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The effect of peroral administration of toxic cyanobacteria on laboratory rats (Rattus norvegicus var. alba).
Neuro Endocrinol Lett. 2011; 32 Suppl 1:35-45.NE

Abstract

OBJECTIVES

The toxic cyanobacteria are a serious problem for water supply systems, recreation, and agriculture. Cyanobacteria produce numerous bioactive compounds including microcystins - the most studied cyanobacterial hepatotoxins. Only rare studies addressed realistic situation, i.e. impact of MCs accumulated in the fish tissues on the overall physiology. The aim of the present study was to provide a model simulation of the simple food chain for evaluation of impacts of cyanobacteria on the rat physiology under different exposure scenario.

METHODS

Experimental rats were fed with food with fish meat, which contained external additions of isolated microcystins as well as toxic cyanobacteria Microcystis, nontoxic cyanobacteria Arthrospira and green alga Chlorella. Subgroups of the animals were also challenged with a model antigen KLH to investigated immune-related parameters. We studied parameters of oxidative stress in the liver as levels of lipid peroxidation and glutathion levels. Series of hematological, biochemical and immunological parameters were also investigated.

RESULTS

Although considerable amounts of microcystins were administered to rats, all levels of MCs were under the detection limit (1 ng/g fresh weight) in the rat tissues using tandem LC/MS. Only some conjugates of microcystins with cystein and glutathion were detected in the rat liver exposed to Microcystis biomass (values were around the detection limit). Statistically significant depletion of body and liver weight was observed in groups with microcystin addition in comparison with all other groups. Rats exposed to MCs had stimulated immune system (showed higher antibody answer on administered antigen). Also modulation of some lymphocyte subpopulations was recorded with the most interesting observation of stimulated NK cell numbers in groups exposed to isolated toxins (but not to biomass containing the same toxin amount).

CONCLUSIONS

Our study demonstrates that oral exposure to microcystins in the diet may induce some detoxification responses and modulation of some hematological and immunological parameters.

Authors+Show Affiliations

Research Centre for Toxic Compounds in the Environment (RECETOX), Masaryk University - Faculty of Science Brno, Czech Republic.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22167221

Citation

Adamovsky, Ondrej, et al. "The Effect of Peroral Administration of Toxic Cyanobacteria On Laboratory Rats (Rattus Norvegicus Var. Alba)." Neuro Endocrinology Letters, vol. 32 Suppl 1, 2011, pp. 35-45.
Adamovsky O, Kopp R, Zikova A, et al. The effect of peroral administration of toxic cyanobacteria on laboratory rats (Rattus norvegicus var. alba). Neuro Endocrinol Lett. 2011;32 Suppl 1:35-45.
Adamovsky, O., Kopp, R., Zikova, A., Blaha, L., Kohoutek, J., Ondrackova, P., Paskerova, H., Mares, J., & Palikova, M. (2011). The effect of peroral administration of toxic cyanobacteria on laboratory rats (Rattus norvegicus var. alba). Neuro Endocrinology Letters, 32 Suppl 1, 35-45.
Adamovsky O, et al. The Effect of Peroral Administration of Toxic Cyanobacteria On Laboratory Rats (Rattus Norvegicus Var. Alba). Neuro Endocrinol Lett. 2011;32 Suppl 1:35-45. PubMed PMID: 22167221.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The effect of peroral administration of toxic cyanobacteria on laboratory rats (Rattus norvegicus var. alba). AU - Adamovsky,Ondrej, AU - Kopp,Radovan, AU - Zikova,Andrea, AU - Blaha,Ludek, AU - Kohoutek,Jiri, AU - Ondrackova,Petra, AU - Paskerova,Hana, AU - Mares,Jan, AU - Palikova,Miloslava, PY - 2011/05/20/received PY - 2011/08/25/accepted PY - 2011/12/15/entrez PY - 2011/12/15/pubmed PY - 2012/4/13/medline SP - 35 EP - 45 JF - Neuro endocrinology letters JO - Neuro Endocrinol. Lett. VL - 32 Suppl 1 N2 - OBJECTIVES: The toxic cyanobacteria are a serious problem for water supply systems, recreation, and agriculture. Cyanobacteria produce numerous bioactive compounds including microcystins - the most studied cyanobacterial hepatotoxins. Only rare studies addressed realistic situation, i.e. impact of MCs accumulated in the fish tissues on the overall physiology. The aim of the present study was to provide a model simulation of the simple food chain for evaluation of impacts of cyanobacteria on the rat physiology under different exposure scenario. METHODS: Experimental rats were fed with food with fish meat, which contained external additions of isolated microcystins as well as toxic cyanobacteria Microcystis, nontoxic cyanobacteria Arthrospira and green alga Chlorella. Subgroups of the animals were also challenged with a model antigen KLH to investigated immune-related parameters. We studied parameters of oxidative stress in the liver as levels of lipid peroxidation and glutathion levels. Series of hematological, biochemical and immunological parameters were also investigated. RESULTS: Although considerable amounts of microcystins were administered to rats, all levels of MCs were under the detection limit (1 ng/g fresh weight) in the rat tissues using tandem LC/MS. Only some conjugates of microcystins with cystein and glutathion were detected in the rat liver exposed to Microcystis biomass (values were around the detection limit). Statistically significant depletion of body and liver weight was observed in groups with microcystin addition in comparison with all other groups. Rats exposed to MCs had stimulated immune system (showed higher antibody answer on administered antigen). Also modulation of some lymphocyte subpopulations was recorded with the most interesting observation of stimulated NK cell numbers in groups exposed to isolated toxins (but not to biomass containing the same toxin amount). CONCLUSIONS: Our study demonstrates that oral exposure to microcystins in the diet may induce some detoxification responses and modulation of some hematological and immunological parameters. SN - 0172-780X UR - https://www.unboundmedicine.com/medline/citation/22167221/The_effect_of_peroral_administration_of_toxic_cyanobacteria_on_laboratory_rats__Rattus_norvegicus_var__alba__ DB - PRIME DP - Unbound Medicine ER -