Tags

Type your tag names separated by a space and hit enter

Antimetastatic potentials of Phyllanthus urinaria L on A549 and Lewis lung carcinoma cells via repression of matrix-degrading proteases.
Integr Cancer Ther. 2012 Sep; 11(3):267-78.IC

Abstract

Tumor metastasis is the most important cause of cancer death and various treatment strategies have targeted at preventing the occurrence of metastasis. Phyllanthus urinaria L is a popular folk medicine and has several proven biological properties, including antioxidant, antihypertension, and anti-inflammatory. This study provides molecular evidence associated with the antimetastatic effects of P urinaria L extracts (PUE), which contained polyphenols including gallic acid, methyl gallate, epicatechin, epigallocatechin-3-gallate, gallocatechin-3-gallate, rutin, epicatechin-3-gallate, and naringin, by showing a marked inhibition on the invasion (P < .001) and migration (P < .001) of highly metastatic A549 and Lewis lung carcinoma (LLC) cells. To further investigate the precise involvement of PUE in tumor metastasis, A549 and LLC cells were treated with PUE at various concentrations and results from zymography and Western blotting showed that a PUE treatment may decrease the expressions of matrix metalloproteinase-2 (MMP-2; P < .001), MMP-9 (P < .001), urokinase plasminogen activator (P < .001), and their endogenous inhibitors, that is, tissue inhibitor of metalloproteinase-2 and plasminogen activator inhibitor-1, in a concentration-dependent manner. Reverse transcription-polymerase chain reaction and MMP-2 promoter luciferase analysis (P < .001) revealed that PUE inhibits the transcription of MMP-2 mRNA. PUE also exerted an inhibitory effect on the DNA-binding activity and the nuclear translocation of NF-κB and AP-1. Furthermore, the inhibitory effects of PUE on the metastasis and growth of LLC cells in vivo were proven. These results indicate that PUE could be applied to be a potential antimetastatic agent.

Authors+Show Affiliations

Antai Tian-Sheng Memorial Hospital, Pingtung, Taiwan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22144737

Citation

Tseng, Hsu-Hung, et al. "Antimetastatic Potentials of Phyllanthus Urinaria L On A549 and Lewis Lung Carcinoma Cells Via Repression of Matrix-degrading Proteases." Integrative Cancer Therapies, vol. 11, no. 3, 2012, pp. 267-78.
Tseng HH, Chen PN, Kuo WH, et al. Antimetastatic potentials of Phyllanthus urinaria L on A549 and Lewis lung carcinoma cells via repression of matrix-degrading proteases. Integr Cancer Ther. 2012;11(3):267-78.
Tseng, H. H., Chen, P. N., Kuo, W. H., Wang, J. W., Chu, S. C., & Hsieh, Y. S. (2012). Antimetastatic potentials of Phyllanthus urinaria L on A549 and Lewis lung carcinoma cells via repression of matrix-degrading proteases. Integrative Cancer Therapies, 11(3), 267-78. https://doi.org/10.1177/1534735411417128
Tseng HH, et al. Antimetastatic Potentials of Phyllanthus Urinaria L On A549 and Lewis Lung Carcinoma Cells Via Repression of Matrix-degrading Proteases. Integr Cancer Ther. 2012;11(3):267-78. PubMed PMID: 22144737.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antimetastatic potentials of Phyllanthus urinaria L on A549 and Lewis lung carcinoma cells via repression of matrix-degrading proteases. AU - Tseng,Hsu-Hung, AU - Chen,Pei-Ni, AU - Kuo,Wu-Hsien, AU - Wang,Jhih-Wei, AU - Chu,Shu-Chen, AU - Hsieh,Yih-Shou, Y1 - 2011/12/05/ PY - 2011/12/7/entrez PY - 2011/12/7/pubmed PY - 2013/2/5/medline SP - 267 EP - 78 JF - Integrative cancer therapies JO - Integr Cancer Ther VL - 11 IS - 3 N2 - Tumor metastasis is the most important cause of cancer death and various treatment strategies have targeted at preventing the occurrence of metastasis. Phyllanthus urinaria L is a popular folk medicine and has several proven biological properties, including antioxidant, antihypertension, and anti-inflammatory. This study provides molecular evidence associated with the antimetastatic effects of P urinaria L extracts (PUE), which contained polyphenols including gallic acid, methyl gallate, epicatechin, epigallocatechin-3-gallate, gallocatechin-3-gallate, rutin, epicatechin-3-gallate, and naringin, by showing a marked inhibition on the invasion (P < .001) and migration (P < .001) of highly metastatic A549 and Lewis lung carcinoma (LLC) cells. To further investigate the precise involvement of PUE in tumor metastasis, A549 and LLC cells were treated with PUE at various concentrations and results from zymography and Western blotting showed that a PUE treatment may decrease the expressions of matrix metalloproteinase-2 (MMP-2; P < .001), MMP-9 (P < .001), urokinase plasminogen activator (P < .001), and their endogenous inhibitors, that is, tissue inhibitor of metalloproteinase-2 and plasminogen activator inhibitor-1, in a concentration-dependent manner. Reverse transcription-polymerase chain reaction and MMP-2 promoter luciferase analysis (P < .001) revealed that PUE inhibits the transcription of MMP-2 mRNA. PUE also exerted an inhibitory effect on the DNA-binding activity and the nuclear translocation of NF-κB and AP-1. Furthermore, the inhibitory effects of PUE on the metastasis and growth of LLC cells in vivo were proven. These results indicate that PUE could be applied to be a potential antimetastatic agent. SN - 1552-695X UR - https://www.unboundmedicine.com/medline/citation/22144737/Antimetastatic_potentials_of_Phyllanthus_urinaria_L_on_A549_and_Lewis_lung_carcinoma_cells_via_repression_of_matrix_degrading_proteases_ L2 - https://journals.sagepub.com/doi/10.1177/1534735411417128?url_ver=Z39.88-2003&amp;rfr_id=ori:rid:crossref.org&amp;rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -