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Ultrasensitive electrochemiluminescence immunoassay for tumor marker detection using functionalized Ru-silica@nanoporous gold composite as labels.
Analyst 2012; 137(3):680-5A

Abstract

In this work, we reported a simple and sensitive sandwich-type electrochemiluminescence (ECL) immunosensor for carcinoembryonic antigen (CEA) on a gold nanoparticles (AuNPs) modified glassy carbon electrode (GCE). The Ru-silica (Ru(bpy)(3)(2+)-doped silica) capped nanoporous gold (NPG) (Ru-silica@NPG) composite was used as an excellent label with amplification techniques. The NPG was prepared with a simple dealloying strategy, by which silver was dissolved from silver/gold alloys in nitric acid. The primary antibody was immobilized on the AuNPs modified electrode through l-cysteine and glutaraldehyde, and then the antigen and the functionalized Ru-silica@NPG composite labeled secondary antibody were conjugated successively to form a sandwich-type immunocomplex through the specific interaction. The concentrations of CEA were obtained in the range from 1 pg mL(-1) to 10 ng mL(-1) with a detection limit of 0.8 pg mL(-1). The as-proposed ECL immunosensor has the advantages of high sensitivity, specificity and stability and could become a promising technique for tumor marker detection.

Authors+Show Affiliations

Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, University of Jinan, Jinan, 250022, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22158639

Citation

Zhang, Meng, et al. "Ultrasensitive Electrochemiluminescence Immunoassay for Tumor Marker Detection Using Functionalized Ru-silica@nanoporous Gold Composite as Labels." The Analyst, vol. 137, no. 3, 2012, pp. 680-5.
Zhang M, Ge S, Li W, et al. Ultrasensitive electrochemiluminescence immunoassay for tumor marker detection using functionalized Ru-silica@nanoporous gold composite as labels. Analyst. 2012;137(3):680-5.
Zhang, M., Ge, S., Li, W., Yan, M., Song, X., Yu, J., ... Huang, J. (2012). Ultrasensitive electrochemiluminescence immunoassay for tumor marker detection using functionalized Ru-silica@nanoporous gold composite as labels. The Analyst, 137(3), pp. 680-5. doi:10.1039/c1an15942a.
Zhang M, et al. Ultrasensitive Electrochemiluminescence Immunoassay for Tumor Marker Detection Using Functionalized Ru-silica@nanoporous Gold Composite as Labels. Analyst. 2012 Feb 7;137(3):680-5. PubMed PMID: 22158639.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ultrasensitive electrochemiluminescence immunoassay for tumor marker detection using functionalized Ru-silica@nanoporous gold composite as labels. AU - Zhang,Meng, AU - Ge,Shenguang, AU - Li,Weiping, AU - Yan,Mei, AU - Song,Xianrang, AU - Yu,Jinghua, AU - Xu,Wei, AU - Huang,Jiadong, Y1 - 2011/12/07/ PY - 2011/12/14/entrez PY - 2011/12/14/pubmed PY - 2012/5/2/medline SP - 680 EP - 5 JF - The Analyst JO - Analyst VL - 137 IS - 3 N2 - In this work, we reported a simple and sensitive sandwich-type electrochemiluminescence (ECL) immunosensor for carcinoembryonic antigen (CEA) on a gold nanoparticles (AuNPs) modified glassy carbon electrode (GCE). The Ru-silica (Ru(bpy)(3)(2+)-doped silica) capped nanoporous gold (NPG) (Ru-silica@NPG) composite was used as an excellent label with amplification techniques. The NPG was prepared with a simple dealloying strategy, by which silver was dissolved from silver/gold alloys in nitric acid. The primary antibody was immobilized on the AuNPs modified electrode through l-cysteine and glutaraldehyde, and then the antigen and the functionalized Ru-silica@NPG composite labeled secondary antibody were conjugated successively to form a sandwich-type immunocomplex through the specific interaction. The concentrations of CEA were obtained in the range from 1 pg mL(-1) to 10 ng mL(-1) with a detection limit of 0.8 pg mL(-1). The as-proposed ECL immunosensor has the advantages of high sensitivity, specificity and stability and could become a promising technique for tumor marker detection. SN - 1364-5528 UR - https://www.unboundmedicine.com/medline/citation/22158639/Ultrasensitive_electrochemiluminescence_immunoassay_for_tumor_marker_detection_using_functionalized_Ru_silica@nanoporous_gold_composite_as_labels_ L2 - https://doi.org/10.1039/c1an15942a DB - PRIME DP - Unbound Medicine ER -