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Targeting of the RhoGEF Ect2 to the equatorial membrane controls cleavage furrow formation during cytokinesis.
Dev Cell 2011; 21(6):1104-15DC

Abstract

In animal cells, formation of the cytokinetic furrow requires activation of the GTPase RhoA by the guanine nucleotide exchange factor Ect2. How Ect2, which is associated with the spindle midzone, controls RhoA activity at the equatorial cortex during anaphase is not understood. Here, we show that Ect2 concentrates at the equatorial membrane during cytokinesis in live cells. Ect2 membrane association requires a pleckstrin homology domain and a polybasic cluster that bind to phosphoinositide lipids. Both guanine nucleotide exchange function and membrane targeting of Ect2 are essential for RhoA activation and cleavage furrow formation in human cells. Membrane localization of Ect2 is spatially confined to the equator by centralspindlin, Ect2's spindle midzone anchor complex, and is temporally coordinated with chromosome segregation through the activation state of CDK1. We propose that targeting of Ect2 to the equatorial membrane represents a key step in the delivery of the cytokinetic signal to the cortex.

Authors+Show Affiliations

Cell Division and Aneuploidy Laboratory, Cancer Research UK London Research Institute, Clare Hall Laboratories, Blanche Lane, South Mimms, Hertfordshire EN6 3LD, UK.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22172673

Citation

Su, Kuan-Chung, et al. "Targeting of the RhoGEF Ect2 to the Equatorial Membrane Controls Cleavage Furrow Formation During Cytokinesis." Developmental Cell, vol. 21, no. 6, 2011, pp. 1104-15.
Su KC, Takaki T, Petronczki M. Targeting of the RhoGEF Ect2 to the equatorial membrane controls cleavage furrow formation during cytokinesis. Dev Cell. 2011;21(6):1104-15.
Su, K. C., Takaki, T., & Petronczki, M. (2011). Targeting of the RhoGEF Ect2 to the equatorial membrane controls cleavage furrow formation during cytokinesis. Developmental Cell, 21(6), pp. 1104-15. doi:10.1016/j.devcel.2011.11.003.
Su KC, Takaki T, Petronczki M. Targeting of the RhoGEF Ect2 to the Equatorial Membrane Controls Cleavage Furrow Formation During Cytokinesis. Dev Cell. 2011 Dec 13;21(6):1104-15. PubMed PMID: 22172673.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Targeting of the RhoGEF Ect2 to the equatorial membrane controls cleavage furrow formation during cytokinesis. AU - Su,Kuan-Chung, AU - Takaki,Tohru, AU - Petronczki,Mark, PY - 2011/10/10/received PY - 2011/11/09/revised PY - 2011/11/09/accepted PY - 2011/12/17/entrez PY - 2011/12/17/pubmed PY - 2012/2/14/medline SP - 1104 EP - 15 JF - Developmental cell JO - Dev. Cell VL - 21 IS - 6 N2 - In animal cells, formation of the cytokinetic furrow requires activation of the GTPase RhoA by the guanine nucleotide exchange factor Ect2. How Ect2, which is associated with the spindle midzone, controls RhoA activity at the equatorial cortex during anaphase is not understood. Here, we show that Ect2 concentrates at the equatorial membrane during cytokinesis in live cells. Ect2 membrane association requires a pleckstrin homology domain and a polybasic cluster that bind to phosphoinositide lipids. Both guanine nucleotide exchange function and membrane targeting of Ect2 are essential for RhoA activation and cleavage furrow formation in human cells. Membrane localization of Ect2 is spatially confined to the equator by centralspindlin, Ect2's spindle midzone anchor complex, and is temporally coordinated with chromosome segregation through the activation state of CDK1. We propose that targeting of Ect2 to the equatorial membrane represents a key step in the delivery of the cytokinetic signal to the cortex. SN - 1878-1551 UR - https://www.unboundmedicine.com/medline/citation/22172673/Targeting_of_the_RhoGEF_Ect2_to_the_equatorial_membrane_controls_cleavage_furrow_formation_during_cytokinesis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1534-5807(11)00513-2 DB - PRIME DP - Unbound Medicine ER -