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A sulfated galactan with antioxidant capacity from the green variant of tetrasporic Gigartina skottsbergii (Gigartinales, Rhodophyta).
Carbohydr Res. 2012 Jan 10; 347(1):114-20.CR

Abstract

The water soluble polysaccharide produced by the green variant of tetrasporic Gigartina skottsbergii was found to be composed of D-galactose and sulfate groups in a molar ratio of 1.0:0.65. (1)H and (13)C NMR spectroscopy studies of the desulfated polysaccharide showed a major backbone structure of alternating 3-linked β-D-galactopyranosyl and 4-linked α-D-galactopyranosyl units, and minor signals ascribed to 3-O-methyl-substitution on the latter unit. Ethylation analysis of the polysaccharide indicated that the sulfate groups are mainly located at position O-2 of 4-linked α-D-galactopyranosyl residue and partially located at positions O-6 of the same unit and at position O-2 of 3-linked β-D-galactopyranosyl residue, and confirmed the presence of 3-O-methyl-galactose in minor amounts (4.4%). The sulfated d-galactan presents a similar structure to λ carrageenan but with much lower sulfation at position O-6 of the α-residue and at position O-2 of β-residue. The antioxidant capacity of the sulfated d-galactan was evaluated by the peroxyl radicals (ORAC method), hydroxyl radicals, chelating activity, and ABTS(+) assays. Kinetic results obtained in these assays were compared with those obtained for the commercial λ carrageenan. The antioxidant activity toward peroxyl radicals was higher for commercial λ carrageenan, this agrees with its higher content of sulfate group. The kinetics of the reaction of both polysaccharides with hydroxyl and ABTS(+) radicals showed a complex mechanism, but the antioxidant activity was higher for the polysaccharide from the green variant of tetrasporic Gigartina skottsbergii.

Authors+Show Affiliations

Departamento de Ciencias del Ambiente, Facultad de Química y Biología, Universidad de Santiago de Chile, Av. B. O'Higgins 3363, Santiago, Chile.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22169178

Citation

Barahona, Tamara, et al. "A Sulfated Galactan With Antioxidant Capacity From the Green Variant of Tetrasporic Gigartina Skottsbergii (Gigartinales, Rhodophyta)." Carbohydrate Research, vol. 347, no. 1, 2012, pp. 114-20.
Barahona T, Encinas MV, Mansilla A, et al. A sulfated galactan with antioxidant capacity from the green variant of tetrasporic Gigartina skottsbergii (Gigartinales, Rhodophyta). Carbohydr Res. 2012;347(1):114-20.
Barahona, T., Encinas, M. V., Mansilla, A., Matsuhiro, B., & Zúñiga, E. A. (2012). A sulfated galactan with antioxidant capacity from the green variant of tetrasporic Gigartina skottsbergii (Gigartinales, Rhodophyta). Carbohydrate Research, 347(1), 114-20. https://doi.org/10.1016/j.carres.2011.11.014
Barahona T, et al. A Sulfated Galactan With Antioxidant Capacity From the Green Variant of Tetrasporic Gigartina Skottsbergii (Gigartinales, Rhodophyta). Carbohydr Res. 2012 Jan 10;347(1):114-20. PubMed PMID: 22169178.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A sulfated galactan with antioxidant capacity from the green variant of tetrasporic Gigartina skottsbergii (Gigartinales, Rhodophyta). AU - Barahona,Tamara, AU - Encinas,María V, AU - Mansilla,Andrés, AU - Matsuhiro,Betty, AU - Zúñiga,Elisa A, Y1 - 2011/11/19/ PY - 2011/10/06/received PY - 2011/11/09/revised PY - 2011/11/12/accepted PY - 2011/12/16/entrez PY - 2011/12/16/pubmed PY - 2012/5/5/medline SP - 114 EP - 20 JF - Carbohydrate research JO - Carbohydr Res VL - 347 IS - 1 N2 - The water soluble polysaccharide produced by the green variant of tetrasporic Gigartina skottsbergii was found to be composed of D-galactose and sulfate groups in a molar ratio of 1.0:0.65. (1)H and (13)C NMR spectroscopy studies of the desulfated polysaccharide showed a major backbone structure of alternating 3-linked β-D-galactopyranosyl and 4-linked α-D-galactopyranosyl units, and minor signals ascribed to 3-O-methyl-substitution on the latter unit. Ethylation analysis of the polysaccharide indicated that the sulfate groups are mainly located at position O-2 of 4-linked α-D-galactopyranosyl residue and partially located at positions O-6 of the same unit and at position O-2 of 3-linked β-D-galactopyranosyl residue, and confirmed the presence of 3-O-methyl-galactose in minor amounts (4.4%). The sulfated d-galactan presents a similar structure to λ carrageenan but with much lower sulfation at position O-6 of the α-residue and at position O-2 of β-residue. The antioxidant capacity of the sulfated d-galactan was evaluated by the peroxyl radicals (ORAC method), hydroxyl radicals, chelating activity, and ABTS(+) assays. Kinetic results obtained in these assays were compared with those obtained for the commercial λ carrageenan. The antioxidant activity toward peroxyl radicals was higher for commercial λ carrageenan, this agrees with its higher content of sulfate group. The kinetics of the reaction of both polysaccharides with hydroxyl and ABTS(+) radicals showed a complex mechanism, but the antioxidant activity was higher for the polysaccharide from the green variant of tetrasporic Gigartina skottsbergii. SN - 1873-426X UR - https://www.unboundmedicine.com/medline/citation/22169178/A_sulfated_galactan_with_antioxidant_capacity_from_the_green_variant_of_tetrasporic_Gigartina_skottsbergii__Gigartinales_Rhodophyta__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0008-6215(11)00561-1 DB - PRIME DP - Unbound Medicine ER -