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Hydrogen peroxide as a mediator of vasorelaxation evoked by N-oleoylethanolamine and anandamide in rat small mesenteric arteries.
Eur J Pharmacol. 2012 Jan 15; 674(2-3):384-90.EJ

Abstract

Hydrogen peroxide (H(2)O(2)) has been shown to participate in endothelium-derived hyperpolarising factor (EDHF)-mediated mechanisms. Vasorelaxation to the endocannabinoid-like N-oleoylethanolamine (OEA) and anandamide has been shown to be endothelium-dependent. Therefore, the principal aim was to investigate whether H(2)O(2) plays a role in vasorelaxation to endocannabinoids in rat mesenteric arteries. We have also investigated the effects of catalase on endothelium-dependent relaxations and vascular responses to H(2)O(2). First- (G1) and third- (G3) order branches of the superior mesenteric artery from male, Wistar rats were mounted in a wire myograph, contracted with methoxamine, and concentration-response curves to anandamide, OEA carbachol or H(2)O(2), were constructed. The influence of nitric oxide production and H(2)O(2) breakdown on these responses were then investigated using L-NAME (300 μM), and catalase (1000 Uml(-1)) respectively. In G1 mesenteric arteries, vasorelaxations to carbachol and H(2)O(2) were inhibited by L-NAME, but not by catalase. Responses to both anandamide and OEA were also unaffected by catalase. In G3 mesenteric arteries, endothelium-dependent relaxations to carbachol were modestly affected by L-NAME, unaffected by catalase alone, but their combination greatly inhibited vasorelaxation. Similarly, catalase inhibited vasorelaxation to anandamide and OEA, and combined treatment with L-NAME further reduced this response. In G1 mesenteric arteries, vasorelaxation to H(2)O(2) is predominantly mediated by nitric oxide. We conclude that in G3 arteries H(2)O(2) activity contributes towards EDHF-type responses and vasorelaxation to endocannabinoids, either directly or indirectly. Given the association between vascular pathophysiology and H(2)O(2), these findings may provide a mechanism whereby disease states may influence responses to endocannabinoid and related mediators.

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Authors+Show Affiliations

Wheal AJ
Cardiovascular Research Group, School of Biomedical Sciences, University of Nottingham Medical School, Queen's Medical Centre, Nottingham, NG7 2UH, United Kingdom.
Alexander SP
No affiliation info available
Randall MD
No affiliation info available

MeSH

AnimalsArachidonic AcidsCannabinoid Receptor ModulatorsCarbacholCatalaseEndocannabinoidsEthanolaminesHydrogen PeroxideIn Vitro TechniquesMaleMesenteric ArteriesNG-Nitroarginine Methyl EsterOleic AcidsPolyunsaturated AlkamidesRatsRats, WistarVasodilation

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22154756

Citation

Wheal, Amanda J., et al. "Hydrogen Peroxide as a Mediator of Vasorelaxation Evoked By N-oleoylethanolamine and Anandamide in Rat Small Mesenteric Arteries." European Journal of Pharmacology, vol. 674, no. 2-3, 2012, pp. 384-90.
Wheal AJ, Alexander SP, Randall MD. Hydrogen peroxide as a mediator of vasorelaxation evoked by N-oleoylethanolamine and anandamide in rat small mesenteric arteries. Eur J Pharmacol. 2012;674(2-3):384-90.
Wheal, A. J., Alexander, S. P., & Randall, M. D. (2012). Hydrogen peroxide as a mediator of vasorelaxation evoked by N-oleoylethanolamine and anandamide in rat small mesenteric arteries. European Journal of Pharmacology, 674(2-3), 384-90. https://doi.org/10.1016/j.ejphar.2011.11.033
Wheal AJ, Alexander SP, Randall MD. Hydrogen Peroxide as a Mediator of Vasorelaxation Evoked By N-oleoylethanolamine and Anandamide in Rat Small Mesenteric Arteries. Eur J Pharmacol. 2012 Jan 15;674(2-3):384-90. PubMed PMID: 22154756.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hydrogen peroxide as a mediator of vasorelaxation evoked by N-oleoylethanolamine and anandamide in rat small mesenteric arteries. AU - Wheal,Amanda J, AU - Alexander,Stephen P H, AU - Randall,Michael D, Y1 - 2011/11/29/ PY - 2011/07/05/received PY - 2011/11/09/revised PY - 2011/11/16/accepted PY - 2011/12/14/entrez PY - 2011/12/14/pubmed PY - 2012/5/4/medline SP - 384 EP - 90 JF - European journal of pharmacology JO - Eur J Pharmacol VL - 674 IS - 2-3 N2 - Hydrogen peroxide (H(2)O(2)) has been shown to participate in endothelium-derived hyperpolarising factor (EDHF)-mediated mechanisms. Vasorelaxation to the endocannabinoid-like N-oleoylethanolamine (OEA) and anandamide has been shown to be endothelium-dependent. Therefore, the principal aim was to investigate whether H(2)O(2) plays a role in vasorelaxation to endocannabinoids in rat mesenteric arteries. We have also investigated the effects of catalase on endothelium-dependent relaxations and vascular responses to H(2)O(2). First- (G1) and third- (G3) order branches of the superior mesenteric artery from male, Wistar rats were mounted in a wire myograph, contracted with methoxamine, and concentration-response curves to anandamide, OEA carbachol or H(2)O(2), were constructed. The influence of nitric oxide production and H(2)O(2) breakdown on these responses were then investigated using L-NAME (300 μM), and catalase (1000 Uml(-1)) respectively. In G1 mesenteric arteries, vasorelaxations to carbachol and H(2)O(2) were inhibited by L-NAME, but not by catalase. Responses to both anandamide and OEA were also unaffected by catalase. In G3 mesenteric arteries, endothelium-dependent relaxations to carbachol were modestly affected by L-NAME, unaffected by catalase alone, but their combination greatly inhibited vasorelaxation. Similarly, catalase inhibited vasorelaxation to anandamide and OEA, and combined treatment with L-NAME further reduced this response. In G1 mesenteric arteries, vasorelaxation to H(2)O(2) is predominantly mediated by nitric oxide. We conclude that in G3 arteries H(2)O(2) activity contributes towards EDHF-type responses and vasorelaxation to endocannabinoids, either directly or indirectly. Given the association between vascular pathophysiology and H(2)O(2), these findings may provide a mechanism whereby disease states may influence responses to endocannabinoid and related mediators. SN - 1879-0712 UR - https://www.unboundmedicine.com/medline/citation/22154756/Hydrogen_peroxide_as_a_mediator_of_vasorelaxation_evoked_by_N_oleoylethanolamine_and_anandamide_in_rat_small_mesenteric_arteries_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-2999(11)01500-7 DB - PRIME DP - Unbound Medicine ER -