Tags

Type your tag names separated by a space and hit enter

Relationship between ischemia/reperfusion injury and the stimulus of fibrogenesis in an experimental model: comparison among different preservation solutions.
Transplant Proc. 2011 Dec; 43(10):3634-7.TP

Abstract

BACKGROUND AND AIMS

Orthotopic liver transplantation (OLT) has been the standard treatment for end-stage acute and chronic liver disease. Ischemia-reperfusion (I/R) injury is one of the major causes of poor graft function early after OLT, and adversely influencing graft and patient survivals. It is unknown whether I/R injury influences liver fibrogenesis.

MATERIALS AND METHODS

Livers from 25 adult male Wistar rats were randomly assigned into 5 experimental groups according to the preservation solution: saline solution (SS); University of Wisconsin (UW) solution; Fructose 1, 6-biphosphate (FBP); S-Nitroso-N-Acetylcysteine (SNAC): or UW+SNAC (SNAC+UW). Aspartate aminotransferase (AST), alanine aminotransferase (ALT), and lactic dehydrogenase (LDH) were determined in preservation solution samples at 2, 4, and 6 hours. After 6 hours of cold ischemia, ex situ reperfusion was applied to the liver for 15 minutes. Serum AST, ALT, LDH, and renin levels were determined. Fresh liver slices were processed for histological studies, determination of thiobarbituric acid reactive substances, catalase, and glutathione, and expression of TGF-β1 and angiotensin II AT1 receptor.

RESULTS

AST was significantly lower during cold storage with UW than with the older media (P=.001); ALT was lower in the FBP group (P=.023) and LDH was lower in the FBP and SNAC groups (P=.007). After reperfusion, serum AST, ALT, LDH, and TBARS showed no significant differences among the groups. Catalase was significantly lower in the SS and FBP groups (P=.008 and P=.006, respectively). Compared with UW, glutathione concentrations were significantly higher in SS, FBP, and SNAC 200 (P=.004). Renin levels were significantly lower in the FBP group (P=.022). No histological signs of preservation injury were observed in the hepatic sample. No expressions were detected of TGF-β1 or AT1 receptor.

CONCLUSION

In this experimental model of early reperfusion injury, preservation changes related to higher levels of renin, which suggest its role in fibrogenesis. FBP was associated with lower renin levels than other solutions including UW.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Camacho VR
School of Medicine, Universidade Federal do Rio Grande do Sul/Hospital de Clínicas de Porto Alegre, Brazil. vrcamachoterra.com.br
de Fraga RS
No affiliation info available
Fraga RS
No affiliation info available
Cerski CT
No affiliation info available
de Oliveira JR
No affiliation info available
Oliveira JR
No affiliation info available
Álvares-da-Silva MR
No affiliation info available
Alvares-Dasilva MR
No affiliation info available

MeSH

AcetylcysteineAdenosineAlanine TransaminaseAllopurinolAnimalsAspartate AminotransferasesBiomarkersCatalaseDisease Models, AnimalFructosediphosphatesGlutathioneInsulinL-Lactate DehydrogenaseLiverLiver CirrhosisLiver TransplantationMaleOrgan Preservation SolutionsRaffinoseRatsRats, WistarReceptor, Angiotensin, Type 1ReninReperfusion InjuryThiobarbituric Acid Reactive SubstancesTime FactorsTransforming Growth Factor beta1

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22172818

Citation

Camacho, V R., et al. "Relationship Between Ischemia/reperfusion Injury and the Stimulus of Fibrogenesis in an Experimental Model: Comparison Among Different Preservation Solutions." Transplantation Proceedings, vol. 43, no. 10, 2011, pp. 3634-7.
Camacho VR, de Fraga RS, Fraga RS, et al. Relationship between ischemia/reperfusion injury and the stimulus of fibrogenesis in an experimental model: comparison among different preservation solutions. Transplant Proc. 2011;43(10):3634-7.
Camacho, V. R., de Fraga, R. S., Fraga, R. S., Cerski, C. T., de Oliveira, J. R., Oliveira, J. R., Álvares-da-Silva, M. R., & Alvares-Dasilva, M. R. (2011). Relationship between ischemia/reperfusion injury and the stimulus of fibrogenesis in an experimental model: comparison among different preservation solutions. Transplantation Proceedings, 43(10), 3634-7. https://doi.org/10.1016/j.transproceed.2011.08.114
Camacho VR, et al. Relationship Between Ischemia/reperfusion Injury and the Stimulus of Fibrogenesis in an Experimental Model: Comparison Among Different Preservation Solutions. Transplant Proc. 2011;43(10):3634-7. PubMed PMID: 22172818.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Relationship between ischemia/reperfusion injury and the stimulus of fibrogenesis in an experimental model: comparison among different preservation solutions. AU - Camacho,V R, AU - de Fraga,R S, AU - Fraga,R S, AU - Cerski,C T, AU - de Oliveira,J R, AU - Oliveira,J R, AU - Álvares-da-Silva,M R, AU - Alvares-Dasilva,M R, PY - 2011/04/02/received PY - 2011/08/29/accepted PY - 2011/12/17/entrez PY - 2011/12/17/pubmed PY - 2012/3/30/medline SP - 3634 EP - 7 JF - Transplantation proceedings JO - Transplant Proc VL - 43 IS - 10 N2 - BACKGROUND AND AIMS: Orthotopic liver transplantation (OLT) has been the standard treatment for end-stage acute and chronic liver disease. Ischemia-reperfusion (I/R) injury is one of the major causes of poor graft function early after OLT, and adversely influencing graft and patient survivals. It is unknown whether I/R injury influences liver fibrogenesis. MATERIALS AND METHODS: Livers from 25 adult male Wistar rats were randomly assigned into 5 experimental groups according to the preservation solution: saline solution (SS); University of Wisconsin (UW) solution; Fructose 1, 6-biphosphate (FBP); S-Nitroso-N-Acetylcysteine (SNAC): or UW+SNAC (SNAC+UW). Aspartate aminotransferase (AST), alanine aminotransferase (ALT), and lactic dehydrogenase (LDH) were determined in preservation solution samples at 2, 4, and 6 hours. After 6 hours of cold ischemia, ex situ reperfusion was applied to the liver for 15 minutes. Serum AST, ALT, LDH, and renin levels were determined. Fresh liver slices were processed for histological studies, determination of thiobarbituric acid reactive substances, catalase, and glutathione, and expression of TGF-β1 and angiotensin II AT1 receptor. RESULTS: AST was significantly lower during cold storage with UW than with the older media (P=.001); ALT was lower in the FBP group (P=.023) and LDH was lower in the FBP and SNAC groups (P=.007). After reperfusion, serum AST, ALT, LDH, and TBARS showed no significant differences among the groups. Catalase was significantly lower in the SS and FBP groups (P=.008 and P=.006, respectively). Compared with UW, glutathione concentrations were significantly higher in SS, FBP, and SNAC 200 (P=.004). Renin levels were significantly lower in the FBP group (P=.022). No histological signs of preservation injury were observed in the hepatic sample. No expressions were detected of TGF-β1 or AT1 receptor. CONCLUSION: In this experimental model of early reperfusion injury, preservation changes related to higher levels of renin, which suggest its role in fibrogenesis. FBP was associated with lower renin levels than other solutions including UW. SN - 1873-2623 UR - https://www.unboundmedicine.com/medline/citation/22172818/Relationship_between_ischemia/reperfusion_injury_and_the_stimulus_of_fibrogenesis_in_an_experimental_model:_comparison_among_different_preservation_solutions_ DB - PRIME DP - Unbound Medicine ER -