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Expression and localization of the cannabinoid receptor type 1 and the enzyme fatty acid amide hydrolase in the retina of vervet monkeys.
Neuroscience 2012; 202:117-30N

Abstract

The presence of a widespread endocannabinoid (eCB) system within the nervous system, including the retina, has been demonstrated in recent years. Expression patterns of the cannabinoid receptor type 1 (CB1R) and enzyme fatty acid amide hydrolase (FAAH) are available for rodents, but data for humans and monkeys are scarce. We, therefore, thoroughly examined the distribution pattern of CB1R and FAAH throughout the retina of the vervet monkey (Chlorocebus sabeus) using confocal microscopy. Our results demonstrate that CB1R and FAAH are expressed throughout the retina, from the foveal pit to the far periphery. CB1R and FAAH are present in the photoreceptor, outer plexiform, inner nuclear, inner plexiform, and retinal ganglion cell layers (PRL, OPL, INL, IPL, and RGCL, respectively). More specifically, in PRL, CB1R and FAAH are preferentially expressed in cones of the central retina. In OPL, these two components of the eCB system are concentrated not only in the cone pedicles but also in rod spherules with, however, a less intense staining pattern. Triple-labeling immunofluorescence revealed that both cone and rod bipolar cells express CB1R and FAAH. Heavy staining is detected in RGC somas and axons. Neither CB1R nor FAAH are found in the retinal glia, the Müller cells. These data indicate that the eCB system is present throughout the primate retina and is ideally positioned to modulate central and peripheral retinal functions.

Authors+Show Affiliations

School of Optometry, University of Montreal, Montreal, QC, Canada.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22142900

Citation

Bouskila, J, et al. "Expression and Localization of the Cannabinoid Receptor Type 1 and the Enzyme Fatty Acid Amide Hydrolase in the Retina of Vervet Monkeys." Neuroscience, vol. 202, 2012, pp. 117-30.
Bouskila J, Burke MW, Zabouri N, et al. Expression and localization of the cannabinoid receptor type 1 and the enzyme fatty acid amide hydrolase in the retina of vervet monkeys. Neuroscience. 2012;202:117-30.
Bouskila, J., Burke, M. W., Zabouri, N., Casanova, C., Ptito, M., & Bouchard, J. F. (2012). Expression and localization of the cannabinoid receptor type 1 and the enzyme fatty acid amide hydrolase in the retina of vervet monkeys. Neuroscience, 202, pp. 117-30. doi:10.1016/j.neuroscience.2011.11.041.
Bouskila J, et al. Expression and Localization of the Cannabinoid Receptor Type 1 and the Enzyme Fatty Acid Amide Hydrolase in the Retina of Vervet Monkeys. Neuroscience. 2012 Jan 27;202:117-30. PubMed PMID: 22142900.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression and localization of the cannabinoid receptor type 1 and the enzyme fatty acid amide hydrolase in the retina of vervet monkeys. AU - Bouskila,J, AU - Burke,M W, AU - Zabouri,N, AU - Casanova,C, AU - Ptito,M, AU - Bouchard,J-F, Y1 - 2011/11/28/ PY - 2011/09/06/received PY - 2011/10/26/revised PY - 2011/11/17/accepted PY - 2011/12/7/entrez PY - 2011/12/7/pubmed PY - 2012/5/19/medline SP - 117 EP - 30 JF - Neuroscience JO - Neuroscience VL - 202 N2 - The presence of a widespread endocannabinoid (eCB) system within the nervous system, including the retina, has been demonstrated in recent years. Expression patterns of the cannabinoid receptor type 1 (CB1R) and enzyme fatty acid amide hydrolase (FAAH) are available for rodents, but data for humans and monkeys are scarce. We, therefore, thoroughly examined the distribution pattern of CB1R and FAAH throughout the retina of the vervet monkey (Chlorocebus sabeus) using confocal microscopy. Our results demonstrate that CB1R and FAAH are expressed throughout the retina, from the foveal pit to the far periphery. CB1R and FAAH are present in the photoreceptor, outer plexiform, inner nuclear, inner plexiform, and retinal ganglion cell layers (PRL, OPL, INL, IPL, and RGCL, respectively). More specifically, in PRL, CB1R and FAAH are preferentially expressed in cones of the central retina. In OPL, these two components of the eCB system are concentrated not only in the cone pedicles but also in rod spherules with, however, a less intense staining pattern. Triple-labeling immunofluorescence revealed that both cone and rod bipolar cells express CB1R and FAAH. Heavy staining is detected in RGC somas and axons. Neither CB1R nor FAAH are found in the retinal glia, the Müller cells. These data indicate that the eCB system is present throughout the primate retina and is ideally positioned to modulate central and peripheral retinal functions. SN - 1873-7544 UR - https://www.unboundmedicine.com/medline/citation/22142900/Expression_and_localization_of_the_cannabinoid_receptor_type_1_and_the_enzyme_fatty_acid_amide_hydrolase_in_the_retina_of_vervet_monkeys_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0306-4522(11)01326-1 DB - PRIME DP - Unbound Medicine ER -