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Effect of dietary polyunsaturated fatty acids on castration-resistant Pten-null prostate cancer.
Carcinogenesis. 2012 Feb; 33(2):404-12.C

Abstract

A common treatment of advanced prostate cancer involves the deprivation of androgens. Despite the initial response to hormonal therapy, eventually all the patients relapse. In the present study, we sought to determine whether dietary polyunsaturated fatty acid (PUFA) affects the development of castration-resistant prostate cancer. Cell culture, patient tissue microarray, allograft, xenograft, prostate-specific Pten knockout and omega-3 desaturase transgenic mouse models in conjunction with dietary manipulation, gene knockdown and knockout approaches were used to determine the effect of dietary PUFA on castration-resistant Pten-null prostate cancer. We found that deletion of Pten increased androgen receptor (AR) expression and Pten-null prostate cells were castration resistant. Omega-3 PUFA slowed down the growth of castration-resistant tumors as compared with omega-6 PUFA. Omega-3 PUFA decreased AR protein to a similar extent in tumor cell cytosolic and nuclear fractions but had no effect on AR messenger RNA level. Omega-3 PUFA treatment appeared to accelerate AR protein degradation, which could be blocked by proteasome inhibitor MG132. Knockdown of AR significantly slowed down prostate cancer cell proliferation in the absence of androgens. Our data suggest that omega-3 PUFA inhibits castration-resistant prostate cancer in part by accelerating proteasome-dependent degradation of the AR protein. Dietary omega-3 PUFA supplementation in conjunction with androgen ablation may significantly delay the development of castration-resistant prostate cancer in patients compared with androgen ablation alone.

Authors+Show Affiliations

Department of Cancer Biology, Wake Forest University School of Medicine, Medical Center Blvd., Winston-Salem, NC 27157, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22159221

Citation

Wang, Shihua, et al. "Effect of Dietary Polyunsaturated Fatty Acids On Castration-resistant Pten-null Prostate Cancer." Carcinogenesis, vol. 33, no. 2, 2012, pp. 404-12.
Wang S, Wu J, Suburu J, et al. Effect of dietary polyunsaturated fatty acids on castration-resistant Pten-null prostate cancer. Carcinogenesis. 2012;33(2):404-12.
Wang, S., Wu, J., Suburu, J., Gu, Z., Cai, J., Axanova, L. S., Cramer, S. D., Thomas, M. J., Perry, D. L., Edwards, I. J., Mucci, L. A., Sinnott, J. A., Loda, M. F., Sui, G., Berquin, I. M., & Chen, Y. Q. (2012). Effect of dietary polyunsaturated fatty acids on castration-resistant Pten-null prostate cancer. Carcinogenesis, 33(2), 404-12. https://doi.org/10.1093/carcin/bgr290
Wang S, et al. Effect of Dietary Polyunsaturated Fatty Acids On Castration-resistant Pten-null Prostate Cancer. Carcinogenesis. 2012;33(2):404-12. PubMed PMID: 22159221.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of dietary polyunsaturated fatty acids on castration-resistant Pten-null prostate cancer. AU - Wang,Shihua, AU - Wu,Jiansheng, AU - Suburu,Janel, AU - Gu,Zhennan, AU - Cai,Jiaozhong, AU - Axanova,Linara S, AU - Cramer,Scott D, AU - Thomas,Michael J, AU - Perry,Donna L, AU - Edwards,Iris J, AU - Mucci,Lorelei A, AU - Sinnott,Jennifer A, AU - Loda,Massimo F, AU - Sui,Guangchao, AU - Berquin,Isabelle M, AU - Chen,Yong Q, Y1 - 2011/12/08/ PY - 2011/12/14/entrez PY - 2011/12/14/pubmed PY - 2012/6/13/medline SP - 404 EP - 12 JF - Carcinogenesis JO - Carcinogenesis VL - 33 IS - 2 N2 - A common treatment of advanced prostate cancer involves the deprivation of androgens. Despite the initial response to hormonal therapy, eventually all the patients relapse. In the present study, we sought to determine whether dietary polyunsaturated fatty acid (PUFA) affects the development of castration-resistant prostate cancer. Cell culture, patient tissue microarray, allograft, xenograft, prostate-specific Pten knockout and omega-3 desaturase transgenic mouse models in conjunction with dietary manipulation, gene knockdown and knockout approaches were used to determine the effect of dietary PUFA on castration-resistant Pten-null prostate cancer. We found that deletion of Pten increased androgen receptor (AR) expression and Pten-null prostate cells were castration resistant. Omega-3 PUFA slowed down the growth of castration-resistant tumors as compared with omega-6 PUFA. Omega-3 PUFA decreased AR protein to a similar extent in tumor cell cytosolic and nuclear fractions but had no effect on AR messenger RNA level. Omega-3 PUFA treatment appeared to accelerate AR protein degradation, which could be blocked by proteasome inhibitor MG132. Knockdown of AR significantly slowed down prostate cancer cell proliferation in the absence of androgens. Our data suggest that omega-3 PUFA inhibits castration-resistant prostate cancer in part by accelerating proteasome-dependent degradation of the AR protein. Dietary omega-3 PUFA supplementation in conjunction with androgen ablation may significantly delay the development of castration-resistant prostate cancer in patients compared with androgen ablation alone. SN - 1460-2180 UR - https://www.unboundmedicine.com/medline/citation/22159221/Effect_of_dietary_polyunsaturated_fatty_acids_on_castration_resistant_Pten_null_prostate_cancer_ L2 - https://academic.oup.com/carcin/article-lookup/doi/10.1093/carcin/bgr290 DB - PRIME DP - Unbound Medicine ER -