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Bacterial assisted phytoremediation for enhanced degradation of highly sulfonated diazo reactive dye.
Environ Sci Pollut Res Int. 2012 Jun; 19(5):1709-18.ES

Abstract

PURPOSE

Phytoremediation is the exploitation of plants and their rhizospheric microorganisms for pollutants treatment like textile dyes, which are toxic, carcinogenic and mutagenic from the effluent. The purpose of this work was to explore a naturally found plant and bacterial synergism to achieve an enhanced degradation of Remazol Black B dye (RBB).

METHODS

In vitro cultures of Zinnia angustifolia were obtained by seed culture method. Enzymatic analysis of the plant roots and Exiguobacterium aestuarii strain ZaK cells was performed before and after decolorization of RBB. Metabolites of RBB formed after its degradation were analyzed using UV-Vis spectroscopy, high-performance liquid chromatography (HPLC), Fourier transform infrared (FTIR) and gas chromatography-mass spectrometry (GC-MS). Phytotoxicity studies were performed.

RESULTS

The consortium ZE was found to be more efficient than individual plant and bacteria. Z. angustifolia roots showed significant induction in the activities of lignin peroxidase, laccase, DCIP reductase and tyrosinase during dye decolorization. E. aestuarii showed significant induction in the activities of veratryl alcohol oxidase, azo reductase and DCIP reductase. Analysis of metabolites revealed differential metabolism of RBB by plant, bacteria and consortium ZE. E. aestuarii and Z. angustifolia led to the formation of 3,6-diamino-4-hydroxynaphthalene-2-sulfonic acid, (ethylsulfonyl)benzene, and 3,4,6-trihydroxynaphthalene-2-sulfonic acid and propane-1-sulfonic acid, respectively, whereas consortium ZE produced 4-hydroxynaphthalene-2-sulfonic acid, naphthalene-2-sulfonic acid and 4-(methylsulfonyl)phenol. The phytotoxicity study revealed the nontoxic nature of the metabolites formed after dye degradation.

CONCLUSION

Consortium ZE was found to be more efficient and faster in the degradation of RBB when compared to degradation by Z. angustifoila and E. aestuarii individually.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Khandare RV
Department of Biotechnology, Shivaji University, Kolhapur, India.
Rane NR
No affiliation info available
Waghmode TR
No affiliation info available
Govindwar SP
No affiliation info available

MeSH

AsteraceaeBacillalesBiodegradation, EnvironmentalChromatography, High Pressure LiquidEnzymesGas Chromatography-Mass SpectrometryGerminationLaccaseNaphthalenesulfonatesPhylogenySpectroscopy, Fourier Transform InfraredSymbiosisTextile IndustryToxicity TestsWater Pollutants, Chemical

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22161298

Citation

Khandare, Rahul V., et al. "Bacterial Assisted Phytoremediation for Enhanced Degradation of Highly Sulfonated Diazo Reactive Dye." Environmental Science and Pollution Research International, vol. 19, no. 5, 2012, pp. 1709-18.
Khandare RV, Rane NR, Waghmode TR, et al. Bacterial assisted phytoremediation for enhanced degradation of highly sulfonated diazo reactive dye. Environ Sci Pollut Res Int. 2012;19(5):1709-18.
Khandare, R. V., Rane, N. R., Waghmode, T. R., & Govindwar, S. P. (2012). Bacterial assisted phytoremediation for enhanced degradation of highly sulfonated diazo reactive dye. Environmental Science and Pollution Research International, 19(5), 1709-18. https://doi.org/10.1007/s11356-011-0679-x
Khandare RV, et al. Bacterial Assisted Phytoremediation for Enhanced Degradation of Highly Sulfonated Diazo Reactive Dye. Environ Sci Pollut Res Int. 2012;19(5):1709-18. PubMed PMID: 22161298.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Bacterial assisted phytoremediation for enhanced degradation of highly sulfonated diazo reactive dye. AU - Khandare,Rahul V, AU - Rane,Niraj R, AU - Waghmode,Tatoba R, AU - Govindwar,Sanjay P, Y1 - 2011/12/09/ PY - 2011/08/10/received PY - 2011/11/22/accepted PY - 2011/12/14/entrez PY - 2011/12/14/pubmed PY - 2012/9/27/medline SP - 1709 EP - 18 JF - Environmental science and pollution research international JO - Environ Sci Pollut Res Int VL - 19 IS - 5 N2 - PURPOSE: Phytoremediation is the exploitation of plants and their rhizospheric microorganisms for pollutants treatment like textile dyes, which are toxic, carcinogenic and mutagenic from the effluent. The purpose of this work was to explore a naturally found plant and bacterial synergism to achieve an enhanced degradation of Remazol Black B dye (RBB). METHODS: In vitro cultures of Zinnia angustifolia were obtained by seed culture method. Enzymatic analysis of the plant roots and Exiguobacterium aestuarii strain ZaK cells was performed before and after decolorization of RBB. Metabolites of RBB formed after its degradation were analyzed using UV-Vis spectroscopy, high-performance liquid chromatography (HPLC), Fourier transform infrared (FTIR) and gas chromatography-mass spectrometry (GC-MS). Phytotoxicity studies were performed. RESULTS: The consortium ZE was found to be more efficient than individual plant and bacteria. Z. angustifolia roots showed significant induction in the activities of lignin peroxidase, laccase, DCIP reductase and tyrosinase during dye decolorization. E. aestuarii showed significant induction in the activities of veratryl alcohol oxidase, azo reductase and DCIP reductase. Analysis of metabolites revealed differential metabolism of RBB by plant, bacteria and consortium ZE. E. aestuarii and Z. angustifolia led to the formation of 3,6-diamino-4-hydroxynaphthalene-2-sulfonic acid, (ethylsulfonyl)benzene, and 3,4,6-trihydroxynaphthalene-2-sulfonic acid and propane-1-sulfonic acid, respectively, whereas consortium ZE produced 4-hydroxynaphthalene-2-sulfonic acid, naphthalene-2-sulfonic acid and 4-(methylsulfonyl)phenol. The phytotoxicity study revealed the nontoxic nature of the metabolites formed after dye degradation. CONCLUSION: Consortium ZE was found to be more efficient and faster in the degradation of RBB when compared to degradation by Z. angustifoila and E. aestuarii individually. SN - 1614-7499 UR - https://www.unboundmedicine.com/medline/citation/22161298/Bacterial_assisted_phytoremediation_for_enhanced_degradation_of_highly_sulfonated_diazo_reactive_dye_ L2 - https://dx.doi.org/10.1007/s11356-011-0679-x DB - PRIME DP - Unbound Medicine ER -