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Effects of anandamide on potassium channels in rat ventricular myocytes: a suppression of I(to) and augmentation of K(ATP) channels.
Am J Physiol Cell Physiol. 2012 Mar 15; 302(6):C924-30.AJ

Abstract

Anandamide is an endocannabinoid that has antiarrhythmic effects through inhibition of L-type Ca(2+) channels in cardiomyocytes. In this study, we investigated the electrophysiological effects of anandamide on K(+) channels in rat ventricular myocytes. Whole cell patch-clamp technique was used to record K(+) currents, including transient outward potassium current (I(to)), steady-state outward potassium current (I(ss)), inward rectifier potassium current (I(K1)), and ATP-sensitive potassium current (I(KATP)) in isolated rat cardiac ventricular myocytes. Anandamide decreased I(to) while increasing I(KATP) in a concentration-dependent manner but had no effect on I(ss) and I(K1) in isolated ventricular myocytes. Furthermore, anandamide shifted steady-state inactivation curve of I(to) to the left and shifted the recovery curve of I(to) to the right. However, neither cannabinoid 1 (CB(1)) receptor antagonist AM251 nor CB(2) receptor antagonist AM630 eliminated the inhibitory effect of anandamide on I(to). In addition, blockade of CB(2) receptors, but not CB(1) receptors, eliminated the augmentation effect of anandamide on I(KATP). These data suggest that anandamide suppresses I(to) through a non-CB(1) and non-CB(2) receptor-mediated pathway while augmenting I(KATP) through CB(2) receptors in ventricular myocytes.

Authors+Show Affiliations

Department of Physiology, Hebei Medical University, Shijiazhuang, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22173869

Citation

Li, Qian, et al. "Effects of Anandamide On Potassium Channels in Rat Ventricular Myocytes: a Suppression of I(to) and Augmentation of K(ATP) Channels." American Journal of Physiology. Cell Physiology, vol. 302, no. 6, 2012, pp. C924-30.
Li Q, Ma HJ, Song SL, et al. Effects of anandamide on potassium channels in rat ventricular myocytes: a suppression of I(to) and augmentation of K(ATP) channels. Am J Physiol Cell Physiol. 2012;302(6):C924-30.
Li, Q., Ma, H. J., Song, S. L., Shi, M., Ma, H. J., Li, D. P., & Zhang, Y. (2012). Effects of anandamide on potassium channels in rat ventricular myocytes: a suppression of I(to) and augmentation of K(ATP) channels. American Journal of Physiology. Cell Physiology, 302(6), C924-30. https://doi.org/10.1152/ajpcell.00228.2011
Li Q, et al. Effects of Anandamide On Potassium Channels in Rat Ventricular Myocytes: a Suppression of I(to) and Augmentation of K(ATP) Channels. Am J Physiol Cell Physiol. 2012 Mar 15;302(6):C924-30. PubMed PMID: 22173869.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of anandamide on potassium channels in rat ventricular myocytes: a suppression of I(to) and augmentation of K(ATP) channels. AU - Li,Qian, AU - Ma,Hui-Jie, AU - Song,Sheng-Li, AU - Shi,Min, AU - Ma,Hui-Juan, AU - Li,De-Pei, AU - Zhang,Yi, Y1 - 2011/12/14/ PY - 2011/12/17/entrez PY - 2011/12/17/pubmed PY - 2012/5/15/medline SP - C924 EP - 30 JF - American journal of physiology. Cell physiology JO - Am J Physiol Cell Physiol VL - 302 IS - 6 N2 - Anandamide is an endocannabinoid that has antiarrhythmic effects through inhibition of L-type Ca(2+) channels in cardiomyocytes. In this study, we investigated the electrophysiological effects of anandamide on K(+) channels in rat ventricular myocytes. Whole cell patch-clamp technique was used to record K(+) currents, including transient outward potassium current (I(to)), steady-state outward potassium current (I(ss)), inward rectifier potassium current (I(K1)), and ATP-sensitive potassium current (I(KATP)) in isolated rat cardiac ventricular myocytes. Anandamide decreased I(to) while increasing I(KATP) in a concentration-dependent manner but had no effect on I(ss) and I(K1) in isolated ventricular myocytes. Furthermore, anandamide shifted steady-state inactivation curve of I(to) to the left and shifted the recovery curve of I(to) to the right. However, neither cannabinoid 1 (CB(1)) receptor antagonist AM251 nor CB(2) receptor antagonist AM630 eliminated the inhibitory effect of anandamide on I(to). In addition, blockade of CB(2) receptors, but not CB(1) receptors, eliminated the augmentation effect of anandamide on I(KATP). These data suggest that anandamide suppresses I(to) through a non-CB(1) and non-CB(2) receptor-mediated pathway while augmenting I(KATP) through CB(2) receptors in ventricular myocytes. SN - 1522-1563 UR - https://www.unboundmedicine.com/medline/citation/22173869/Effects_of_anandamide_on_potassium_channels_in_rat_ventricular_myocytes:_a_suppression_of_I_to__and_augmentation_of_K_ATP__channels_ L2 - https://journals.physiology.org/doi/10.1152/ajpcell.00228.2011?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -