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Characteristics of intestinal lamina propria dendritic cells in a mouse model of postinfectious irritable bowel syndrome.
J Gastroenterol Hepatol. 2012 May; 27(5):935-44.JG

Abstract

BACKGROUND AND AIM

Postinfectious irritable bowel syndrome (PI-IBS), which results from inflammation has been emphasized a lot recently. Dendritic cells (DCs) may contribute to intestinal mucosal immune activation in the pathogenesis of PI-IBS. This study tested the hypothesis that phenotype and function of intestinal lamina propria DCs (LPDCs) changed in the development of a PI-IBS mouse model.

METHODS

Mice infected with Trichinella spiralis underwent abdominal withdrawal reflex (AWR) to evaluate visceral sensitivity. LPDCs were isolated and purified by intestine digestion and magnetic label-based technique. Surface markers were analyzed by flow cytometry. Endocytic activity, mixed lymphocyte reaction (MLR) and chemotaxis were studied. Cytokine production of the LPDCs cocultured with CD4(+) T cells was determined.

RESULTS

Intestinal inflammation resolved after 8 weeks infection with sustained visceral hyperalgesia. Surface markers CD86 and MHCII were lower in the acute infection group, but increased in the PI-IBS stage. Enhanced ability of endocytic activity and decreased abilities to attract and stimulate CD4(+) T cell proliferation were in the acute infection group. However, LPDCs in the PI-IBS stage showed weakened endocytic ability with enhanced abilities to attract and stimulate CD4(+) T cell proliferation. Cocultured LPDCs with CD4(+) T cells showed a predominant Th2 response in the acute infection stage, and more important roles of Th1, Th17 responses in the PI-IBS stage.

CONCLUSIONS

The hypothesis was supported that the phenotype and function of LPDCs changed in the development of PI-IBS, which induced the maintenance of intestinal mucosal immune activation and might provide a clue for the treatment of the disease.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Long Y
Division of Gastroenterology, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Wang W
No affiliation info available
Wang H
No affiliation info available
Hao L
No affiliation info available
Qian W
No affiliation info available
Hou X
No affiliation info available

MeSH

AnimalsB7-2 AntigenCD4-Positive T-LymphocytesCell ProliferationCells, CulturedChemotaxisCytokinesDendritic CellsHistocompatibility Antigens Class IIHyperalgesiaIntestinal MucosaIrritable Bowel SyndromeLymphocyte Culture Test, MixedMiceModels, AnimalPhenotypeReflex, AbdominalTrichinella spiralisTrichinellosis

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22141367

Citation

Long, Yanqin, et al. "Characteristics of Intestinal Lamina Propria Dendritic Cells in a Mouse Model of Postinfectious Irritable Bowel Syndrome." Journal of Gastroenterology and Hepatology, vol. 27, no. 5, 2012, pp. 935-44.
Long Y, Wang W, Wang H, et al. Characteristics of intestinal lamina propria dendritic cells in a mouse model of postinfectious irritable bowel syndrome. J Gastroenterol Hepatol. 2012;27(5):935-44.
Long, Y., Wang, W., Wang, H., Hao, L., Qian, W., & Hou, X. (2012). Characteristics of intestinal lamina propria dendritic cells in a mouse model of postinfectious irritable bowel syndrome. Journal of Gastroenterology and Hepatology, 27(5), 935-44. https://doi.org/10.1111/j.1440-1746.2011.07046.x
Long Y, et al. Characteristics of Intestinal Lamina Propria Dendritic Cells in a Mouse Model of Postinfectious Irritable Bowel Syndrome. J Gastroenterol Hepatol. 2012;27(5):935-44. PubMed PMID: 22141367.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characteristics of intestinal lamina propria dendritic cells in a mouse model of postinfectious irritable bowel syndrome. AU - Long,Yanqin, AU - Wang,Wenfeng, AU - Wang,Huan, AU - Hao,Liangcheng, AU - Qian,Wei, AU - Hou,Xiaohua, PY - 2011/12/7/entrez PY - 2011/12/7/pubmed PY - 2012/9/22/medline SP - 935 EP - 44 JF - Journal of gastroenterology and hepatology JO - J Gastroenterol Hepatol VL - 27 IS - 5 N2 - BACKGROUND AND AIM: Postinfectious irritable bowel syndrome (PI-IBS), which results from inflammation has been emphasized a lot recently. Dendritic cells (DCs) may contribute to intestinal mucosal immune activation in the pathogenesis of PI-IBS. This study tested the hypothesis that phenotype and function of intestinal lamina propria DCs (LPDCs) changed in the development of a PI-IBS mouse model. METHODS: Mice infected with Trichinella spiralis underwent abdominal withdrawal reflex (AWR) to evaluate visceral sensitivity. LPDCs were isolated and purified by intestine digestion and magnetic label-based technique. Surface markers were analyzed by flow cytometry. Endocytic activity, mixed lymphocyte reaction (MLR) and chemotaxis were studied. Cytokine production of the LPDCs cocultured with CD4(+) T cells was determined. RESULTS: Intestinal inflammation resolved after 8 weeks infection with sustained visceral hyperalgesia. Surface markers CD86 and MHCII were lower in the acute infection group, but increased in the PI-IBS stage. Enhanced ability of endocytic activity and decreased abilities to attract and stimulate CD4(+) T cell proliferation were in the acute infection group. However, LPDCs in the PI-IBS stage showed weakened endocytic ability with enhanced abilities to attract and stimulate CD4(+) T cell proliferation. Cocultured LPDCs with CD4(+) T cells showed a predominant Th2 response in the acute infection stage, and more important roles of Th1, Th17 responses in the PI-IBS stage. CONCLUSIONS: The hypothesis was supported that the phenotype and function of LPDCs changed in the development of PI-IBS, which induced the maintenance of intestinal mucosal immune activation and might provide a clue for the treatment of the disease. SN - 1440-1746 UR - https://www.unboundmedicine.com/medline/citation/22141367/Characteristics_of_intestinal_lamina_propria_dendritic_cells_in_a_mouse_model_of_postinfectious_irritable_bowel_syndrome_ DB - PRIME DP - Unbound Medicine ER -