus (Pers.:Fr.) Pilát (Aphyllophoromycetideae). Int J Med Mushrooms. 2011;13(2):121-30. PubMed PMID: 22135888.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro antitumor activity and structure characterization of ethanol extracts from wild and cultivated Chaga medicinal mushroom, Inonotus obliquus (Pers.:Fr.) Pilát (Aphyllophoromycetideae). AU - Sun,Yong, AU - Yin,Ting, AU - Chen,Xian-Hui, AU - Zhang,Gong, AU - Curtis,Rempel B, AU - Lu,Zhan-Hui, AU - Jiang,Ji-Hong, PY - 2011/12/6/entrez PY - 2011/12/6/pubmed PY - 2012/2/15/medline SP - 121 EP - 30 JF - International journal of medicinal mushrooms JO - Int J Med Mushrooms VL - 13 IS - 2 N2 - Inonotus obliquus (Pers.:Fr.) Pilát has been traditionally used as a folk remedy for treatment of cancers, cardiovascular disease and diabetes in Russia, Poland, and most of the Baltic countries, but natural reserves of this fungus have nearly been exhausted. This study was designed to investigate the artificial cultivation of I. obliquus and the antitumor activity of its tissues. The ethanol extract of cultivated sclerotium had the highest cell growth inhibitory rate (74.6%) as determined by an 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. 78% of the bags produced sclerotia and only 6.17 g/bag of sclerotium was obtained. Extracts of the cultivated fruiting body showed 44.2% inhibitory activity against tumor cells. However, the yield was as high as 18.24 g/bag, and 98% of the bags produced fruiting body. The results of gas chromatography-mass spectroscopy (GC-MS) showed that similar compounds were extracted from the wild and cultivated samples. The principal compounds observed were lanosterol, inotodiol, and ergosterol. Their percentages of the mass fraction were 86.1, 59.9, and 71.8% of the total, for the wild sclerotium, cultivated sclerotium, and cultivated fruiting body, respectively. Ergosterol was found to be much higher (27.32%) in cultivated fruiting body. We conclude that cultivated fruiting body of I. obliquus obtained by inoculation of the substrate with spawn mycelium of the fifth generation could serve as an ideal substitute for the wild I. obliquus. SN - 1521-9437 UR - https://www.unboundmedicine.com/medline/citation/22135888/In_vitro_antitumor_activity_and_structure_characterization_of_ethanol_extracts_from_wild_and_cultivated_Chaga_medicinal_mushroom_Inonotus_obliquus__Pers_:Fr___Pilát__Aphyllophoromycetideae__ DB - PRIME DP - Unbound Medicine ER -