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Comparison of dacron and nylon-flocked self-collected vaginal swabs and urine for the detection of Trichomonas vaginalis using analyte-specific reagents in a transcription-mediated amplification assay.
Sex Transm Infect. 2012 Apr; 88(3):160-2.ST

Abstract

OBJECTIVES

To compare self-collected vaginal swab (SCVS) types and first-catch urine (FCU) to diagnose Trichomonas vaginalis using analyte-specific reagents designed to be used in a transcription-mediated amplification assay.

METHODS

A total of 241 women (group A) collected a FCU and a SCVS using a dacron swab (APTIMA collection kit). A second group of 289 women (group B) collected two SCVS using one dacron swab and one nylon-flocked swab.

RESULTS

Of 75 young women (street youth) determined to be infected with T vaginalis only seven reported symptoms of vaginal discharge or irritation. Using a cutoff of 50,000 relative light units, the sensitivity and specificity was 97.2% and 97.6%, respectively for dacron SCVS compared with 41.7% and 100% for FCU in group A; 92.3% and 98.8% for dacron SCVS and 92.3% and 99.2% for flocked-nylon SCVS in group B. The assay tested 96 samples in 6 h.

CONCLUSIONS

Dacron and nylon-flocked SCVS performed equally well and significantly better than FCU using analyte-specific reagents in the APTIMA transcription-mediated amplification assay. Either swab type could be used for self-collection.

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Authors+Show Affiliations

Jang D
St. Joseph's Healthcare, 50 Charlton Avenue East, Hamilton, ON L8N 4A6, Canada.
Gilchrist J
No affiliation info available
Portillo E
No affiliation info available
Smieja M
No affiliation info available
Toor R
No affiliation info available
Chernesky M
No affiliation info available

MeSH

DNA, ProtozoanFemaleHumansMolecular Diagnostic TechniquesNucleic Acid Amplification TechniquesNylonsParasitologyPolyethylene TerephthalatesSelf-ExaminationSensitivity and SpecificitySpecimen HandlingTrichomonas VaginitisTrichomonas vaginalisUrineVaginaYoung Adult

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22166474

Citation

Jang, Dan, et al. "Comparison of Dacron and Nylon-flocked Self-collected Vaginal Swabs and Urine for the Detection of Trichomonas Vaginalis Using Analyte-specific Reagents in a Transcription-mediated Amplification Assay." Sexually Transmitted Infections, vol. 88, no. 3, 2012, pp. 160-2.
Jang D, Gilchrist J, Portillo E, et al. Comparison of dacron and nylon-flocked self-collected vaginal swabs and urine for the detection of Trichomonas vaginalis using analyte-specific reagents in a transcription-mediated amplification assay. Sex Transm Infect. 2012;88(3):160-2.
Jang, D., Gilchrist, J., Portillo, E., Smieja, M., Toor, R., & Chernesky, M. (2012). Comparison of dacron and nylon-flocked self-collected vaginal swabs and urine for the detection of Trichomonas vaginalis using analyte-specific reagents in a transcription-mediated amplification assay. Sexually Transmitted Infections, 88(3), 160-2. https://doi.org/10.1136/sextrans-2011-050084
Jang D, et al. Comparison of Dacron and Nylon-flocked Self-collected Vaginal Swabs and Urine for the Detection of Trichomonas Vaginalis Using Analyte-specific Reagents in a Transcription-mediated Amplification Assay. Sex Transm Infect. 2012;88(3):160-2. PubMed PMID: 22166474.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of dacron and nylon-flocked self-collected vaginal swabs and urine for the detection of Trichomonas vaginalis using analyte-specific reagents in a transcription-mediated amplification assay. AU - Jang,Dan, AU - Gilchrist,Jodi, AU - Portillo,Eder, AU - Smieja,Marek, AU - Toor,Ramandeep, AU - Chernesky,Max, Y1 - 2011/12/13/ PY - 2011/12/15/entrez PY - 2011/12/15/pubmed PY - 2012/5/4/medline SP - 160 EP - 2 JF - Sexually transmitted infections JO - Sex Transm Infect VL - 88 IS - 3 N2 - OBJECTIVES: To compare self-collected vaginal swab (SCVS) types and first-catch urine (FCU) to diagnose Trichomonas vaginalis using analyte-specific reagents designed to be used in a transcription-mediated amplification assay. METHODS: A total of 241 women (group A) collected a FCU and a SCVS using a dacron swab (APTIMA collection kit). A second group of 289 women (group B) collected two SCVS using one dacron swab and one nylon-flocked swab. RESULTS: Of 75 young women (street youth) determined to be infected with T vaginalis only seven reported symptoms of vaginal discharge or irritation. Using a cutoff of 50,000 relative light units, the sensitivity and specificity was 97.2% and 97.6%, respectively for dacron SCVS compared with 41.7% and 100% for FCU in group A; 92.3% and 98.8% for dacron SCVS and 92.3% and 99.2% for flocked-nylon SCVS in group B. The assay tested 96 samples in 6 h. CONCLUSIONS: Dacron and nylon-flocked SCVS performed equally well and significantly better than FCU using analyte-specific reagents in the APTIMA transcription-mediated amplification assay. Either swab type could be used for self-collection. SN - 1472-3263 UR - https://www.unboundmedicine.com/medline/citation/22166474/Comparison_of_dacron_and_nylon_flocked_self_collected_vaginal_swabs_and_urine_for_the_detection_of_Trichomonas_vaginalis_using_analyte_specific_reagents_in_a_transcription_mediated_amplification_assay_ L2 - https://sti.bmj.com/lookup/pmidlookup?view=long&pmid=22166474 DB - PRIME DP - Unbound Medicine ER -