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The evolution of antibody response in armadillos inoculated with Mycobacterium leprae.
Lepr Rev. 1990 Sep; 61(3):215-26.LR

Abstract

Plasma from 30 armadillos (Dasypus novemcinctus) was collected prior to inoculation and at approximately 3-month intervals for a period of 1-3 years. These animals were inoculated intravenously with 6.1 x 10(8) +/- 2 x 10(8) (x +/- SD) armadillo-derived Mycobacterium leprae. These samples were analysed for antibodies of IgM and IgG class to phenolic glycolipid-I (PGL-I) and to sonicated M. leprae components using ELISA and immunoblotting techniques, respectively. We had previously observed among a group of 11 armadillos, that some animals produced and maintained a high IgG antibody response to PGL-I. In this study, an animal's ability to produce and maintain an elevated IgG anti-PGL-I response was significantly correlated with their ability to delay dissemination of the infection and their ability to survive longer. When the animals were moribund, a significant decrease in the IgG anti-PGL-I absorbance value was observed. The detection of PGL-I in the plasma samples collected from moribund armadillos suggested that high concentrations of PGL-I in the plasma may have contributed to a drop in absorbance values by the formation of non-lattice-type immune complexes in vivo. As detected by immunoblotting, the IgM and IgG response to antigens derived from sonically disrupted M. leprae was directed towards molecules with broad bands of immunoreactivity ranging from 21- to 45-kDa. There were no distinguishing features of these antibody responses among armadillos as was evident with the IgG anti-PGL-I responses.

Authors+Show Affiliations

Vadiee AR
Laboratory Research Branch, Gillis W. Long Hansen's Disease Center, Carville, Louisiana 70721.
Harris E
No affiliation info available
Shannon EJ
No affiliation info available

MeSH

AnimalsAntibodies, BacterialArmadillosChromatography, Thin LayerEnzyme-Linked Immunosorbent AssayImmunoblottingImmunoglobulin GImmunoglobulin MMycobacterium leprae

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

2215054

Citation

Vadiee, A R., et al. "The Evolution of Antibody Response in Armadillos Inoculated With Mycobacterium Leprae." Leprosy Review, vol. 61, no. 3, 1990, pp. 215-26.
Vadiee AR, Harris E, Shannon EJ. The evolution of antibody response in armadillos inoculated with Mycobacterium leprae. Lepr Rev. 1990;61(3):215-26.
Vadiee, A. R., Harris, E., & Shannon, E. J. (1990). The evolution of antibody response in armadillos inoculated with Mycobacterium leprae. Leprosy Review, 61(3), 215-26.
Vadiee AR, Harris E, Shannon EJ. The Evolution of Antibody Response in Armadillos Inoculated With Mycobacterium Leprae. Lepr Rev. 1990;61(3):215-26. PubMed PMID: 2215054.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The evolution of antibody response in armadillos inoculated with Mycobacterium leprae. AU - Vadiee,A R, AU - Harris,E, AU - Shannon,E J, PY - 1990/9/1/pubmed PY - 1990/9/1/medline PY - 1990/9/1/entrez SP - 215 EP - 26 JF - Leprosy review JO - Lepr Rev VL - 61 IS - 3 N2 - Plasma from 30 armadillos (Dasypus novemcinctus) was collected prior to inoculation and at approximately 3-month intervals for a period of 1-3 years. These animals were inoculated intravenously with 6.1 x 10(8) +/- 2 x 10(8) (x +/- SD) armadillo-derived Mycobacterium leprae. These samples were analysed for antibodies of IgM and IgG class to phenolic glycolipid-I (PGL-I) and to sonicated M. leprae components using ELISA and immunoblotting techniques, respectively. We had previously observed among a group of 11 armadillos, that some animals produced and maintained a high IgG antibody response to PGL-I. In this study, an animal's ability to produce and maintain an elevated IgG anti-PGL-I response was significantly correlated with their ability to delay dissemination of the infection and their ability to survive longer. When the animals were moribund, a significant decrease in the IgG anti-PGL-I absorbance value was observed. The detection of PGL-I in the plasma samples collected from moribund armadillos suggested that high concentrations of PGL-I in the plasma may have contributed to a drop in absorbance values by the formation of non-lattice-type immune complexes in vivo. As detected by immunoblotting, the IgM and IgG response to antigens derived from sonically disrupted M. leprae was directed towards molecules with broad bands of immunoreactivity ranging from 21- to 45-kDa. There were no distinguishing features of these antibody responses among armadillos as was evident with the IgG anti-PGL-I responses. SN - 0305-7518 UR - https://www.unboundmedicine.com/medline/citation/2215054/The_evolution_of_antibody_response_in_armadillos_inoculated_with_Mycobacterium_leprae_ DB - PRIME DP - Unbound Medicine ER -